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Product Info Summary
| SKU: | PA1974 |
|---|---|
| Size: | 100 μg/vial |
| Reactive Species: | Mouse, Rat |
| Host: | Rabbit |
| Application: | WB |
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Product info
Product Name
Anti-TNF Receptor II/TNFRSF1B Antibody Picoband®
View all TNF RII/TNFRSF1B Antibodies
SKU/Catalog Number
PA1974
Size
100 μg/vial
Form
Lyophilized
Description
Boster Bio Anti-TNF Receptor II/TNFRSF1B Antibody catalog # PA1974. Tested in WB applications. This antibody reacts with Mouse, Rat. The brand Picoband indicates this is a premium antibody that guarantees superior quality, high affinity, and strong signals with minimal background in Western blot applications. Only our best-performing antibodies are designated as Picoband, ensuring unmatched performance.
Storage & Handling
Store at -20˚C for one year from date of receipt. After reconstitution, at 4˚C for one month. It can also be aliquotted and stored frozen at -20˚C for six months. Avoid repeated freeze-thaw cycles.
Cite This Product
Anti-TNF Receptor II/TNFRSF1B Antibody Picoband® (Boster Biological Technology, Pleasanton CA, USA, Catalog # PA1974)
Host
Rabbit
Contents
Each vial contains 5mg BSA, 0.9mg NaCl, 0.2mg Na2HPO4, 0.05mg Thimerosal, 0.05mg NaN3.
Clonality
Polyclonal
Isotype
Rabbit IgG
Immunogen
A synthetic peptide corresponding to a sequence at the N-terminus of mouse TNF Receptor II, different from the related rat sequence by three amino acids.
*Blocking peptide can be purchased. Costs vary based on immunogen length. Contact us for pricing.
Cross-reactivity
No cross-reactivity with other proteins
Reactive Species
PA1974 is reactive to Tnfrsf1b in Mouse, Rat
Reconstitution
Add 0.2ml of distilled water will yield a concentration of 500ug/ml.
Observed Molecular Weight
150 kDa
Calculated molecular weight
50320 MW
Background of TNF RII/TNFRSF1B
TNFRSF1B (Tumor Necrosis Factor Receptor Subfamily Member 1B), also known as TNFR2 or TNFBR, is a protein that in humans is encoded by the TNFRSF1B gene. The protein encoded by this gene is a member of the Tumor necrosis factor receptor superfamily, which also contains TNFRSF1A. By linkage analysis, Santee and Owen Schaub (1996) confirmed the mapping of the TNFR2 gene to 1p36.3-p36.2. TNFBR (TNFR75) is the larger of the 2 TNF receptors. It is present on many cell types, especially those of myeloid origin, and is strongly expressed on stimulated T and B lymphocytes. Beltinger et al. (1996) noted that TNFR2 is the main TNF receptor found on circulating T cells and is the major mediator of autoregulatory apoptosis in CD8+ cells. TNFR2 may act with TNFR1 to kill nonlymphoid cells.
Antibody Validation
Boster validates all antibodies on WB, IHC, ICC, Immunofluorescence, and ELISA with known positive control and negative samples to ensure specificity and high affinity, including thorough antibody incubations.
Application & Images
Applications
PA1974 is guaranteed for WB Boster Guarantee
Assay Dilutions Recommendation
The recommendations below provide a starting point for assay optimization. The actual working concentration varies and should be decided by the user.
Western blot, 0.1-0.5μg/ml, Mouse, Rat
Positive Control
WB: Rat Spleen Tissue, Rat Brain Tissue, Mouse Brain Tissue, HEPA Cell,
Validation Images & Assay Conditions
Click image to see more details
Anti-TNF Receptor II antibody, PA1974, Western blotting
Lane 1: Rat Spleen Tissue Lysate
Lane 2: Rat Brain Tissue Lysate
Lane 3: Mouse Brain Tissue Lysate
Lane 4: HEPA Cell Lysate
Protein Target Info & Infographic
Gene/Protein Information For Tnfrsf1b (Source: Uniprot.org, NCBI)
Gene Name
Tnfrsf1b
Full Name
Tumor necrosis factor receptor superfamily member 1B
Weight
50320 MW
Alternative Names
Tumor necrosis factor receptor superfamily member 1B;Tumor necrosis factor receptor 2;TNF-R2;Tumor necrosis factor receptor type II;TNF-RII;TNFR-II;p75;p80 TNF-alpha receptor;CD120b;Tnfrsf1b;Tnfr-2, Tnfr2; TNFRSF1B CD120b, TBPII, TNF-R-II, TNF-R75, TNFBR, TNFR1B, TNFR2, TNFR80, p75, p75TNFR TNF receptor superfamily member 1B tumor necrosis factor receptor superfamily member 1B|TNF-R2|TNF-RII|p75 TNF receptor|p80 TNF-alpha receptor|soluble TNFR1B variant 1|tumor necrosis factor beta receptor|tumor necrosis factor binding protein 2|tumor necrosis factor receptor 2|tumor necrosis factor receptor type II
*If product is indicated to react with multiple species, protein info is based on the gene entry specified above in "Species".For more info on Tnfrsf1b, check out the Tnfrsf1b Infographic
We have 30,000+ of these available, one for each gene! Check them out.
