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Product Info Summary
| SKU: | A04039-1 |
|---|---|
| Size: | 100μl |
| Reactive Species: | Human, Mouse, Rat |
| Host: | Rabbit |
| Application: | WB |
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Product info
Product Name
Anti-Tom20 TOMM20 Antibody
SKU/Catalog Number
A04039-1
Size
100μl
Form
Liquid
Description
Boster Bio Anti-Tom20 TOMM20 Antibody catalog # A04039-1. Tested in WB applications. This antibody reacts with Human, Mouse, Rat.
Storage & Handling
Store at -20°C for one year. For short term storage and frequent use, store at 4°C for up to one month. Avoid repeated freeze-thaw cycles.
Cite This Product
Anti-Tom20 TOMM20 Antibody (Boster Biological Technology, Pleasanton CA, USA, Catalog # A04039-1)
Host
Rabbit
Contents
Liquid in PBS containing 50% glycerol, 0.5% BSA and 0.02% sodium azide.
Clonality
Polyclonal
Isotype
IgG
Immunogen
Synthesized peptide derived from the Internal region of human Tom20. at AA rangle: 30-110
*Blocking peptide can be purchased. Costs vary based on immunogen length. Contact us for pricing.
Reactive Species
A04039-1 is reactive to TOMM20 in Human, Mouse, Rat
Applications
A04039-1 is guaranteed for WB Boster Guarantee
Observed Molecular Weight
39 kDa
Calculated molecular weight
16298 MW
Background of TOMM20
Transcriptional repressor which binds neuron-restrictive silencer element (NRSE) and represses neuronal gene transcription in non-neuronal cells. Restricts the expression of neuronal genes by associating with two distinct corepressors, mSin3 and CoREST, which in turn recruit histone deacetylase to the promoters of REST-regulated genes. Mediates repression by recruiting the BHC complex at RE1/NRSE sites which acts by deacetylating and demethylating specific sites on histones, thereby acting as a chromatin modifier.
Chong J.A.et.al. (1995)Cell 80:949-957
Schoenherr C.J.et.al.(1995)Science 267:1360-1363
Scholl T.et.al.(1996)J. Immunol. 156:1448-1457
Lunyak V.V.et.al. (2002)Science 298:1747-1752
Antibody Validation
Boster validates all antibodies on WB, IHC, ICC, Immunofluorescence, and ELISA with known positive control and negative samples to ensure specificity and high affinity, including thorough antibody incubations.
Assay dilution & Images
Assay Dilutions Recommendation
The recommendations below provide a starting point for assay optimization. The actual working concentration varies and should be decided by the user.
WB, 1:500-1:2000
Validation Images & Assay Conditions
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Western Blot (WB) analysis of specific cells using Tom20 Polyclonal antibody.
Protein Target Info & Infographic
Gene/Protein Information For TOMM20 (Source: Uniprot.org, NCBI)
Gene Name
TOMM20
Full Name
Mitochondrial import receptor subunit TOM20 homolog
Weight
16298 MW
Superfamily
Tom20 family
Alternative Names
KIAA0016Outer mitochondrial membrane receptor Tom20; MAS20; MGC117367; Mitochondrial 20 kDa outer membrane protein; mitochondrial import receptor subunit TOM20 homolog; MOM19; TOM20; translocase of outer mitochondrial membrane 20 homolog (yeast); translocase of outer mitochondrial membrane 20 homolog type II TOMM20 MAS20, MOM19, TOM20 translocase of outer mitochondrial membrane 20 mitochondrial import receptor subunit TOM20 homolog|mitochondrial 20 kDa outer membrane protein|outer mitochondrial membrane receptor Tom20|translocase of outer mitochondrial membrane 20 homolog type II
*If product is indicated to react with multiple species, protein info is based on the gene entry specified above in "Species".For more info on TOMM20, check out the TOMM20 Infographic
We have 30,000+ of these available, one for each gene! Check them out.
In this infographic, you will see the following information for TOMM20: database IDs, superfamily, protein function, synonyms, molecular weight, chromosomal locations, tissues of expression, subcellular locations, post-translational modifications, and related diseases, research areas & pathways. If you want to see more information included, or would like to contribute to it and be acknowledged, please contact [email protected].
Specific Publications For Anti-Tom20 TOMM20 Antibody (A04039-1)
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No publications found for A04039-1
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Customer Reviews
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Customer Q&As
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12 Customer Q&As for Anti-Tom20 TOMM20 Antibody
Question
Is a blocking peptide available for product anti-Tom20 antibody (A04039-1)?
Verified Customer
Verified customer
Asked: 2020-03-11
Answer
We do provide the blocking peptide for product anti-Tom20 antibody (A04039-1). If you would like to place an order for it please contact [email protected] and make a special request.
Boster Scientific Support
Answered: 2020-03-11
Question
Is this A04039-1 anti-Tom20 antibody reactive to the isotypes of TOMM20?
Verified Customer
Verified customer
Asked: 2020-02-20
Answer
The immunogen of A04039-1 anti-Tom20 antibody is Synthesized peptide derived from the Internal region of human Tom20. at AA rangle: 30-110. Could you tell me which isotype you are interested in so I can help see if the immunogen is part of this isotype?
Boster Scientific Support
Answered: 2020-02-20
Question
Do you have a BSA free version of anti-Tom20 antibody A04039-1 available?
