Anti-Transcription factor SOX-2 SOX2 Antibody

Product Name

Anti-Transcription factor SOX-2 SOX2 Antibody

View all SOX2 Antibodies

SKU/Catalog Number

A00105

Size

0.1 mg

Form

Liquid

Description

Boster Bio Anti-Transcription factor SOX-2 SOX2 Antibody (Catalog # A00105). Tested in ELISA, WB, ICC, IHC-P, IF applications. This antibody reacts with Human, Mouse, Rat.

Storage & Handling

SOX2 antibody can be stored at 4°C for three months and -20°C, stable for up to one year. Avoid repeated freeze-thaw cycles. Antibodies should not be exposed to prolonged high temperatures.

Cite This Product

Anti-Transcription factor SOX-2 SOX2 Antibody (Boster Biological Technology, Pleasanton CA, USA, Catalog # A00105)

Host

Rabbit

Contents

SOX2 Antibody is supplied in PBS containing 0.02% sodium azide.

Clonality

Polyclonal

Isotype

IgG

Immunogen

SOX2 antibody was raised against a 15 amino acid synthetic peptide near the amino terminus of human SOX. The immunogen is located within the first 50 amino acids of SOX2.

*Blocking peptide can be purchased. Costs vary based on immunogen length. Contact us for pricing.

Cross-reactivity

TAU antibody is human, mouse and rat reactive. Multiple isoforms of TAU are known to exist; this antibody will only detect the two longest isoforms.

Reactive Species

A00105 is reactive to SOX2 in Human, Mouse, Rat

Reconstitution

Observed Molecular Weight

68 kDa

Calculated molecular weight

34310 MW

Background of SOX2

SOX2 is a member of the SRY-related HMG-box (SOX) family of transcription factors involved in the regulation of embryonic development and in the determination of cell fate. SOX2 is required for stem-cell maintenance in the central nervous system, and also regulates gene expression in the stomach. Mutations in this gene have been associated with optic nerve hypoplasia and with syndromic microphthalmia, a severe form of structural eye malformation. The role of SOX2 in embryonic development suggested that it might be useful in the creation of stem cells that might be useful in cell replacement therapies in the treatment of degenerative diseases. Artificial stem cells, termed induced pluripotent stem (iPS) cells, can be created by expressing SOX2 and the transcription factors POU5F1, Klf4 and Lin28 along with c-Myc in mouse fibroblasts. Other experiments have shown that iPS cells could be generated using expression plasmids expressing POU5F1, SOX2, KlfF4 and c-Myc, eliminating the need for virus introduction.

Antibody Validation

Boster validates all antibodies on WB, IHC, ICC, Immunofluorescence, and ELISA with known positive control and negative samples to ensure specificity and high affinity, including thorough antibody incubations.

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Applications

A00105 is guaranteed for ELISA, IF, IHC-P, ICC, WB Boster Guarantee

Assay Dilutions Recommendation

The recommendations below provide a starting point for assay optimization. The actual working concentration varies and should be decided by the user.

SOX2 antibody can be used for detection of SOX2 by Western blot at 1 - 2 μg/mL. Antibody can also be used for immunohistochemistry starting at 5 μg/mL and immunocytochemistry starting at 5 μg/mL. For immunofluorescence start at 20 μg/mL.
Antibody validated: Western Blot in mouse samples; Immunohistochemistry in mouse samples; Immunocytochemistry in mouse samples and Immunofluorescence in mouse samples. All other applications and species not yet tested. Optimal dilutions for each application should be determined by the researcher.

Validation Images & Assay Conditions

Gene/Protein Information For SOX2 (Source: Uniprot.org, NCBI)

Alternative Names

ANOP3, MCOPS3, Transcription factor SOX-2 SOX2 ANOP3, MCOPS3 SRY-box transcription factor 2 transcription factor SOX-2|SRY (sex determining region Y)-box 2|SRY-box 2|SRY-related HMG-box gene 2|sex determining region Y-box 2|transcription factor SOX2

*If product is indicated to react with multiple species, protein info is based on the gene entry specified above in "Species".

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