Anti-TOM70 TOMM70 Antibody

Product Name

Anti-TOM70 TOMM70 Antibody

View all TOM70 Antibodies

SKU/Catalog Number

A09172

Size

0.1 mg

Form

Liquid

Description

Boster Bio Anti-TOM70 TOMM70 Antibody (Catalog # A09172). Tested in ELISA, WB, IHC-P, IF applications. This antibody reacts with Human, Mouse, Rat.

Storage & Handling

TOM70 antibody can be stored at 4°C for three months and -20°C, stable for up to one year. Avoid repeated freeze-thaw cycles. Antibodies should not be exposed to prolonged high temperatures.

Cite This Product

Anti-TOM70 TOMM70 Antibody (Boster Biological Technology, Pleasanton CA, USA, Catalog # A09172)

Host

Rabbit

Contents

TOM70 Antibody is supplied in PBS containing 0.02% sodium azide.

Clonality

Polyclonal

Isotype

IgG

Immunogen

Anti-TOM70 antibody was raised against a peptide corresponding to 17 amino acids near the amino terminus of human TOM70. The immunogen is located within amino acids 50-100 of TOM70.

*Blocking peptide can be purchased. Costs vary based on immunogen length. Contact us for pricing.

Cross-reactivity

At least two isoforms of TOM70 are known to exist; this antibody will only detect the larger isoform.

Reactive Species

A09172 is reactive to TOMM70A in Human, Mouse, Rat

Reconstitution

Observed Molecular Weight

68 kDa

Calculated molecular weight

67455 MW

Background of TOM70

The translocase of outer mitochondrial membrane (TOM) complex is a multisubunit complex involved in the recognition, unfolding, and translocation of preproteins into the mitochondria. TOM70, an important member of the TOM complex, contains a tetratricopeptide repeat domain similar to those found in cytosolic chaperones such as Hsp90 and Hsc70 and provides a docking site for these proteins. This interaction is thought to be a critical first step in the TOM70-dependent mitochondrial import, followed by contact between the preprotein and TOM70. After targeting to TOM70, preproteins are translocated through the outer membrane via the TOM40 import pore complex. The precise mechanism of how preproteins progress from TOM70 to TOM40 to full translocation is still unclear.

Antibody Validation

Boster validates all antibodies on WB, IHC, ICC, Immunofluorescence, and ELISA with known positive control and negative samples to ensure specificity and high affinity, including thorough antibody incubations.

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Applications

A09172 is guaranteed for ELISA, IF, IHC-P, WB Boster Guarantee

Assay Dilutions Recommendation

The recommendations below provide a starting point for assay optimization. The actual working concentration varies and should be decided by the user.

WB: 1-4 μg/mL; IHC: 2.5 μg/mL; IF: 20 μg/mL.
Antibody validated: Western Blot in human, mouse and rat samples; Immunohistochemistry and Immunofluorescence in mouse samples. All other applications and species not yet tested. Optimal dilutions for each application should be determined by the researcher.

Validation Images & Assay Conditions

Gene/Protein Information For TOMM70A (Source: Uniprot.org, NCBI)

Alternative Names

KIAA0719, TOM70, Mitochondrial import receptor subunit TOM70, Mitochondrial precursor proteins import receptor TOMM70 TOMM70A, Tom70 translocase of outer mitochondrial membrane 70 mitochondrial import receptor subunit TOM70|mitochondrial precursor proteins import receptor|translocase of outer membrane 70 kDa subunit|translocase of outer mitochondrial membrane 70 homolog A|translocase of outer mitochondrial membrane protein 70

*If product is indicated to react with multiple species, protein info is based on the gene entry specified above in "Species".

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