Click image to see more details
Product Info Summary
| SKU: | PA1514 |
|---|---|
| Size: | 100 μg/vial |
| Reactive Species: | Human, Mouse, Rat |
| Host: | Rabbit |
| Application: | Flow Cytometry, IF, IHC, WB |
Customers Who Bought This Also Bought
Product info
Product Name
Anti-Caveolin-1/CAV1 Antibody Picoband®
View all Caveolin-1 Antibodies
SKU/Catalog Number
PA1514
Size
100 μg/vial
Form
Lyophilized
Description
Boster Bio Anti-Caveolin-1/CAV1 Antibody catalog # PA1514. Tested in Flow Cytometry, IF, IHC, WB applications. This antibody reacts with Human, Mouse, Rat. The brand Picoband indicates this is a premium antibody that guarantees superior quality, high affinity, and strong signals with minimal background in Western blot applications. Only our best-performing antibodies are designated as Picoband, ensuring unmatched performance.
Storage & Handling
Store at -20˚C for one year from date of receipt. After reconstitution, at 4˚C for one month. It can also be aliquotted and stored frozen at -20˚C for six months. Avoid repeated freeze-thaw cycles.
Cite This Product
Anti-Caveolin-1/CAV1 Antibody Picoband® (Boster Biological Technology, Pleasanton CA, USA, Catalog # PA1514)
Host
Rabbit
Contents
Each vial contains 4 mg Trehalose, 0.9 mg NaCl and 0.2 mg Na2HPO4.
Clonality
Polyclonal
Isotype
Rabbit IgG
Immunogen
A synthetic peptide corresponding to a sequence at the C-terminus of human Caveolin-1, different from the related rat and mouse sequences by three amino acids.
*Blocking peptide can be purchased. Costs vary based on immunogen length. Contact us for pricing.
Cross-reactivity
No cross-reactivity with other proteins
Reactive Species
PA1514 is reactive to CAV1 in Human, Mouse, Rat
Reconstitution
Add 0.2ml of distilled water will yield a concentration of 500ug/ml.
Observed Molecular Weight
22 kDa
Calculated molecular weight
20472 MW
Background of Caveolin-1
CAV1, Caveolin-1, is a protein that in humans is encoded by the CAV1 gene. The CAV1 gene is mapped to 7q31.2. The scaffolding protein encoded by this gene is the main component of the caveolae plasma membranes found in most cell types. The protein links integrin subunits to the tyrosine kinase FYN, an initiating step in coupling integrins to the Ras-ERK pathway and promoting cell cycle progression. The gene is a tumor suppressor gene candidate and a negative regulator of the Ras-p42/44 MAP kinase cascade. CAV1 and CAV2 are located next to each other on chromosome 7 and express colocalizing proteins that form a stable hetero-oligomeric complex. By using alternative initiation codons in the same reading frame, two isoforms (alpha and beta) are encoded by a single transcript from this gene.
Antibody Validation
Boster validates all antibodies on WB, IHC, ICC, Immunofluorescence, and ELISA with known positive control and negative samples to ensure specificity and high affinity, including thorough antibody incubations.
Application & Images
Applications
PA1514 is guaranteed for Flow Cytometry, IF, IHC, WB Boster Guarantee
Assay Dilutions Recommendation
The recommendations below provide a starting point for assay optimization. The actual working concentration varies and should be decided by the user.
Western blot, 0.1-0.5μg/ml, Human, Mouse, Rat
Immunohistochemistry (Paraffin-embedded Section), 2-5μg/ml, Human, By Heat
Immunofluorescence, 5 μg/ml, Human
Flow Cytometry (Fixed), 1-3 μg/1x106 cells, Human
Positive Control
WB: human A549 whole cell, human Hela whole cell, human Hacat whole cell, human U87 whole cell, rat lung tissue, mouse lung tissue
IHC: human melanoma tissue
IF: human glioma tissue
FCM: A549 cell
Validation Images & Assay Conditions
Click image to see more details
Figure 1. Western blot analysis of Caveolin-1/CAV1 using anti-Caveolin-1/CAV1 antibody (PA1514).
Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel) / 90V (Resolving gel) for 2-3 hours. The sample well of each lane was loaded with 30 ug of sample under reducing conditions.
Lane 1: human A549 whole cell lysates,
Lane 2: human Hela whole cell lysates,
Lane 3: human Hacat whole cell lysates,
Lane 4: human U87 whole cell lysates,
Lane 5: rat lung tissue lysates,
Lane 6: mouse lung tissue lysates.
After electrophoresis, proteins were transferred to a nitrocellulose membrane at 150 mA for 50-90 minutes. Blocked the membrane with 5% non-fat milk/TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-Caveolin-1/CAV1 antigen affinity purified polyclonal antibody (Catalog # PA1514) at 0.5 μg/mL overnight at 4°C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:5000 for 1.5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit (Catalog # EK1002) with Tanon 5200 system. A specific band was detected for Caveolin-1/CAV1 at approximately 22 kDa. The expected band size for Caveolin-1/CAV1 is at 20 kDa.
