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Product Info Summary
| SKU: | A01114-3 |
|---|---|
| Size: | 100 μg/vial |
| Reactive Species: | Human, Mouse, Rat |
| Host: | Rabbit |
| Application: | WB |
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Product info
Product Name
Anti-CD40L/CD40LG Antibody Picoband®
View all CD40 Ligand/TNFSF5 Antibodies
SKU/Catalog Number
A01114-3
Size
100 μg/vial
Form
Lyophilized
Description
Boster Bio Anti-CD40L/CD40LG Antibody Picoband® catalog # A01114-3. Tested in WB applications. This antibody reacts with Human, Mouse, Rat. The brand Picoband indicates this is a premium antibody that guarantees superior quality, high affinity, and strong signals with minimal background in Western blot applications. Only our best-performing antibodies are designated as Picoband, ensuring unmatched performance.
Storage & Handling
Store at -20˚C for one year from date of receipt. After reconstitution, at 4˚C for one month. It can also be aliquotted and stored frozen at -20˚C for six months. Avoid repeated freeze-thaw cycles.
Cite This Product
Anti-CD40L/CD40LG Antibody Picoband® (Boster Biological Technology, Pleasanton CA, USA, Catalog # A01114-3)
Host
Rabbit
Contents
Each vial contains 4mg Trehalose, 0.9mg NaCl, 0.2mg Na2HPO4, 0.05mg NaN3.
Clonality
Polyclonal
Isotype
Rabbit IgG
Immunogen
A synthetic peptide corresponding to a sequence in the middle region of human CD40L/CD40LG, which shares 82.8% and 79.3% amino acid (aa) sequence identity with mouse and rat CD40L/CD40LG, respectively.
*Blocking peptide can be purchased. Costs vary based on immunogen length. Contact us for pricing.
Cross-reactivity
No cross-reactivity with other proteins.
Reactive Species
A01114-3 is reactive to CD40LG in Human, Mouse, Rat
Reconstitution
Add 0.2ml of distilled water will yield a concentration of 500ug/ml.
Observed Molecular Weight
36 kDa
Calculated molecular weight
29.274kDa
Background of CD40 Ligand/TNFSF5
CD40 ligand (CD40L) is a type II membrane protein of 261 amino acids on activated T cells that induces B cell proliferation and immunoglobulin secretion. It has homology with tumour necrosis factor-alpha and -beta, and has important functions in B-cell activation and differentiation. Human CD40L with 5 exons, is mapped to the proximal region of the mouse X chromosome on Xq26.3-27.1, and can be detected on T cells but is absent from B cells and monocytes. Since CD40L is expressed on platelets and released from them on activation, its predictive value as a marker for clinical outcome and the therapeutic effect of inhibition of glycoprotein IIb /IIIa receptor in patients with acute coronary syndromes was investigated. The soluble CD40L may be involved in the process of restenosis and that it exerts its effect by triggering a complex group of inflammatory reactions on endothelial and mononuclear cells.CD40L plays a central role in the pathophysiology of acute coronary syndromes, and has a role in the pathogenesis of coronary artery lesions.
Antibody Validation
Boster validates all antibodies on WB, IHC, ICC, Immunofluorescence, and ELISA with known positive control and negative samples to ensure specificity and high affinity, including thorough antibody incubations.
Application & Images
Applications
A01114-3 is guaranteed for WB Boster Guarantee
Assay Dilutions Recommendation
The recommendations below provide a starting point for assay optimization. The actual working concentration varies and should be decided by the user.
Western blot, 0.1-0.5μg/ml
Positive Control
WB: human HL-60 whole cell, human U-87MG whole cell, rat liver tissue, rat stomach tissue, mouse spleen tissue, mouse thymus tissue
Validation Images & Assay Conditions
Click image to see more details
Figure 1. Western blot analysis of CD40LG using anti-CD40LG antibody (A01114-3).
Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel) / 90V (Resolving gel) for 2-3 hours. The sample well of each lane was loaded with 50ug of sample under reducing conditions.
Lane 1: human HL-60 whole cell lysates, Lane 2: human U-87MG whole cell lysates, Lane 3: rat liver tissue lysates, Lane 4: rat stomach tissue lysates, Lane 5: mouse spleen tissue lysates, Lane 6: mouse thymus tissue lysates.
After Electrophoresis, proteins were transferred to a Nitrocellulose membrane at 150mA for 50-90 minutes. Blocked the membrane with 5% Non-fat Milk/ TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-CD40LG antigen affinity purified polyclonal antibody (Catalog # A01114-3) at 0.5 μg/mL overnight at 4°C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:10000 for 1.5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit (Catalog # EK1002) with Tanon 5200 system. A specific band was detected for CD40LG at approximately 36KD. The expected band size for CD40LG is at 29KD.
