Anti-ALIX/PDCD6IP Antibody Picoband™(monoclonal, 14D10)

Alix antibody

Boster Bio Anti-ALIX/PDCD6IP Antibody Picoband™ (monoclonal, 14D10) catalog # M01751-1. Tested in Flow Cytometry, IF, IHC, ICC, WB applications. This antibody reacts with Human, Mouse, Rat. Cited in 1 publication(s).

Product Info Summary

SKU: M01751-1
Size: 100 μg/vial
Reactive Species: Human, Mouse, Rat
Host: Mouse
Application: Flow Cytometry, IF, IHC, ICC, WB

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Product Name

Anti-ALIX/PDCD6IP Antibody Picoband™(monoclonal, 14D10)

View all Alix Antibodies

SKU/Catalog Number

M01751-1

Size

100 μg/vial

Form

Lyophilized

Description

Boster Bio Anti-ALIX/PDCD6IP Antibody Picoband™ (monoclonal, 14D10) catalog # M01751-1. Tested in Flow Cytometry, IF, IHC, ICC, WB applications. This antibody reacts with Human, Mouse, Rat.

Storage & Handling

Store at -20˚C for one year from date of receipt. After reconstitution, at 4˚C for one month. It can also be aliquotted and stored frozen at -20˚C for six months. Avoid repeated freeze-thaw cycles.

Cite This Product

Anti-ALIX/PDCD6IP Antibody Picoband™(monoclonal, 14D10) (Boster Biological Technology, Pleasanton CA, USA, Catalog # M01751-1)

Host

Mouse

Contents

Each vial contains 4mg Trehalose, 0.9mg NaCl, 0.2mg Na2HPO4, 0.05mg NaN3.

Clonality

Monoclonal

Clone Number

14D10

Isotype

Mouse IgG2b

Immunogen

E.coli-derived human PDCD6IP recombinant protein (Position: A2-D330). Human PDCD6IP shares 96.7% and 95.2% amino acid  (aa) sequence identity with mouse and rat PDCD6IP, respectively.

*Blocking peptide can be purchased. Costs vary based on immunogen length. Contact us for pricing.

Cross-reactivity

No cross-reactivity with other proteins.

Reactive Species

M01751-1 is reactive to PDCD6IP in Human, Mouse, Rat

Applications

M01751-1 is guaranteed for Flow Cytometry, IF, IHC, ICC, WB Boster Guarantee

Observed Molecular Weight

96-100 kDa

Calculated molecular weight

96.023kDa

Background of Alix

Programmed cell death 6-interacting protein is a protein that in humans is encoded by the PDCD6IP gene. This gene encodes a protein that functions within the ESCRT pathway in the abscission stage of cytokinesis, in intralumenal endosomal vesicle formation, and in enveloped virus budding. Studies using mouse cells have shown that overexpression of this protein can block apoptosis. In addition, the product of this gene binds to the product of the PDCD6 gene, a protein required for apoptosis, in a calcium-dependent manner. This gene product also binds to endophilins, proteins that regulate membrane shape during endocytosis. Overexpression of this gene product and endophilins results in cytoplasmic vacuolization, which may be partly responsible for the protection against cell death. Several alternatively spliced transcript variants encoding different isoforms have been found for this gene.

Antibody Validation

Boster validates all antibodies on WB, IHC, ICC, Immunofluorescence, and ELISA with known positive control and negative samples to ensure specificity and high affinity, including thorough antibody incubations.

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Innovating Scientists Reward

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Reconsitution

Add 0.2ml of distilled water will yield a concentration of 500μg/ml.

Assay Dilutions Recommendation

The recommendations below provide a starting point for assay optimization. The actual working concentration varies and should be decided by the user.