Click image to see more details
Figure 2. IHC analysis of Caveolin-1/CAV1 using anti-Caveolin-1/CAV1 antibody (PA1514).
Caveolin-1/CAV1 was detected in a paraffin-embedded section of human melanoma tissue. Heat mediated antigen retrieval was performed in EDTA buffer (pH 8.0, epitope retrieval solution). The tissue section was blocked with 10% goat serum. The tissue section was then incubated with 2 μg/ml rabbit anti-Caveolin-1/CAV1 Antibody (PA1514) overnight at 4°C. Peroxidase Conjugated Goat Anti-rabbit IgG was used as secondary antibody and incubated for 30 minutes at 37°C. The tissue section was developed using HRP Conjugated Rabbit IgG Super Vision Assay Kit (Catalog # SV0002) with DAB as the chromogen.
Click image to see more details
Figure 4. Flow Cytometry analysis of A549 cells using anti-Caveolin-1/CAV1 antibody (PA1514).
Overlay histogram showing A549 cells stained with PA1514 (Blue line). To facilitate intracellular staining, cells were fixed with 4% paraformaldehyde and permeabilized with permeabilization buffer. The cells were blocked with 10% normal goat serum. And then incubated with rabbit anti-Caveolin-1/CAV1 Antibody (PA1514, 1 μg/1x106 cells) for 30 min at 20°C. DyLight®488 conjugated goat anti-rabbit IgG (BA1127, 5-10 μg/1x106 cells) was used as secondary antibody for 30 minutes at 20°C. Isotype control antibody (Green line) was rabbit IgG (1 μg/1x106) used under the same conditions. Unlabelled sample without incubation with primary antibody and secondary antibody (Red line) was used as a blank control.
Click image to see more details
Figure 3. IF analysis of Caveolin-1/CAV1 using anti-Caveolin-1/CAV1 antibody (PA1514).
Caveolin-1/CAV1 was detected in a paraffin-embedded section of human glioma tissue. Heat mediated antigen retrieval was performed in EDTA buffer (pH 8.0, epitope retrieval solution). The tissue section was blocked with 10% goat serum. The tissue section was then incubated with 5 μg/mL rabbit anti-Caveolin-1/CAV1 Antibody (PA1514) overnight at 4°C. DyLight®550 Conjugated Goat Anti-Rabbit IgG (BA1135) was used as secondary antibody at 1:500 dilution and incubated for 30 minutes at 37°C. The section was counterstained with DAPI. Visualize using a fluorescence microscope and filter sets appropriate for the label used.
Protein Target Info & Infographic
Gene/Protein Information For CAV1 (Source: Uniprot.org, NCBI)
Gene Name
CAV1
Full Name
Caveolin-1
Weight
20472 MW
Superfamily
caveolin family
Alternative Names
Caveolin-1;CAV1;CAV; CAV1 BSCL3, CGL3, LCCNS, MSTP085, PPH3, VIP21 caveolin 1 caveolin-1|caveolin 1, caveolae protein, 22kDa|cell growth-inhibiting protein 32
*If product is indicated to react with multiple species, protein info is based on the gene entry specified above in "Species".For more info on CAV1, check out the CAV1 Infographic
We have 30,000+ of these available, one for each gene! Check them out.
In this infographic, you will see the following information for CAV1: database IDs, superfamily, protein function, synonyms, molecular weight, chromosomal locations, tissues of expression, subcellular locations, post-translational modifications, and related diseases, research areas & pathways. If you want to see more information included, or would like to contribute to it and be acknowledged, please contact [email protected].
Specific Publications For Anti-Caveolin-1/CAV1 Antibody Picoband® (PA1514)
Hello CJ!
PA1514 has been cited in 3 publications:
*The publications in this section are manually curated by our staff scientists. They may differ from Bioz's machine gathered results. Both are accurate. If you find a publication citing this product but is missing from this list, please let us know we will issue you a thank-you coupon.
MicroRNA-199a-5p Affects Porcine Preadipocyte Proliferation and Differentiation
MicroRNA-199a-5p Affects Porcine Preadipocyte Proliferation and Differentiation
Wang Nn, Zhao Lj, Wu Ln, He Mf, Qu Jw, Zhao Yb, Zhao Wz, Li Js, Wang Jh. Asian Pac J Cancer Prev. 2013;14(9):4983-8. Mechanistic Analysis Of Taxol-Induced Multidrug Resistance In An Ovarian Cancer Cell Line.
Recommended Resources
Here are featured tools and databases that you might find useful.