Verified Customer
Verified customer
Asked: 2019-10-10
Answer
Thanks for your recent telephone inquiry. I can confirm that some lots of this anti-Tom20 antibody A04039-1 are BSA free. For now, these lots are available and we can make a BSA free formula for you free of charge. It will take 3 extra days to prepare. If you require this antibody BSA free again in future, please do not hesitate to contact me and I will be pleased to check which lots we have in stock that are BSA free.
Boster Scientific Support
Answered: 2019-10-10
Question
Thank you for helping with my inquiry over the phone. Here are the WB image, lot number and protocol we used for liver using anti-Tom20 antibody A04039-1. Let me know if you need anything else.
Verified Customer
Verified customer
Asked: 2019-09-11
Answer
Thank you for the data. You have provided everything we needed. Our lab team are working to resolve your inquiry as quickly as possible, and we appreciate your patience and understanding! Please let me know if there is anything you need in the meantime.
Boster Scientific Support
Answered: 2019-09-11
Question
I see that the anti-Tom20 antibody A04039-1 works with WB, what is the protocol used to produce the result images on the product page?
Verified Customer
Verified customer
Asked: 2019-08-01
Answer
You can find protocols for WB on the "support/technical resources" section of our navigation menu. If you have any further questions, please send an email to [email protected]
Boster Scientific Support
Answered: 2019-08-01
Question
I was wanting to use your anti-Tom20 antibody for WB for rat liver on frozen tissues, but I want to know if it has been tested for this particular application. Has this antibody been tested and is this antibody a good choice for rat liver identification?
Verified Customer
Verified customer
Asked: 2019-07-09
Answer
As indicated on the product datasheet, A04039-1 anti-Tom20 antibody has been validated for WB on human, mouse, rat tissues. We have an innovator award program that if you test this antibody and show it works in rat liver in IHC-frozen, you can get your next antibody for free.
Boster Scientific Support
Answered: 2019-07-09
Question
Does anti-Tom20 antibody A04039-1 work on pig WB with brain?
Verified Customer
Verified customer
Asked: 2019-06-28
Answer
Our lab technicians have not tested anti-Tom20 antibody A04039-1 on pig. You can run a BLAST between pig and the immunogen sequence of anti-Tom20 antibody A04039-1 to see if they may cross-react. If the sequence homology is close, then you can perform a pilot test. Keep in mind that since we have not validated pig samples, this use of the antibody is not covered by our guarantee. However we have an innovator award program that if you test this antibody and show it works in pig brain in WB, you can get your next antibody for free.
Boster Scientific Support
Answered: 2019-06-28
Question
I was wanting to use to test anti-Tom20 antibody A04039-1 on rat liver for research purposes, then I may be interested in using anti-Tom20 antibody A04039-1 for diagnostic purposes as well. Is the antibody suitable for diagnostic purposes?
Verified Customer
Verified customer
Asked: 2018-12-14
Answer
The products we sell, including anti-Tom20 antibody A04039-1, are only intended for research use. They would not be suitable for use in diagnostic work. If you have the means to develop a product into diagnostic use, and are interested in collaborating with us and develop our product into an IVD product, please contact us for more discussions.
Boster Scientific Support
Answered: 2018-12-14
Question
Can you help my question with product A04039-1, anti-Tom20 antibody. I was wondering if it would be possible to conjugate this antibody with biotin. I would need it to be without BSA or sodium azide. I am planning on using a buffer exchange of sodium azide with PBS only. Would there be problems for me to conjugate the antibody and store it in -20 degrees in small aliquots?
Verified Customer
Verified customer
Asked: 2017-11-02
Answer
We suggest not storing this antibody with PBS buffer only in -20 degrees. If you want to store it in -20 degrees it is best to add some cryoprotectant like glycerol. If you want carrier free A04039-1 anti-Tom20 antibody, we can provide it to you in a special formula with trehalose and/or glycerol. These molecules will not interfere with conjugation chemistry and provide a good level of protection for the antibody from degradation. Please be sure to specify this in your purchase order.
Boster Scientific Support
Answered: 2017-11-02
Question
I have attached the WB image, lot number and protocol we used for liver using anti-Tom20 antibody A04039-1. Please let me know if you require anything else.
W. Parker
Verified customer
Asked: 2017-07-07
Answer
Thank you very much for the data. Our lab team are working to resolve this as quickly as possible, and we appreciate your patience and understanding! You have provided everything we needed. Please let me know if there is anything you need in the meantime.
Boster Scientific Support
Answered: 2017-07-07
Question
Would A04039-1 anti-Tom20 antibody work on parafin embedded sections? If so, which fixation method do you recommend we use (PFA, paraformaldehyde, other)?
L. Li
Verified customer
Asked: 2014-07-31
Answer
It shows on the product datasheet, A04039-1 anti-Tom20 antibody as been tested on WB. It is best to use PFA for fixation because it has better tissue penetration ability. PFA needs to be prepared fresh before use. Long term stored PFA turns into formalin, as the PFA molecules congregate and become formalin.
Boster Scientific Support
Answered: 2014-07-31
Question
Does anti-Tom20 antibody A04039-1 work for WB with liver?
M. Dhar
Verified customer
Asked: 2013-08-02
Answer
According to the expression profile of liver, TOMM20 is highly expressed in liver. So, it is likely that anti-Tom20 antibody A04039-1 will work for WB with liver.
Boster Scientific Support
Answered: 2013-08-02