"Western blot, 0.1-0.5μg/ml, Human, Mouse, Rat
Immunohistochemistry (Paraffin-embedded Section), 0.5-1μg/ml, Human, Mouse, Rat, By Heat
Immunocytochemistry/Immunofluorescence, 2μg/ml, Human
Flow Cytometry, 1-3μg/1x106, Human
"

Validation Images & Assay Conditions

Gene/Protein Information For PDCD6IP (Source: Uniprot.org, NCBI)

Gene Name

PDCD6IP

Full Name

Programmed cell death 6-interacting protein

Weight

96.023kDa

Alternative Names

AIP1DRIP4; ALG-2 interacting protein 1; ALG-2 interacting protein X; ALG-2-interacting protein 1; alinx; Alix; apoptosis-linked gene 2-interacting protein X; dopamine receptor interacting protein 4; HP95; KIAA1375; MGC17003; PDCD6-interacting protein; programmed cell death 6 interacting protein; programmed cell death 6-interacting protein PDCD6IP AIP1, ALIX, DRIP4, HP95 programmed cell death 6 interacting protein programmed cell death 6-interacting protein|ALG-2 interacting protein 1|ALG-2-interacting protein X|PDCD6-interacting protein|apoptosis-linked gene 2-interacting protein X|dopamine receptor interacting protein 4

*If product is indicated to react with multiple species, protein info is based on the gene entry specified above in "Species".

For more info on PDCD6IP, check out the PDCD6IP Infographic

PDCD6IP infographic

We have 30,000+ of these available, one for each gene! Check them out.

In this infographic, you will see the following information for PDCD6IP: database IDs, superfamily, protein function, synonyms, molecular weight, chromosomal locations, tissues of expression, subcellular locations, post-translational modifications, and related diseases, research areas & pathways. If you want to see more information included, or would like to contribute to it and be acknowledged, please contact [email protected].

Hello CJ!

M01751-1 has been cited in 1 publications:

*The publications in this section are manually curated by our staff scientists. They may differ from Bioz's machine gathered results. Both are accurate. If you find a publication citing this product but is missing from this list, please let us know we will issue you a thank-you coupon.

Cao G,Meng X, Han X,Li J.Exosomes derived from circRNA Rtn4-modified BMSCs attenuate TNF-α-induced cytotoxicity and apoptosis in murine MC3T3-E1 cells by sponging miR-146a.Biosci Rep.2020 May 29;40(5): BSR20193436.doi:10.1042/BSR20193436.PMID:32400849;PMC
Species: Mouse
M01751-1 usage in article: APP:WB, SAMPLE:MC3T3-E1 CELLS, DILUTION:NA

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5 Customer Q&As for Anti-ALIX/PDCD6IP Antibody Picoband™(monoclonal, 14D10)

Question

My team were well pleased with the WB result of your anti-ALIX/PDCD6IP antibody(monoclonal, 14D10). However we have been able to see positive staining in melanoma cytoplasm using this antibody. Is that expected? Could you tell me where is PDCD6IP supposed to be expressed?

Verified Customer

Verified customer

Asked: 2019-11-04

Answer

From literature, melanoma does express PDCD6IP. Generally PDCD6IP expresses in cytoplasm, cytosol. Regarding which tissues have PDCD6IP expression, here are a few articles citing expression in various tissues:
Brain, Pubmed ID: 10718198
Cervix carcinoma, Pubmed ID: 18669648, 20068231
Cervix carcinoma, and Erythroleukemia, Pubmed ID: 23186163
Colon carcinoma, Pubmed ID: 24129315
Liver, Pubmed ID: 24275569
Lymph, and Testis, Pubmed ID: 15489334
Melanoma, Pubmed ID: 17081065
Placenta, Pubmed ID: 14702039

Boster Scientific Support

Answered: 2019-11-04

Question

We ordered your anti-ALIX/PDCD6IP antibody(monoclonal, 14D10) for IHC-P on cervix carcinoma erythroleukemia a few months ago. I am using rat, and We want to use the antibody for IF next. My question regards examining cervix carcinoma erythroleukemia as well as placenta in our next experiment. Could give a recommendation on which antibody would work the best for IF?

