Anti-ALIX/PDCD6IP Antibody Picoband®

Alix antibody

Boster Bio Anti-ALIX/PDCD6IP Antibody Picoband® catalog # PB9770. Tested in IF, IHC, ICC, WB applications. This antibody reacts with Human, Mouse, Rat. The brand Picoband indicates this is a premium antibody that guarantees superior quality, high affinity, and strong signals with minimal background in Western blot applications. Only our best-performing antibodies are designated as Picoband, ensuring unmatched performance. Cited in 1 publication(s).

Product Info Summary

SKU: PB9770
Size: 100 μg/vial
Reactive Species: Human, Mouse, Rat
Host: Rabbit
Application: IF, IHC, ICC, WB

Customers Who Bought This Also Bought

Product Name

Anti-ALIX/PDCD6IP Antibody Picoband®

View all Alix Antibodies

SKU/Catalog Number

PB9770

Size

100 μg/vial

Form

Lyophilized

Description

Boster Bio Anti-ALIX/PDCD6IP Antibody Picoband® catalog # PB9770. Tested in IF, IHC, ICC, WB applications. This antibody reacts with Human, Mouse, Rat. The brand Picoband indicates this is a premium antibody that guarantees superior quality, high affinity, and strong signals with minimal background in Western blot applications. Only our best-performing antibodies are designated as Picoband, ensuring unmatched performance.

Storage & Handling

Store at -20˚C for one year from date of receipt. After reconstitution, at 4˚C for one month. It can also be aliquotted and stored frozen at -20˚C for six months. Avoid repeated freeze-thaw cycles.

Cite This Product

Anti-ALIX/PDCD6IP Antibody Picoband® (Boster Biological Technology, Pleasanton CA, USA, Catalog # PB9770)

Host

Rabbit

Contents

Each vial contains 5mg BSA, 0.9mg NaCl, 0.2mg Na2HPO4, 0.05mg NaN3.

Clonality

Polyclonal

Isotype

Rabbit IgG

Immunogen

E.coli-derived human ALIX recombinant protein (Position: A2-D330). Human ALIX shares 96.7% and 95.2% amino acid (aa) sequence identity with mouse and rat ALIX, respectively.

*Blocking peptide can be purchased. Costs vary based on immunogen length. Contact us for pricing.

Cross-reactivity

No cross-reactivity with other proteins.

Reactive Species

PB9770 is reactive to PDCD6IP in Human, Mouse, Rat

Reconstitution

Add 0.2ml of distilled water will yield a concentration of 500ug/ml.

Observed Molecular Weight

96 kDa

Calculated molecular weight

96023 MW

Background of Alix

Programmed cell death 6-interacting protein is a protein that in humans is encoded by the PDCD6IP gene. This gene encodes a protein that functions within the ESCRT pathway in the abscission stage of cytokinesis, in intralumenal endosomal vesicle formation, and in enveloped virus budding. Studies using mouse cells have shown that overexpression of this protein can block apoptosis. In addition, the product of this gene binds to the product of the PDCD6 gene, a protein required for apoptosis, in a calcium-dependent manner. This gene product also binds to endophilins, proteins that regulate membrane shape during endocytosis. Overexpression of this gene product and endophilins results in cytoplasmic vacuolization, which may be partly responsible for the protection against cell death. Several alternatively spliced transcript variants encoding different isoforms have been found for this gene.

Antibody Validation

Boster validates all antibodies on WB, IHC, ICC, Immunofluorescence, and ELISA with known positive control and negative samples to ensure specificity and high affinity, including thorough antibody incubations.

View more details

Applications

PB9770 is guaranteed for IF, IHC, ICC, WB Boster Guarantee

Assay Dilutions Recommendation

The recommendations below provide a starting point for assay optimization. The actual working concentration varies and should be decided by the user.

Western blot, 0.1-0.5μg/ml, Human, Mouse, Rat
Immunohistochemistry (Paraffin-embedded Section), 0.5-1μg/ml, Human, Mouse, Rat, By Heat Immunocytochemistry/Immunofluorescence, 5 μg/ml, Human

Positive Control

WB: human Hela whole cell, human HepG2 whole cell, human PANC-1 whole cell, rat liver tissue, rat RH35 whole cell, mouse liver tissue, mouse NIH/3T3 whole cell
IHC: rat liver tissue, huamn lung tissue, huamn oesophagus squama tissue, huamn placenta tissue, mouse intestine tissue
ICC/IF: T-47D cell

Validation Images & Assay Conditions

Gene/Protein Information For PDCD6IP (Source: Uniprot.org, NCBI)

Gene Name

PDCD6IP

Full Name

Programmed cell death 6-interacting protein

Weight

96023 MW

Alternative Names

Programmed cell death 6-interacting protein;PDCD6-interacting protein;ALG-2-interacting protein 1;ALG-2-interacting protein X;Hp95;PDCD6IP;AIP1, ALIX, KIAA1375; PDCD6IP AIP1, ALIX, DRIP4, HP95 programmed cell death 6 interacting protein programmed cell death 6-interacting protein|ALG-2 interacting protein 1|ALG-2-interacting protein X|PDCD6-interacting protein|apoptosis-linked gene 2-interacting protein X|dopamine receptor interacting protein 4

*If product is indicated to react with multiple species, protein info is based on the gene entry specified above in "Species".

