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Product Info Summary
| SKU: | A00010Y1221Y1222-1 |
|---|---|
| Size: | 100ul |
| Reactive Species: | Human, Mouse, Rat |
| Host: | Rabbit |
| Application: | ELISA, IP, IHC, WB |
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Product info
Product Name
Anti-Phospho-Neu (Y1221/Y1222) ERBB2 Antibody
View all ErbB2/Her2 Antibodies
SKU/Catalog Number
A00010Y1221Y1222-1
Size
100ul
Form
Liquid
Description
Boster Bio Anti-Phospho-Neu (Y1221/Y1222) ERBB2 Antibody catalog # A00010Y1221Y1222-1. Tested in ELISA, IP, IHC, WB applications. This antibody reacts with Human, Mouse, Rat.
Storage & Handling
Store at -20°C for one year. For short term storage and frequent use, store at 4°C for up to one month. Avoid repeated freeze-thaw cycles.
Cite This Product
Anti-Phospho-Neu (Y1221/Y1222) ERBB2 Antibody (Boster Biological Technology, Pleasanton CA, USA, Catalog # A00010Y1221Y1222-1)
Host
Rabbit
Contents
Liquid in PBS containing 50% glycerol, 0.5% BSA and 0.02% sodium azide.
Clonality
Polyclonal
Isotype
IgG
Immunogen
The antiserum was produced against synthesized peptide derived from human HER2 around the phosphorylation site of Tyr1221/Tyr1222. AA range:1191-1240
*Blocking peptide can be purchased. Costs vary based on immunogen length. Contact us for pricing.
Reactive Species
A00010Y1221Y1222-1 is reactive to ERBB2 in Human, Mouse, Rat
Reconstitution
Calculated molecular weight
137910 MW
Antibody Validation
Boster validates all antibodies on WB, IHC, ICC, Immunofluorescence, and ELISA with known positive control and negative samples to ensure specificity and high affinity, including thorough antibody incubations.
Application & Images
Applications
A00010Y1221Y1222-1 is guaranteed for ELISA, IP, IHC, WB Boster Guarantee
Assay Dilutions Recommendation
The recommendations below provide a starting point for assay optimization. The actual working concentration varies and should be decided by the user.
WB 1:500-1:2000
IHC 1:100-1:300
IP 1:200-500
ELISA 1:20000
Validation Images & Assay Conditions
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Western Blot analysis of various cells using Phospho-Neu (Y1221/Y1222) polyclonal antibody
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Immunohistochemistry analysis of paraffin-embedded human breast carcinoma, using HER2 (Phospho-Tyr1221/Tyr1222) antibody. The picture on the right is blocked with the phospho peptide.
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Western blot analysis of lysates from SK-OV3 cells treated with EGF, using HER2 (Phospho-Tyr1221/Tyr1222) antibody. The lane on the right is blocked with the phospho peptide.
Protein Target Info & Infographic
Gene/Protein Information For ERBB2 (Source: Uniprot.org, NCBI)
Gene Name
ERBB2
Full Name
Receptor tyrosine-protein kinase erbB-2
Weight
137910 MW
Superfamily
protein kinase superfamily
Alternative Names
Receptor tyrosine-protein kinase erbB-2;2.7.10.1;Metastatic lymph node gene 19 protein;MLN 19;Proto-oncogene Neu;Proto-oncogene c-ErbB-2;Tyrosine kinase-type cell surface receptor HER2;p185erbB2;CD340;ERBB2;HER2, MLN19, NEU, NGL; ERBB2 CD340, HER-2, HER-2/neu, HER2, MLN 19, NEU, NGL, TKR1 erb-b2 receptor tyrosine kinase 2 receptor tyrosine-protein kinase erbB-2|c-erb B2/neu protein|herstatin|human epidermal growth factor receptor 2|metastatic lymph node gene 19 protein|neuro/glioblastoma derived oncogene homolog|neuroblastoma/glioblastoma derived oncogene homolog|proto-oncogene Neu|proto-oncogene c-ErbB-2|tyrosine kinase-type cell surface receptor HER2|v-erb-b2 avian erythroblastic leukemia viral oncogene homolog 2|v-erb-b2 avian erythroblastic leukemia viral oncoprotein 2|v-erb-b2 erythroblastic leukemia viral oncogene homolog 2, neuro/glioblastoma derived oncogene homolog
*If product is indicated to react with multiple species, protein info is based on the gene entry specified above in "Species".For more info on ERBB2, check out the ERBB2 Infographic
We have 30,000+ of these available, one for each gene! Check them out.
In this infographic, you will see the following information for ERBB2: database IDs, superfamily, protein function, synonyms, molecular weight, chromosomal locations, tissues of expression, subcellular locations, post-translational modifications, and related diseases, research areas & pathways. If you want to see more information included, or would like to contribute to it and be acknowledged, please contact [email protected].
Specific Publications For Anti-Phospho-Neu (Y1221/Y1222) ERBB2 Antibody (A00010Y1221Y1222-1)
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