Verified Customer

Verified customer

Asked: 2018-12-31

Answer

I took a look at the website and datasheets of our anti-ALIX/PDCD6IP antibody(monoclonal, 14D10) and it appears that M01751-1 has been validated on rat in both IHC-P and IF. Thus M01751-1 should work for your application. Our Boster satisfaction guarantee will cover this product for IF in rat even if the specific tissue type has not been validated. We do have a comprehensive range of products for IF detection and you can check out our website bosterbio.com to find out more information about them.

Boster Scientific Support

Answered: 2018-12-31

Question

We are currently using anti-ALIX/PDCD6IP antibody(monoclonal, 14D10) M01751-1 for human tissue, and we are well pleased with the ICC results. The species of reactivity given in the datasheet says human, mouse, rat. Is it likely that the antibody can work on monkey tissues as well?

Verified Customer

Verified customer

Asked: 2018-03-07

Answer

The anti-ALIX/PDCD6IP antibody(monoclonal, 14D10) (M01751-1) has not been validated for cross reactivity specifically with monkey tissues, but there is a good chance of cross reactivity. We have an innovator award program that if you test this antibody and show it works in monkey you can get your next antibody for free. Please contact me if I can help you with anything.

Boster Scientific Support

Answered: 2018-03-07

Question

I was wanting to use using your anti-ALIX/PDCD6IP antibody(monoclonal, 14D10) for viral life cycle studies. Has this antibody been tested with western blotting on intestinal cancer tissue? We would like to see some validation images before ordering.

N. Huang

Verified customer

Asked: 2016-11-30

Answer

I appreciate your inquiry. This M01751-1 anti-ALIX/PDCD6IP antibody(monoclonal, 14D10) is validated on human hela, hela whole cell lysates, hepg2 whole cell lysates, jurkat whole cell lysates, k562 whole cell lysates, rat testis tissue, mouse testis tissue, nih3t3 whole cell lysates, mammary cancer tissue, intestinal cancer tissue, u20s cells, a431 cells. It is guaranteed to work for Flow Cytometry, IF, IHC-P, ICC, WB in human, mouse, rat. Our Boster guarantee will cover your intended experiment even if the sample type has not been be directly tested.

Boster Scientific Support

Answered: 2016-11-30

Question

We have observed staining in human cervix carcinoma. What should we do? Is anti-ALIX/PDCD6IP antibody(monoclonal, 14D10) supposed to stain cervix carcinoma positively?

M. Banerjee

Verified customer

Asked: 2015-11-04

Answer

According to literature cervix carcinoma does express PDCD6IP. According to Uniprot.org, PDCD6IP is expressed in corpus callosum, lymph testis, osteosarcoma, placenta, brain, melanoma, cervix carcinoma, cervix carcinoma erythroleukemia, liver, colon carcinoma, among other tissues. Regarding which tissues have PDCD6IP expression, here are a few articles citing expression in various tissues:
Brain, Pubmed ID: 10718198
Cervix carcinoma, Pubmed ID: 18669648, 20068231
Cervix carcinoma, and Erythroleukemia, Pubmed ID: 23186163
Colon carcinoma, Pubmed ID: 24129315
Liver, Pubmed ID: 24275569
Lymph, and Testis, Pubmed ID: 15489334
Melanoma, Pubmed ID: 17081065
Placenta, Pubmed ID: 14702039

Boster Scientific Support

Answered: 2015-11-04

Order DetailsPrice
M01751-1

100μg

$370
M01751-1-10ug

10μg sample (liquid)

$99
M01751-1-Biotin

100 μg Biotin conjugated

$570
M01751-1-Cy3

100 μg Cy3 conjugated

$570
M01751-1-Dylight488

100 μg Dylight488 conjugated

$570
M01751-1-Dylight550

100 μg Dylight550 conjugated

$570
M01751-1-Dylight594

100 μg Dylight594 conjugated

$570
M01751-1-FITC

100 μg FITC conjugated

$570
M01751-1-HRP

100 μg HRP conjugated

$570
M01751-1-APC

100 μg APC conjugated

$670
M01751-1-PE

100 μg PE conjugated

$670
M01751-1-carrier-free

Carrier Free

$370

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