- Boster's Pathways Library
- Protein Databases
- Bioscience Research Protocol Resources
- Data Processing & Analysis Software
- Photo Editing Software
- Scientific Literature Resources
- Research Paper Management Tools
- Molecular Biology Software
- Primer Design Tools
- Bioinformatics Tools
- Phylogenetic Tree Analysis
Customer Reviews
Have you used Anti-Caveolin-1/CAV1 Antibody Picoband®?
Submit a review and receive an Amazon gift card.
- $30 for a review with an image
0 Reviews For Anti-Caveolin-1/CAV1 Antibody Picoband®
Customer Q&As
Have a question?
Find answers in Q&As, reviews.
Can't find your answer?
Submit your question
5 Customer Q&As for Anti-Caveolin-1/CAV1 Antibody Picoband®
Question
Our lab used your anti-Caveolin-1/CAV1 antibody for IHC on lung in a previous experiment. I am using rat, and I plan to use the antibody for WB next. I am interested in examining lung as well as cervix carcinoma in our next experiment. Could you please give me some suggestion on which antibody would work the best for WB?
Verified Customer
Verified customer
Asked: 2019-11-21
Answer
I have checked the website and datasheets of our anti-Caveolin-1/CAV1 antibody and I see that PA1514 has been validated on rat in both IHC and WB. Thus PA1514 should work for your application. Our Boster satisfaction guarantee will cover this product for WB in rat even if the specific tissue type has not been validated. We do have a comprehensive range of products for WB detection and you can check out our website bosterbio.com to find out more information about them.
Boster Scientific Support
Answered: 2019-11-21
Question
you antibody using your anti-Caveolin-1/CAV1 antibody for response to calcium ion studies. Has this antibody been tested with western blotting on hela cell lysate? We would like to see some validation images before ordering.
Verified Customer
Verified customer
Asked: 2019-08-05
Answer
We appreciate your inquiry. This PA1514 anti-Caveolin-1/CAV1 antibody is validated on u87 cell lysate, hela cell lysate, a549 cell lysate, ht1080 cell lysate, mammary cancer tissue. It is guaranteed to work for IHC, ICC, WB in human, mouse, rat. Our Boster guarantee will cover your intended experiment even if the sample type has not been be directly tested.
Boster Scientific Support
Answered: 2019-08-05
Question
We are currently using anti-Caveolin-1/CAV1 antibody PA1514 for human tissue, and we are happy with the ICC results. The species of reactivity given in the datasheet says human, mouse, rat. Is it possible that the antibody can work on bovine tissues as well?
Verified Customer
Verified customer
Asked: 2019-07-16
Answer
The anti-Caveolin-1/CAV1 antibody (PA1514) has not been validated for cross reactivity specifically with bovine tissues, though there is a good chance of cross reactivity. We have an innovator award program that if you test this antibody and show it works in bovine you can get your next antibody for free. Please contact me if I can help you with anything.
Boster Scientific Support
Answered: 2019-07-16
Question
We have seen staining in mouse adipocyte. Are there any suggestions? Is anti-Caveolin-1/CAV1 antibody supposed to stain adipocyte positively?
R. Walker
Verified customer
Asked: 2019-05-31
Answer
Based on literature adipocyte does express CAV1. Based on Uniprot.org, CAV1 is expressed in visceral pleura, lung, brain lung, adipose tissue, adipocyte, brain, cervix carcinoma, liver, among other tissues. Regarding which tissues have CAV1 expression, here are a few articles citing expression in various tissues:
Adipocyte, Pubmed ID: 15242332
Adipose tissue, Pubmed ID: 15219854
Brain, Pubmed ID: 11865038, 12531427
Brain, and Lung, Pubmed ID: 15489334
Cervix carcinoma, Pubmed ID: 18669648, 20068231, 23186163
Liver, Pubmed ID: 24275569
Lung, Pubmed ID: 1360410
Boster Scientific Support
Answered: 2019-05-31
Question
My team were well pleased with the WB result of your anti-Caveolin-1/CAV1 antibody. However we have seen positive staining in visceral pleura golgi apparatus membrane using this antibody. Is that expected? Could you tell me where is CAV1 supposed to be expressed?
R. Bhatt
Verified customer
Asked: 2016-07-25
Answer
Based on literature, visceral pleura does express CAV1. Generally CAV1 expresses in golgi apparatus membrane. Regarding which tissues have CAV1 expression, here are a few articles citing expression in various tissues:
Adipocyte, Pubmed ID: 15242332
Adipose tissue, Pubmed ID: 15219854
Brain, Pubmed ID: 11865038, 12531427
Brain, and Lung, Pubmed ID: 15489334
Cervix carcinoma, Pubmed ID: 18669648, 20068231, 23186163
Liver, Pubmed ID: 24275569
Lung, Pubmed ID: 1360410
Boster Scientific Support
Answered: 2016-07-25
