Anti-SOX2 (Transcription Factor) Monoclonal Antibody

Product Name

Anti-SOX2 (Transcription Factor) Monoclonal Antibody

View all SOX2 Antibodies

SKU/Catalog Number

M00105

Size

100ug/vial

Form

Liquid

Description

Boster Bio Anti-SOX2 (Transcription Factor) Monoclonal Antibody (Catalog # M00105). Tested in ELISA, WB, IHC applications. This antibody reacts with Human, Mouse.

Storage & Handling

Antibody with azide - store at 2 to 8°C. Antibody without azide - store at -20 to -80°C. Antibody is stable for 24 months. Non-hazardous. No MSDS required.

Cite This Product

Anti-SOX2 (Transcription Factor) Monoclonal Antibody (Boster Biological Technology, Pleasanton CA, USA, Catalog # M00105)

Host

Mouse

Contents

Prepared in 10mM PBS with 0.05% BSA & 0.05% azide. Also available WITHOUT BSA & azide at 1.0mg/ml.

Clonality

Monoclonal

Clone Number

Clone: SOX2/1792

Isotype

IgG2b, kappa

Immunogen

Recombinant fragment (within aa176-305) of human SOX2 protein (exact sequence is proprietary)

*Blocking peptide can be purchased. Costs vary based on immunogen length. Contact us for pricing.

Reactive Species

M00105 is reactive to SOX2 in Human, Mouse

Reconstitution

Restore with deionized water (or equivalent) for reconstitution volume of 100 µL

Calculated molecular weight

34310 MW

Background of SOX2

SOX2 is required for stem cell maintenance in the central nervous system, and it also regulates gene expression in the stomach. SOX2 is necessary for regulating multiple transcription factors that affect Oct 3/4 expression. An essential function of SOX2 is to stabilize embryonic stem cells in a pluripotent state by maintaining the requisite level of Oct 3/4 expression. Reportedly, SOX2 is associated with aggressive phenotypes of breast, head and neck, gastric, colorectal, bladder, and small cell lung cancers. However, SOX2 is expressed in a high percentage of lung squamous cell carcinomas and has been shown to be an independent favorable prognostic marker.

Antibody Validation

Boster validates all antibodies on WB, IHC, ICC, Immunofluorescence, and ELISA with known positive control and negative samples to ensure specificity and high affinity, including thorough antibody incubations.

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Applications

M00105 is guaranteed for ELISA, IHC, WB Boster Guarantee

Assay Dilutions Recommendation

The recommendations below provide a starting point for assay optimization. The actual working concentration varies and should be decided by the user.

ELISA (Use Ab at 2-4ug/ml for coating) (Order Ab without BSA)
Western Blot (1-2ug/ml)
Immunohistochemistry (Formalin-fixed) (1-2ug/ml for 30 min at RT) (Staining of formalin-fixed tissues requires heating tissue sections in 10mM Tris with 1mM EDTA, pH 9.0, for 45 min at 95°C followed by cooling at RT for 20 minutes)
Optimal dilution for a specific application should be determined.

Validation Images & Assay Conditions

Gene/Protein Information For SOX2 (Source: Uniprot.org, NCBI)

Alternative Names

Transcription factor SOX-2;SOX2; SOX2 ANOP3, MCOPS3 SRY-box transcription factor 2 transcription factor SOX-2|SRY (sex determining region Y)-box 2|SRY-box 2|SRY-related HMG-box gene 2|sex determining region Y-box 2|transcription factor SOX2

*If product is indicated to react with multiple species, protein info is based on the gene entry specified above in "Species".

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