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Product Info Summary
| SKU: | A00152-1 |
|---|---|
| Size: | 100 µl |
| Reactive Species: | Human |
| Host: | Rabbit |
| Application: | IHC, WB |
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Product info
Product Name
Anti-c-Cbl (Ab-700) Antibody
SKU/Catalog Number
A00152-1
Size
100 µl
Form
Liquid
Description
Boster Bio Anti-c-Cbl (Ab-700) Antibody (Catalog # A00152-1). Tested in WB, IHC applications. This antibody reacts with Human.
Storage & Handling
Store at -20°C for one year. For short term storage and frequent use, store at 4°C for up to one month. Avoid repeated freeze-thaw cycles.
Cite This Product
Anti-c-Cbl (Ab-700) Antibody (Boster Biological Technology, Pleasanton CA, USA, Catalog # A00152-1)
Host
Rabbit
Contents
Supplied at 1.0mg/mL in phosphate buffered saline (without Mg2+ and Ca2+), pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol.
Clonality
Polyclonal
Isotype
IgG
Immunogen
Peptide sequence around aa. 698~702 (T-E-Y-M-T) derived from Human c-Cbl.
*Blocking peptide can be purchased. Costs vary based on immunogen length. Contact us for pricing.
Reactive Species
A00152-1 is reactive to CBL in Human
Reconstitution
Observed Molecular Weight
39 kDa
Calculated molecular weight
33429 MW
Background of CBL
Participates in signal transduction in hematopoietic cells. Adapter protein that functions as a negative regulator of many signaling pathways that start from receptors at the cell surface. Acts as an E3 ubiquitin-protein ligase, which accepts ubiquitin from specific E2 ubiquitin-conjugating enzymes, and then transfers it to substrates promoting their degradation by the proteasome. Recognizes activated receptor tyrosine kinases, including PDGFA, EGF and CSF1, and terminates signaling.
Blake, T.J. et al. (1991) Oncogene 6, 653-657.
Thien, C.B. and Langdon, W.Y. (1998) Immunol. Cell Biol. 76, 473-482.
Kamei, T. et al. (2000) Int. J. Oncol. 17, 335-339.
Hunter, C. et al. (1999) J. Biol. Chem. 274, 2097-2106.
Antibody Validation
Boster validates all antibodies on WB, IHC, ICC, Immunofluorescence, and ELISA with known positive control and negative samples to ensure specificity and high affinity, including thorough antibody incubations.
Application & Images
Applications
A00152-1 is guaranteed for IHC, WB Boster Guarantee
Assay Dilutions Recommendation
The recommendations below provide a starting point for assay optimization. The actual working concentration varies and should be decided by the user.
Predicted MW: 120kd
Western blotting: 1:500~1:1000
Immunohistochemistry: 1:50~1:100
Validation Images & Assay Conditions
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Figure 1. Western blot analysis of CBL using anti-CBL antibody (A00152-1).
Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel) / 90V (Resolving gel) for 2-3 hours. The sample well of each lane was loaded with 50ug of sample under reducing conditions.
After Electrophoresis, proteins were transferred to a Nitrocellulose membrane at 150mA for 50-90 minutes. Blocked the membrane with 5% Non-fat Milk/ TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-CBL antigen affinity purified polyclonal antibody (Catalog # A00152-1) at 0.5 ug/mL overnight at 4°C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-Rabbit IgG-HRP secondary antibody at a dilution of 1:10000 for 1.5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit (Catalog # SA1022) with Tanon 5200 system. A specific band was detected for CBL.
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Figure 2. IHC analysis of CBL using anti-CBL antibody (A00152-1).
CBL was detected in paraffin-embedded section. Heat mediated antigen retrieval was performed in citrate buffer (pH6, epitope retrieval solution) for 20 mins. The tissue section was blocked with 10% goat serum. The tissue section was then incubated with 1ug/ml rabbit anti-CBL Antibody (A00152-1) overnight at 4°C. Biotinylated goat anti Rabbit IgG antibody was used as secondary antibody and incubated for 30 minutes at 37°C. The tissue section was developed using Strepavidin-Biotin-Complex (SABC)(Catalog # SA1022) with DAB as the chromogen.