For more info on PDCD6IP, check out the PDCD6IP Infographic

PDCD6IP infographic

We have 30,000+ of these available, one for each gene! Check them out.

In this infographic, you will see the following information for PDCD6IP: database IDs, superfamily, protein function, synonyms, molecular weight, chromosomal locations, tissues of expression, subcellular locations, post-translational modifications, and related diseases, research areas & pathways. If you want to see more information included, or would like to contribute to it and be acknowledged, please contact [email protected].

Hello CJ!

PB9770 has been cited in 1 publications:

*The publications in this section are manually curated by our staff scientists. They may differ from Bioz's machine gathered results. Both are accurate. If you find a publication citing this product but is missing from this list, please let us know we will issue you a thank-you coupon.

Cao G,Meng X, Han X,Li J.Exosomes derived from circRNA Rtn4-modified BMSCs attenuate TNF-α-induced cytotoxicity and apoptosis in murine MC3T3-E1 cells by sponging miR-146a.Biosci Rep.2020 May 29;40(5): BSR20193436.doi:10.1042/BSR20193436.PMID:32400849;PMC
Species: Mouse
PB9770 usage in article: APP:WB, SAMPLE:MC3T3-E1 CELLS, DILUTION:NA

Have you used Anti-ALIX/PDCD6IP Antibody Picoband®?

Submit a review and receive an Amazon gift card.

  • $30 for a review with an image

0 Reviews For Anti-ALIX/PDCD6IP Antibody Picoband®

Have a question?

Find answers in Q&As, reviews.

Can't find your answer?

Submit your question

16 Customer Q&As for Anti-ALIX/PDCD6IP Antibody Picoband®

Question

My question regarding product PB9770, anti-ALIX/PDCD6IP antibody. I was wondering if it would be possible to conjugate this antibody with biotin. I would need it to be without BSA or sodium azide. I am planning on using a buffer exchange of sodium azide with PBS only. Would there be problems for me to conjugate the antibody and store it in -20 degrees in small aliquots?

Verified Customer

Verified customer

Asked: 2020-03-04

Answer

It is not recommended storing this antibody with PBS buffer only in -20 degrees. If you want to store it in -20 degrees it is best to add some cryoprotectant like glycerol. If you want carrier free PB9770 anti-ALIX/PDCD6IP antibody, we can provide it to you in a special formula with trehalose and/or glycerol. These molecules will not interfere with conjugation chemistry and provide a good level of protection for the antibody from degradation. Please be sure to specify this in your purchase order.

Boster Scientific Support

Answered: 2020-03-04

Question

I see that the anti-ALIX/PDCD6IP antibody PB9770 works with WB, what is the protocol used to produce the result images on the product page?

Verified Customer

Verified customer

Asked: 2020-02-26

Answer

You can find protocols for WB on the "support/technical resources" section of our navigation menu. If you have any further questions, please send an email to [email protected]

Boster Scientific Support

Answered: 2020-02-26

Question

Is this PB9770 anti-ALIX/PDCD6IP antibody reactive to the isotypes of PDCD6IP?

R. Krishna

Verified customer

Asked: 2020-01-16

Answer

The immunogen of PB9770 anti-ALIX/PDCD6IP antibody is E.coli-derived human ALIX recombinant protein (Position: A2-D330). Human ALIX shares 96.7% and 95.2% amino acid (aa) sequence identity with mouse and rat ALIX, respectively. Could you tell me which isotype you are interested in so I can help see if the immunogen is part of this isotype?

Boster Scientific Support

Answered: 2020-01-16

Question

We are currently using anti-ALIX/PDCD6IP antibody PB9770 for human tissue, and we are well pleased with the WB results. The species of reactivity given in the datasheet says human, mouse, rat. Is it likely that the antibody can work on goat tissues as well?

Verified Customer

Verified customer

Asked: 2020-01-16

Answer

The anti-ALIX/PDCD6IP antibody (PB9770) has not been tested for cross reactivity specifically with goat tissues, though there is a good chance of cross reactivity. We have an innovator award program that if you test this antibody and show it works in goat you can get your next antibody for free. Please contact me if I can help you with anything.

