Anti-TRAF6BP Rabbit Monoclonal Antibody

Product Name

Anti-TRAF6BP Rabbit Monoclonal Antibody

View all TAX1BP1 Antibodies

SKU/Catalog Number

M04336

Size

100 μl/vial

Form

Liquid

Description

Boster Bio Anti-TRAF6BP Rabbit Monoclonal Antibody catalog # M04336. Tested in WB, ICC/IF applications. This antibody reacts with Human, Mouse, Rat.

Storage & Handling

Store at -20°C for one year. For short term storage and frequent use, store at 4°C for up to one month. Avoid repeated freeze-thaw cycles.

Cite This Product

Anti-TRAF6BP Rabbit Monoclonal Antibody (Boster Biological Technology, Pleasanton CA, USA, Catalog # M04336)

Host

Rabbit

Contents

Rabbit IgG in phosphate buffered saline, pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol, 0.4-0.5mg/ml BSA.

Clonality

Monoclonal

Clone Number

23T27

Isotype

IgG

Immunogen

A synthesized peptide derived from human TRAF6BP

*Blocking peptide can be purchased. Costs vary based on immunogen length. Contact us for pricing.

Reactive Species

M04336 is reactive to TAX1BP1 in Human, Mouse, Rat

Reconstitution

Restore with deionized water (or equivalent) for reconstitution volume of 1.0 mL

Observed Molecular Weight

91 kDa

Calculated molecular weight

48075 MW

Antibody Validation

Boster validates all antibodies on WB, IHC, ICC, Immunofluorescence, and ELISA with known positive control and negative samples to ensure specificity and high affinity, including thorough antibody incubations.

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Applications

M04336 is guaranteed for IF, ICC, WB Boster Guarantee

Assay Dilutions Recommendation

The recommendations below provide a starting point for assay optimization. The actual working concentration varies and should be decided by the user.

WB 1:500-1:2000
ICC/IF 1:50-1:200

Positive Control

Validation Images & Assay Conditions

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Figure 1. Western blot analysis of TRAF6BP using anti-TRAF6BP antibody (M04336).
Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel) / 90V (Resolving gel) for 2-3 hours. The sample well of each lane was loaded with 30 ug of sample under reducing conditions.
Lane 1: human HepG2 whole cell lysates,
Lane 2: human RT4 whole cell lysates,
Lane 3: human SIHA whole cell lysates,
Lane 4: rat testis tissue lysates,
Lane 5: mouse testis tissue lysates.
After electrophoresis, proteins were transferred to a nitrocellulose membrane at 150 mA for 50-90 minutes. Blocked the membrane with 5% non-fat milk/TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-TRAF6BP antigen affinity purified monoclonal antibody (Catalog # M04336) at 1:500 overnight at 4°C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:5000 for 1.5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit (Catalog # EK1002) with Tanon 5200 system. A specific band was detected for TRAF6BP at approximately 91 kDa. The expected band size for TRAF6BP is at 91 kDa.

Gene/Protein Information For TAX1BP1 (Source: Uniprot.org, NCBI)

Alternative Names

T6BP; Tax1 (human T-cell leukemia virus type I) binding protein 1; tax1-binding protein 1; TRAF6-binding protein; TXBP151CALCOCO3 TAX1BP1 CALCOCO3, T6BP, TXBP151 Tax1 binding protein 1 tax1-binding protein 1|TRAF6-binding protein|Tax1 (human T-cell leukemia virus type I) binding protein 1

For more info on TAX1BP1, check out the TAX1BP1 Infographic

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In this infographic, you will see the following information for TAX1BP1: database IDs, superfamily, protein function, synonyms, molecular weight, chromosomal locations, tissues of expression, subcellular locations, post-translational modifications, and related diseases, research areas & pathways. If you want to see more information included, or would like to contribute to it and be acknowledged, please contact [email protected].

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