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Product Info Summary
| SKU: | M00320 |
|---|---|
| Size: | 100 μl |
| Reactive Species: | Human |
| Host: | Rabbit |
| Application: | IP, IHC, WB |
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Product info
Product Name
Anti-TPO Monoclonal Antibody
View all Thyroid Peroxidase Antibodies
SKU/Catalog Number
M00320
Size
100 μl
Form
Liquid
Description
Boster Bio Anti-TPO Monoclonal Antibody catalog # M00320. Tested in WB, IHC, IP applications. This antibody reacts with Human.
Storage & Handling
Store at -20°C for one year. For short term storage and frequent use, store at 4°C for up to one month. Avoid repeated freeze-thaw cycles.
Cite This Product
Anti-TPO Monoclonal Antibody (Boster Biological Technology, Pleasanton CA, USA, Catalog # M00320)
Host
Rabbit
Contents
Rabbit IgG in phosphate buffered saline, pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol, 0.4-0.5mg/ml BSA.
Clonality
Monoclonal
Clone Number
AFFI-20
Isotype
Rabbit IgG
Immunogen
A synthesized peptide derived from human TPO Iodination and coupling of the hormonogenic tyrosines in thyroglobulin to yield the thyroid hormones T (3) and T (4) .
*Blocking peptide can be purchased. Costs vary based on immunogen length. Contact us for pricing.
Reactive Species
M00320 is reactive to TPO in Human
Reconstitution
Restore with deionized water (or equivalent) for reconstitution volume of 1.0 mL
Observed Molecular Weight
84 kDa
Calculated molecular weight
95751 MW
Antibody Validation
Boster validates all antibodies on WB, IHC, ICC, Immunofluorescence, and ELISA with known positive control and negative samples to ensure specificity and high affinity, including thorough antibody incubations.
Application & Images
Applications
M00320 is guaranteed for IP, IHC, WB Boster Guarantee
Assay Dilutions Recommendation
The recommendations below provide a starting point for assay optimization. The actual working concentration varies and should be decided by the user.
WB 1:500-1:2000
IHC 1:50-1:200
IP 1:40
Positive Control
WB: Human thyroid
Validation Images & Assay Conditions
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Western blot analysis of TPO expression in Human thyroid lysate.
Protein Target Info & Infographic
Gene/Protein Information For TPO (Source: Uniprot.org, NCBI)
Gene Name
TPO
Full Name
Thyroid peroxidase
Weight
95751 MW
Superfamily
peroxidase family
Alternative Names
DNA (cytosine-5)-methyltransferase 3B;Dnmt3b;2.1.1.37;DNA methyltransferase HsaIIIB;DNA MTase HsaIIIB;M.HsaIIIB;DNMT3B; TPO MSA, TDH2A, TPX thyroid peroxidase thyroid peroxidase|thyroid microsomal |thyroperoxidase
*If product is indicated to react with multiple species, protein info is based on the gene entry specified above in "Species".For more info on TPO, check out the TPO Infographic
We have 30,000+ of these available, one for each gene! Check them out.
In this infographic, you will see the following information for TPO: database IDs, superfamily, protein function, synonyms, molecular weight, chromosomal locations, tissues of expression, subcellular locations, post-translational modifications, and related diseases, research areas & pathways. If you want to see more information included, or would like to contribute to it and be acknowledged, please contact [email protected].
Specific Publications For Anti-TPO Monoclonal Antibody (M00320)
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No publications found for M00320
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Customer Reviews
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Customer Q&As
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4 Customer Q&As for Anti-TPO Monoclonal Antibody
Question
I appreciate helping with my inquiry over the phone. Here are the WB image, lot number and protocol we used for right lobe of thyroid gland using anti-TPO Monoclonal antibody M00320. Let me know if you need anything else.
Verified Customer
Verified customer
Asked: 2019-10-25
Answer
I appreciate the data. You have provided everything we needed. Our lab team are working to resolve your inquiry as quickly as possible, and we appreciate your patience and understanding! Please let me know if there is anything you need in the meantime.
Boster Scientific Support
Answered: 2019-10-25
Question
Is this M00320 anti-TPO Monoclonal antibody reactive to the isotypes of TPO?
Verified Customer
Verified customer
Asked: 2019-09-18
Answer
The immunogen of M00320 anti-TPO Monoclonal antibody is A synthesized peptide derived from human TPO Iodination and coupling of the hormonogenic tyrosines in thyroglobulin to yield the thyroid hormones T (3) and T (4) . Could you tell me which isotype you are interested in so I can help see if the immunogen is part of this isotype?
Boster Scientific Support
Answered: 2019-09-18
Question
I have attached the WB image, lot number and protocol we used for right lobe of thyroid gland using anti-TPO Monoclonal antibody M00320. Please let me know if you require anything else.
Verified Customer
Verified customer
Asked: 2018-12-17
Answer
Thank you very much for the data. Our lab team are working to resolve this as quickly as possible, and we appreciate your patience and understanding! You have provided everything we needed. Please let me know if there is anything you need in the meantime.
Boster Scientific Support
Answered: 2018-12-17
Question
I see that the anti-TPO Monoclonal antibody M00320 works with WB, what is the protocol used to produce the result images on the product page?
P. Miller
Verified customer
Asked: 2017-03-09
Answer
You can find protocols for WB on the "support/technical resources" section of our navigation menu. If you have any further questions, please send an email to [email protected]
Boster Scientific Support
Answered: 2017-03-09