Protein Target Info & Infographic
Gene/Protein Information For CD40LG (Source: Uniprot.org, NCBI)
Gene Name
CD40LG
Full Name
CD40 ligand
Weight
29.274kDa
Superfamily
tumor necrosis factor family
Alternative Names
CD40 ligand; CD40-L; T-cell antigen Gp39; TNF-related activation protein; TRAP; Tumor necrosis factor ligand superfamily member 5; CD154; CD40LG; CD40L; TNFSF5; TRAP CD40LG CD154, CD40L, HIGM1, IGM, IMD3, T-BAM, TNFSF5, TRAP, gp39, hCD40L CD40 ligand CD40 ligand|CD40 ligand|CD40-L|T-B cell-activating molecule|T-cell Gp39|TNF-related activation protein|tumor necrosis factor (ligand) superfamily member 5
*If product is indicated to react with multiple species, protein info is based on the gene entry specified above in "Species".For more info on CD40LG, check out the CD40LG Infographic
We have 30,000+ of these available, one for each gene! Check them out.
In this infographic, you will see the following information for CD40LG: database IDs, superfamily, protein function, synonyms, molecular weight, chromosomal locations, tissues of expression, subcellular locations, post-translational modifications, and related diseases, research areas & pathways. If you want to see more information included, or would like to contribute to it and be acknowledged, please contact [email protected].
Specific Publications For Anti-CD40L/CD40LG Antibody Picoband® (A01114-3)
Hello CJ!
A01114-3 has been cited in 1 publications:
*The publications in this section are manually curated by our staff scientists. They may differ from Bioz's machine gathered results. Both are accurate. If you find a publication citing this product but is missing from this list, please let us know we will issue you a thank-you coupon.
Yang G, Fang Z, Liu Y, Zhang H, Shi X, Ji Q, Lin Q, Lin R. Evid Based Complement Alternat Med. 2011;2011:930324. Doi: 10.1093/Ecam/Nep013. Epub 2011 Jun 5. Protective Effects Of Chinese Traditional Medicine Buyang Huanwu Decoction On Myocardial In...
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Customer Reviews
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Customer Q&As
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15 Customer Q&As for Anti-CD40L/CD40LG Antibody Picoband®
Question
Here is the WB image, lot number and protocol we used for blood using anti-CD40L/CD40LG antibody A01114-3. Please let me know if you require anything else.
Verified Customer
Verified customer
Asked: 2020-04-09
Answer
Thank you very much for the data. Our lab team are working to resolve this as quickly as possible, and we appreciate your patience and understanding! You have provided everything we needed. Please let me know if there is anything you need in the meantime.
Boster Scientific Support
Answered: 2020-04-09
Question
I have a question about product A01114-3, anti-CD40L/CD40LG antibody. I was wondering if it would be possible to conjugate this antibody with biotin. I would need it to be without BSA or sodium azide. I am planning on using a buffer exchange of sodium azide with PBS only. Would there be problems for me to conjugate the antibody and store it in -20 degrees in small aliquots?
Verified Customer
Verified customer
Asked: 2020-02-28
Answer
We do not recommend storing this antibody with PBS buffer only in -20 degrees. If you want to store it in -20 degrees it is best to add some cryoprotectant like glycerol. If you want carrier free A01114-3 anti-CD40L/CD40LG antibody, we can provide it to you in a special formula with trehalose and/or glycerol. These molecules will not interfere with conjugation chemistry and provide a good level of protection for the antibody from degradation. Please be sure to specify this in your purchase order.
Boster Scientific Support
Answered: 2020-02-28
Question
I was wanting to use your anti-CD40L/CD40LG antibody for WB for mouse blood on frozen tissues, but I want to know if it has been validated for this particular application. Has this antibody been validated and is this antibody a good choice for mouse blood identification?
Verified Customer
Verified customer
Asked: 2020-01-17
Answer
It shows on the product datasheet, A01114-3 anti-CD40L/CD40LG antibody has been tested for WB on human, mouse, rat tissues. We have an innovator award program that if you test this antibody and show it works in mouse blood in IHC-frozen, you can get your next antibody for free.
Boster Scientific Support
Answered: 2020-01-17
Question
I see that the anti-CD40L/CD40LG antibody A01114-3 works with WB, what is the protocol used to produce the result images on the product page?
Verified Customer
Verified customer
Asked: 2019-09-30
Answer
You can find protocols for WB on the "support/technical resources" section of our navigation menu. If you have any further questions, please send an email to [email protected]
Boster Scientific Support
Answered: 2019-09-30
Question
Is there a BSA free version of anti-CD40L/CD40LG antibody A01114-3 available?
Verified Customer
Verified customer
Asked: 2019-08-07
Answer
I appreciate your recent telephone inquiry. I can confirm that some lots of this anti-CD40L/CD40LG antibody A01114-3 are BSA free. For now, these lots are available and we can make a BSA free formula for you free of charge. It will take 3 extra days to prepare. If you require this antibody BSA free again in future, please do not hesitate to contact me and I will be pleased to check which lots we have in stock that are BSA free.
Boster Scientific Support
Answered: 2019-08-07
Question
Is this A01114-3 anti-CD40L/CD40LG antibody reactive to the isotypes of CD40LG?
F. Johnson
Verified customer
Asked: 2019-06-26
Answer
The immunogen of A01114-3 anti-CD40L/CD40LG antibody is A synthetic peptide corresponding to a sequence of human CD40L/CD40LG(KEETKKENSFEMQKGDQNPQIAAHVISEA). Could you tell me which isotype you are interested in so I can help see if the immunogen is part of this isotype?