In this infographic, you will see the following information for Tnfrsf1b: database IDs, superfamily, protein function, synonyms, molecular weight, chromosomal locations, tissues of expression, subcellular locations, post-translational modifications, and related diseases, research areas & pathways. If you want to see more information included, or would like to contribute to it and be acknowledged, please contact [email protected].
Specific Publications For Anti-TNF Receptor II/TNFRSF1B Antibody Picoband® (PA1974)
Hello CJ!
PA1974 has been cited in 2 publications:
*The publications in this section are manually curated by our staff scientists. They may differ from Bioz's machine gathered results. Both are accurate. If you find a publication citing this product but is missing from this list, please let us know we will issue you a thank-you coupon.
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Customer Reviews
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Customer Q&As
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6 Customer Q&As for Anti-TNF Receptor II/TNFRSF1B Antibody Picoband®
Question
Will PA1974 anti-TNF Receptor II/TNFRSF1B antibody work on parafin embedded sections? If so, which fixation method do you recommend we use (PFA, paraformaldehyde, other)?
Verified Customer
Verified customer
Asked: 2020-05-04
Answer
It shows on the product datasheet, PA1974 anti-TNF Receptor II/TNFRSF1B antibody as been tested on WB. It is best to use PFA for fixation because it has better tissue penetration ability. PFA needs to be prepared fresh before use. Long term stored PFA turns into formalin, as the PFA molecules congregate and become formalin.
Boster Scientific Support
Answered: 2020-05-04
Question
Thank you for helping with my inquiry over the phone. Here are the WB image, lot number and protocol we used for erythroleukemia using anti-TNF Receptor II/TNFRSF1B antibody PA1974. Let me know if you need anything else.
Verified Customer
Verified customer
Asked: 2019-12-13
Answer
I appreciate the data. You have provided everything we needed. Our lab team are working to resolve your inquiry as quickly as possible, and we appreciate your patience and understanding! Please let me know if there is anything you need in the meantime.
Boster Scientific Support
Answered: 2019-12-13
Question
Does anti-TNF Receptor II/TNFRSF1B antibody PA1974 work on zebrafish WB with urine?
Verified Customer
Verified customer
Asked: 2019-11-27
Answer
Our lab technicians have not tested anti-TNF Receptor II/TNFRSF1B antibody PA1974 on zebrafish. You can run a BLAST between zebrafish and the immunogen sequence of anti-TNF Receptor II/TNFRSF1B antibody PA1974 to see if they may cross-react. If the sequence homology is close, then you can perform a pilot test. Keep in mind that since we have not validated zebrafish samples, this use of the antibody is not covered by our guarantee. However we have an innovator award program that if you test this antibody and show it works in zebrafish urine in WB, you can get your next antibody for free.
Boster Scientific Support
Answered: 2019-11-27
Question
Is a blocking peptide available for product anti-TNF Receptor II/TNFRSF1B antibody (PA1974)?
Verified Customer
Verified customer
Asked: 2019-09-04
Answer
We do provide the blocking peptide for product anti-TNF Receptor II/TNFRSF1B antibody (PA1974). If you would like to place an order for it please contact [email protected] and make a special request.
Boster Scientific Support
Answered: 2019-09-04
Question
I see that the anti-TNF Receptor II/TNFRSF1B antibody PA1974 works with WB, what is the protocol used to produce the result images on the product page?
Verified Customer
Verified customer
Asked: 2019-03-21
Answer
You can find protocols for WB on the "support/technical resources" section of our navigation menu. If you have any further questions, please send an email to [email protected]
Boster Scientific Support
Answered: 2019-03-21
Question
Is this PA1974 anti-TNF Receptor II/TNFRSF1B antibody reactive to the isotypes of TNFRSF1B?
D. Anderson
Verified customer
Asked: 2016-01-25
Answer
The immunogen of PA1974 anti-TNF Receptor II/TNFRSF1B antibody is A synthetic peptide corresponding to a sequence at the N-terminus of mouse TNF Receptor II(38-55aa YECQISQEYYDRKAQMCC), different from the related rat sequence by three amino acids. Could you tell me which isotype you are interested in so I can help see if the immunogen is part of this isotype?
Boster Scientific Support
Answered: 2016-01-25