Boster Scientific Support

Answered: 2020-01-16

Question

Thank you for helping with my inquiry over the phone. Here are the WB image, lot number and protocol we used for colon carcinoma using anti-ALIX/PDCD6IP antibody PB9770. Let me know if you need anything else.

Verified Customer

Verified customer

Asked: 2019-10-14

Answer

I appreciate the data. You have provided everything we needed. Our lab team are working to resolve your inquiry as quickly as possible, and we appreciate your patience and understanding! Please let me know if there is anything you need in the meantime.

Boster Scientific Support

Answered: 2019-10-14

Question

Do you have a BSA free version of anti-ALIX/PDCD6IP antibody PB9770 available?

Verified Customer

Verified customer

Asked: 2019-10-03

Answer

Thank you for your recent telephone inquiry. I can confirm that some lots of this anti-ALIX/PDCD6IP antibody PB9770 are BSA free. For now, these lots are available and we can make a BSA free formula for you free of charge. It will take 3 extra days to prepare. If you require this antibody BSA free again in future, please do not hesitate to contact me and I will be pleased to check which lots we have in stock that are BSA free.

Boster Scientific Support

Answered: 2019-10-03

Question

I was wanting to use your anti-ALIX/PDCD6IP antibody for WB for human colon carcinoma on frozen tissues, but I want to know if it has been tested for this particular application. Has this antibody been tested and is this antibody a good choice for human colon carcinoma identification?

Verified Customer

Verified customer

Asked: 2019-08-09

Answer

It shows on the product datasheet, PB9770 anti-ALIX/PDCD6IP antibody has been validated for IHC, WB on human, mouse, rat tissues. We have an innovator award program that if you test this antibody and show it works in human colon carcinoma in IHC-frozen, you can get your next antibody for free.

Boster Scientific Support

Answered: 2019-08-09

Question

Will anti-ALIX/PDCD6IP antibody PB9770 work for WB with colon carcinoma?

Verified Customer

Verified customer

Asked: 2019-07-03

Answer

According to the expression profile of colon carcinoma, PDCD6IP is highly expressed in colon carcinoma. So, it is likely that anti-ALIX/PDCD6IP antibody PB9770 will work for WB with colon carcinoma.

Boster Scientific Support

Answered: 2019-07-03

Question

We were well pleased with the WB result of your anti-ALIX/PDCD6IP antibody. However we have seen positive staining in brain cytosol using this antibody. Is that expected? Could you tell me where is PDCD6IP supposed to be expressed?

Verified Customer

Verified customer

Asked: 2019-05-30

Answer

From what I have seen in literature, brain does express PDCD6IP. Generally PDCD6IP expresses in cytoplasm, cytosol. Regarding which tissues have PDCD6IP expression, here are a few articles citing expression in various tissues:
Brain, Pubmed ID: 10718198
Cervix carcinoma, Pubmed ID: 18669648, 20068231
Cervix carcinoma, and Erythroleukemia, Pubmed ID: 23186163
Colon carcinoma, Pubmed ID: 24129315
Liver, Pubmed ID: 24275569
Lymph, and Testis, Pubmed ID: 15489334
Melanoma, Pubmed ID: 17081065
Placenta, Pubmed ID: 14702039

Boster Scientific Support

Answered: 2019-05-30

Question

Here is the WB image, lot number and protocol we used for colon carcinoma using anti-ALIX/PDCD6IP antibody PB9770. Please let me know if you require anything else.

Verified Customer

Verified customer

Asked: 2019-05-21

Answer

Thank you very much for the data. Our lab team are working to resolve this as quickly as possible, and we appreciate your patience and understanding! You have provided everything we needed. Please let me know if there is anything you need in the meantime.

Boster Scientific Support

Answered: 2019-05-21

Question

you antibody using your anti-ALIX/PDCD6IP antibody for protein transport studies. Has this antibody been tested with western blotting on nih3t3 whole cell lysates? We would like to see some validation images before ordering.

Verified Customer

Verified customer

Asked: 2018-07-23

Answer

We appreciate your inquiry. This PB9770 anti-ALIX/PDCD6IP antibody is tested on rat liver tissue, brain tissue, testis tissue, mouse liver tissue, spleen tissue, nih3t3 whole cell lysates, human hela, hela whole cell lysates. It is guaranteed to work for IHC, WB in human, mouse, rat. Our Boster guarantee will cover your intended experiment even if the sample type has not been be directly tested.

Boster Scientific Support

Answered: 2018-07-23

Question

Is a blocking peptide available for product anti-ALIX/PDCD6IP antibody (PB9770)?

S. Banerjee

Verified customer

Asked: 2018-04-05

Answer

We do provide the blocking peptide for product anti-ALIX/PDCD6IP antibody (PB9770). If you would like to place an order for it please contact [email protected] and make a special request.