Protein Target Info & Infographic
Gene/Protein Information For CBL (Source: Uniprot.org, NCBI)
Gene Name
CBL
Full Name
E3 ubiquitin-protein ligase CBL
Weight
33429 MW
Alternative Names
G1/S-specific cyclin-D1;Ccnd1;Cyl-1; CBL C-CBL2, FRA11B, NSLL, RNF55, CBL Cbl proto-oncogene E3 ubiquitin-protein ligase CBL|Cas-Br-M (murine) ecotropic retroviral transforming sequence|Cbl proto-oncogene, E3 ubiquitin protein ligase|RING finger protein 55|RING-type E3 ubiquitin transferase CBL|casitas B-lineage lymphoma proto-oncogene|fragile site, folic acid type, rare, fra(11)(q23.3)|oncogene CBL2|proto-oncogene c-Cbl|signal transduction protein CBL
*If product is indicated to react with multiple species, protein info is based on the gene entry specified above in "Species".For more info on CBL, check out the CBL Infographic
We have 30,000+ of these available, one for each gene! Check them out.
In this infographic, you will see the following information for CBL: database IDs, superfamily, protein function, synonyms, molecular weight, chromosomal locations, tissues of expression, subcellular locations, post-translational modifications, and related diseases, research areas & pathways. If you want to see more information included, or would like to contribute to it and be acknowledged, please contact [email protected].
Specific Publications For Anti-c-Cbl (Ab-700) Antibody (A00152-1)
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No publications found for A00152-1
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Customer Q&As
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5 Customer Q&As for Anti-c-Cbl (Ab-700) Antibody
Question
Do you have a BSA free version of anti-c-Cbl (Ab-700) antibody A00152-1 available?
Verified Customer
Verified customer
Asked: 2020-02-24
Answer
Thanks for your recent telephone inquiry. I can confirm that some lots of this anti-c-Cbl (Ab-700) antibody A00152-1 are BSA free. For now, these lots are available and we can make a BSA free formula for you free of charge. It will take 3 extra days to prepare. If you require this antibody BSA free again in future, please do not hesitate to contact me and I will be pleased to check which lots we have in stock that are BSA free.
Boster Scientific Support
Answered: 2020-02-24
Question
We appreciate helping with my inquiry over the phone. Here are the WB image, lot number and protocol we used for erythroleukemia using anti-c-Cbl (Ab-700) antibody A00152-1. Let me know if you need anything else.
K. Jones
Verified customer
Asked: 2020-01-08
Answer
Thank you for the data. You have provided everything we needed. Our lab team are working to resolve your inquiry as quickly as possible, and we appreciate your patience and understanding! Please let me know if there is anything you need in the meantime.
Boster Scientific Support
Answered: 2020-01-08
Question
Here is the WB image, lot number and protocol we used for erythroleukemia using anti-c-Cbl (Ab-700) antibody A00152-1. Please let me know if you require anything else.
Verified Customer
Verified customer
Asked: 2019-06-28
Answer
Thank you very much for the data. Our lab team are working to resolve this as quickly as possible, and we appreciate your patience and understanding! You have provided everything we needed. Please let me know if there is anything you need in the meantime.
Boster Scientific Support
Answered: 2019-06-28
Question
Does A00152-1 anti-c-Cbl (Ab-700) antibody work on parafin embedded sections? If so, which fixation method do you recommend we use (PFA, paraformaldehyde, other)?
Verified Customer
Verified customer
Asked: 2017-06-02
Answer
As indicated on the product datasheet, A00152-1 anti-c-Cbl (Ab-700) antibody as been validated on WB. It is best to use PFA for fixation because it has better tissue penetration ability. PFA needs to be prepared fresh before use. Long term stored PFA turns into formalin, as the PFA molecules congregate and become formalin.
Boster Scientific Support
Answered: 2017-06-02
Question
Does anti-c-Cbl (Ab-700) antibody A00152-1 work on canine IHC with erythroleukemia?
K. Roberts
Verified customer
Asked: 2013-08-26
Answer
Our lab technicians have not validated anti-c-Cbl (Ab-700) antibody A00152-1 on canine. You can run a BLAST between canine and the immunogen sequence of anti-c-Cbl (Ab-700) antibody A00152-1 to see if they may cross-react. If the sequence homology is close, then you can perform a pilot test. Keep in mind that since we have not validated canine samples, this use of the antibody is not covered by our guarantee. However we have an innovator award program that if you test this antibody and show it works in canine erythroleukemia in IHC, you can get your next antibody for free.
Boster Scientific Support
Answered: 2013-08-26