Boster Scientific Support
Answered: 2019-06-26
Question
I am looking for using your anti-CD40L/CD40LG antibody for inflammatory response studies. Has this antibody been tested with western blotting on mouse thymus tissue? We would like to see some validation images before ordering.
Verified Customer
Verified customer
Asked: 2019-05-30
Answer
Thanks for your inquiry. This A01114-3 anti-CD40L/CD40LG antibody is tested on rat stomach tissue, liver tissue, mouse thymus tissue, spleen tissue. It is guaranteed to work for WB in human, mouse, rat. Our Boster guarantee will cover your intended experiment even if the sample type has not been be directly tested.
Boster Scientific Support
Answered: 2019-05-30
Question
Would anti-CD40L/CD40LG antibody A01114-3 work for WB with blood?
Verified Customer
Verified customer
Asked: 2019-05-23
Answer
According to the expression profile of blood, CD40LG is highly expressed in blood. So, it is likely that anti-CD40L/CD40LG antibody A01114-3 will work for WB with blood.
Boster Scientific Support
Answered: 2019-05-23
Question
We have seen staining in rat blood. What should we do? Is anti-CD40L/CD40LG antibody supposed to stain blood positively?
Verified Customer
Verified customer
Asked: 2018-10-09
Answer
Based on literature blood does express CD40LG. Based on Uniprot.org, CD40LG is expressed in leukocyte, blood, among other tissues. Regarding which tissues have CD40LG expression, here are a few articles citing expression in various tissues:
Blood, Pubmed ID: 15489334
Boster Scientific Support
Answered: 2018-10-09
Question
We are currently using anti-CD40L/CD40LG antibody A01114-3 for human tissue, and we are happy with the WB results. The species of reactivity given in the datasheet says human, mouse, rat. Is it true that the antibody can work on bovine tissues as well?
Verified Customer
Verified customer
Asked: 2018-03-05
Answer
The anti-CD40L/CD40LG antibody (A01114-3) has not been validated for cross reactivity specifically with bovine tissues, though there is a good chance of cross reactivity. We have an innovator award program that if you test this antibody and show it works in bovine you can get your next antibody for free. Please contact me if I can help you with anything.
Boster Scientific Support
Answered: 2018-03-05
Question
My boss were well pleased with the WB result of your anti-CD40L/CD40LG antibody. However we have been able to see positive staining in leukocyte cell membrane using this antibody. Is that expected? Could you tell me where is CD40LG supposed to be expressed?
J. Brown
Verified customer
Asked: 2017-12-22
Answer
According to literature, leukocyte does express CD40LG. Generally CD40LG expresses in cell membrane. Regarding which tissues have CD40LG expression, here are a few articles citing expression in various tissues:
Blood, Pubmed ID: 15489334
Boster Scientific Support
Answered: 2017-12-22
Question
Would A01114-3 anti-CD40L/CD40LG antibody work on parafin embedded sections? If so, which fixation method do you recommend we use (PFA, paraformaldehyde, other)?
Verified Customer
Verified customer
Asked: 2017-10-19
Answer
You can see on the product datasheet, A01114-3 anti-CD40L/CD40LG antibody as been tested on WB. It is best to use PFA for fixation because it has better tissue penetration ability. PFA needs to be prepared fresh before use. Long term stored PFA turns into formalin, as the PFA molecules congregate and become formalin.
Boster Scientific Support
Answered: 2017-10-19
Question
My lab would like to test anti-CD40L/CD40LG antibody A01114-3 on mouse blood for research purposes, then I may be interested in using anti-CD40L/CD40LG antibody A01114-3 for diagnostic purposes as well. Is the antibody suitable for diagnostic purposes?
M. Thomas
Verified customer
Asked: 2016-07-18
Answer
The products we sell, including anti-CD40L/CD40LG antibody A01114-3, are only intended for research use. They would not be suitable for use in diagnostic work. If you have the means to develop a product into diagnostic use, and are interested in collaborating with us and develop our product into an IVD product, please contact us for more discussions.
Boster Scientific Support
Answered: 2016-07-18
Question
Is a blocking peptide available for product anti-CD40L/CD40LG antibody (A01114-3)?
C. Evans
Verified customer
Asked: 2015-07-22
Answer
We do provide the blocking peptide for product anti-CD40L/CD40LG antibody (A01114-3). If you would like to place an order for it please contact [email protected] and make a special request.
Boster Scientific Support
Answered: 2015-07-22
Question
I appreciate helping with my inquiry over the phone. Here are the WB image, lot number and protocol we used for blood using anti-CD40L/CD40LG antibody A01114-3. Let me know if you need anything else.
N. Brown
Verified customer
Asked: 2014-10-01
Answer
I appreciate the data. You have provided everything we needed. Our lab team are working to resolve your inquiry as quickly as possible, and we appreciate your patience and understanding! Please let me know if there is anything you need in the meantime.
Boster Scientific Support
Answered: 2014-10-01