Boster Scientific Support

Answered: 2018-04-05

Question

Will PB9770 anti-ALIX/PDCD6IP antibody work on parafin embedded sections? If so, which fixation method do you recommend we use (PFA, paraformaldehyde, other)?

J. Baker

Verified customer

Asked: 2016-02-22

Answer

You can see on the product datasheet, PB9770 anti-ALIX/PDCD6IP antibody as been tested on WB. It is best to use PFA for fixation because it has better tissue penetration ability. PFA needs to be prepared fresh before use. Long term stored PFA turns into formalin, as the PFA molecules congregate and become formalin.

Boster Scientific Support

Answered: 2016-02-22

Question

We are interested in to test anti-ALIX/PDCD6IP antibody PB9770 on human colon carcinoma for research purposes, then I may be interested in using anti-ALIX/PDCD6IP antibody PB9770 for diagnostic purposes as well. Is the antibody suitable for diagnostic purposes?

T. Parker

Verified customer

Asked: 2015-01-07

Answer

The products we sell, including anti-ALIX/PDCD6IP antibody PB9770, are only intended for research use. They would not be suitable for use in diagnostic work. If you have the means to develop a product into diagnostic use, and are interested in collaborating with us and develop our product into an IVD product, please contact us for more discussions.

Boster Scientific Support

Answered: 2015-01-07

Question

Our lab used your anti-ALIX/PDCD6IP antibody for IHC on brain last year. I am using rat, and I plan to use the antibody for WB next. I am interested in examining brain as well as melanoma in our next experiment. Could give a recommendation on which antibody would work the best for WB?

K. Wu

Verified customer

Asked: 2013-08-02

Answer

I have checked the website and datasheets of our anti-ALIX/PDCD6IP antibody and it appears that PB9770 has been tested on rat in both IHC and WB. Thus PB9770 should work for your application. Our Boster satisfaction guarantee will cover this product for WB in rat even if the specific tissue type has not been validated. We do have a comprehensive range of products for WB detection and you can check out our website bosterbio.com to find out more information about them.

Boster Scientific Support

Answered: 2013-08-02

Question

We have seen staining in mouse cervix carcinoma. Are there any suggestions? Is anti-ALIX/PDCD6IP antibody supposed to stain cervix carcinoma positively?

J. Li

Verified customer

Asked: 2013-04-22

Answer

According to literature cervix carcinoma does express PDCD6IP. According to Uniprot.org, PDCD6IP is expressed in corpus callosum, lymph testis, osteosarcoma, placenta, brain, melanoma, cervix carcinoma, cervix carcinoma erythroleukemia, liver, colon carcinoma, among other tissues. Regarding which tissues have PDCD6IP expression, here are a few articles citing expression in various tissues:
Brain, Pubmed ID: 10718198
Cervix carcinoma, Pubmed ID: 18669648, 20068231
Cervix carcinoma, and Erythroleukemia, Pubmed ID: 23186163
Colon carcinoma, Pubmed ID: 24129315
Liver, Pubmed ID: 24275569
Lymph, and Testis, Pubmed ID: 15489334
Melanoma, Pubmed ID: 17081065
Placenta, Pubmed ID: 14702039

Boster Scientific Support

Answered: 2013-04-22

Order DetailsPrice
PB9770

100μg

$370
PB9770-10ug

10μg sample (liquid)

$99
PB9770-Biotin

100 μg Biotin conjugated

$570
PB9770-Cy3

100 μg Cy3 conjugated

$570
PB9770-Dylight488

100 μg Dylight488 conjugated

$570
PB9770-Dylight550

100 μg Dylight550 conjugated

$570
PB9770-Dylight594

100 μg Dylight594 conjugated

$570
PB9770-FITC

100 μg FITC conjugated

$570
PB9770-HRP

100 μg HRP conjugated

$570
PB9770-APC

100 μg APC conjugated

$670
PB9770-PE

100 μg PE conjugated

$670
PB9770-iFluor647

100 μg iFluor647 conjugated

$670
PB9770-carrier-free

Carrier Free

$370

More conjugates/formats

Free Secondary Antibody

Choose an option to show lead time.
Get A Quote
In stock
Order Product
PB9770
Buy one primary antibody get one 0.5ml HRP or Biotin secondary antibody for free.
*Sample sizes are prepared on demand and will take extra lead time. (cannot be conjugated)
$370.00

Troubleshooting

troubleshooting-box-image

Download troubleshooting handbooks for IHC, Western blot and ELISA for FREE.

Download Free PDFs Now

Boster Guarantee

Guaranteed product quality

Guaranteed product quality

We promise all of our products perform as described in datasheets.