Product Info Summary
SKU: | A01171-1 |
---|---|
Size: | 100ul |
Reactive Species: | Human |
Host: | Rabbit |
Application: | ICC, WB |
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Product info
Product Name
Anti-Transcriptional activator GLI3 Gli3 Antibody
SKU/Catalog Number
A01171-1
Size
100ul
Form
Liquid
Description
Boster Bio Anti-Transcriptional activator GLI3 Gli3 Antibody catalog # A01171-1. Tested in WB,ICC applications. This antibody reacts with Human.
Storage & Handling
Store at -20°C for one year. For short term storage and frequent use, store at 4°C for up to one month. Avoid repeated freeze-thaw cycles.
Cite This Product
Anti-Transcriptional activator GLI3 Gli3 Antibody (Boster Biological Technology, Pleasanton CA, USA, Catalog # A01171-1)
Host
Rabbit
Contents
Rabbit IgG, 1mg/ml in PBS with 0.02% sodium azide, 50% glycerol, pH7.2
Clonality
Polyclonal
Isotype
IgG
Immunogen
Synthesized peptide derived from human COL7A1
*Blocking peptide can be purchased. Costs vary based on immunogen length. Contact us for pricing.
Cross-reactivity
No cross reactivity with other proteins.
Reactive Species
A01171-1 is reactive to GLI3 in Human
Applications
A01171-1 is guaranteed for ICC, WB Boster Guarantee
Observed Molecular Weight
Calculated molecular weight
169863 MW
Background of GLI-3
Sequence-specific DNA-binding protein that interacts with inducible viral and cellular enhancer elements to regulate transcription of selected genes. AP-2 factors bind to the consensus sequence 5'-GCCNNNGGC-3' and activate genes involved in a large spectrum of important biological functions including proper eye, face, body wall, limb and neural tube development. They also suppress a number of genes including MCAM/MUC18, C/EBP alpha and MYC. AP-2-alpha is the only AP-2 protein required for early morphogenesis of the lens vesicle. Together with the CITED2 coactivator, stimulates the PITX2 P1 promoter transcription activation. Associates with chromatin to the PITX2 P1 promoter region.
ShunJiang Yu, J. Biol. Chem., May 2003; 278: 19597 - 19602.
TT Chen, Mol. Cell. Biol., Jun 1997; 17: 3056 - 3064.
Oscar B. Goodman, J. Biol. Chem., Oct 1995; 270: 23768.
Didier Jean, J. Biol. Chem., Jun 1998; 273: 16501
Antibody Validation
Boster validates all antibodies on WB, IHC, ICC, Immunofluorescence, and ELISA with known positive control and negative samples to ensure specificity and high affinity, including thorough antibody incubations.
Assay dilution & Images
Assay Dilutions Recommendation
The recommendations below provide a starting point for assay optimization. The actual working concentration varies and should be decided by the user.
WB: 1:500-1:2,000
ICC: 1:50-1:100
Validation Images & Assay Conditions
![a01171 1 anti transcriptional activator gli3 gli3 antibody wb testing 1 a01171 1 anti transcriptional activator gli3 gli3 antibody wb testing 1](https://www.bosterbio.com/media/catalog/product/a/0/a01171-1-anti-transcriptional-activator-gli3-gli3-antibody-wb-testing-1.jpg)
Click image to see more details
Figure 1. Western blotting validation for Anti-Transcriptional activator GLI3 Gli3 Antibody A01171-1
Western blot analysis of Gli3 on PC-3M cell lysate using anti-Gli3 antibody at 1/1Protein Target Info & Infographic
Gene/Protein Information For GLI3 (Source: Uniprot.org, NCBI)
Gene Name
GLI3
Full Name
Transcriptional activator GLI3
Weight
169863 MW
Superfamily
GLI C2H2-type zinc-finger protein family
Alternative Names
ACLS; ACLSPAPAPHS; ADD; Bph; GCPS; GLI family zinc finger 3; GLI3 form of 190 kDa; GLI3 full length protein; GLI3; GLI-3; GLI3-190; GLI3FL; GLI-Kruppel family member GLI3; glioma-associated oncogene family zinc finger 3; Greig cephalopolysyndactyly syndrome; oncogene GLI3; PAP-A; PAPA1; PAPB; PAPBDNA-binding protein; Pdn; PHS; PPDIV; Xt; zinc finger protein GLI3 GLI3 ACLS, GCPS-190, GLI3FL, PAP-A, PAPA, PAPA1, PAPB, PHS, PPDIV, GLI3 GLI family zinc finger 3 transcriptional activator GLI3|GLI-Kruppel family member GLI3|glioma-associated oncogene family zinc finger 3|oncogene GLI3|zinc finger protein GLI3
*If product is indicated to react with multiple species, protein info is based on the gene entry specified above in "Species".For more info on GLI3, check out the GLI3 Infographic
![GLI3 infographic](/media/images/gene-infographic-example.jpg)
We have 30,000+ of these available, one for each gene! Check them out.
In this infographic, you will see the following information for GLI3: database IDs, superfamily, protein function, synonyms, molecular weight, chromosomal locations, tissues of expression, subcellular locations, post-translational modifications, and related diseases, research areas & pathways. If you want to see more information included, or would like to contribute to it and be acknowledged, please contact [email protected].
Specific Publications For Anti-Transcriptional activator GLI3 Gli3 Antibody (A01171-1)
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