Anti-HIPK2 Antibody

Product Name

Anti-HIPK2 Antibody

View all Hipk2 Antibodies

SKU/Catalog Number

A01371

Size

100ul

Form

Liquid

Description

Boster Bio Anti-HIPK2 Antibody catalog # A01371. Tested in WB applications. This antibody reacts with Human,Mouse,Rat.

Storage & Handling

Store at -20°C for one year. For short term storage and frequent use, store at 4°C for up to one month. Avoid repeated freeze-thaw cycles.

Cite This Product

Anti-HIPK2 Antibody (Boster Biological Technology, Pleasanton CA, USA, Catalog # A01371)

Host

Rabbit

Contents

Rabbit IgG, 1mg/ml in PBS with 0.02% sodium azide, 50% glycerol, pH7.2

Clonality

Polyclonal

Isotype

IgG

Immunogen

Synthesized peptide derived from part region of human protein

*Blocking peptide can be purchased. Costs vary based on immunogen length. Contact us for pricing.

Cross-reactivity

No cross reactivity with other proteins.

Reactive Species

A01371 is reactive to Hipk2 in Human, Mouse, Rat

Applications

A01371 is guaranteed for WB Boster Guarantee

Observed Molecular Weight

Calculated molecular weight

130966 MW

Background of Hipk2

Regulatory subunit for CDC7 which activates its kinase activity thereby playing a central role in DNA replication and cell proliferation. Required for progression of S phase. The complex CDC7-DBF4A selectively phosphorylates MCM2 subunit at 'Ser-40' and 'Ser-53' and then is involved in regulating the initiation of DNA replication during cell cycle.

Kumagai H., Mol. Cell. Biol. 19:5083-5095(1999).
Jiang W., EMBO J. 18:5703-5713(1999).
Ota T., Nat. Genet. 36:40-45(2004).

Antibody Validation

Boster validates all antibodies on WB, IHC, ICC, Immunofluorescence, and ELISA with known positive control and negative samples to ensure specificity and high affinity, including thorough antibody incubations.

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Assay Dilutions Recommendation

The recommendations below provide a starting point for assay optimization. The actual working concentration varies and should be decided by the user.

WB: 1:500-1:1000

Validation Images & Assay Conditions

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Figure 1. Western blotting validation for Anti-HIPK2 Antibody A01371

Western blot (WB) analysis of HIPK2 polyclonal antibody at 1:500 dilution
Lane1:Hela cell lysate
Lane2:sp2/0 cell lysate
Lane3:PC12 cell lysate
Electrophoresis was performed on a SDS-PAGE gel. To determine SDS-PAGE gel concentration

Gene/Protein Information For Hipk2 (Source: Uniprot.org, NCBI)

Alternative Names

DKFZp686K02111; EC 2.7.11.1; FLJ23711; hHIPk2; HIPK2; homeodomain interacting protein kinase 2; homeodomain-interacting protein kinase 2; PRO0593; Stank Hipk2|1110014O20Rik, B230339E18Rik, St, Stank|homeodomain interacting protein kinase 2|homeodomain-interacting protein kinase 2|nuclear body-associated kinase 1|sialophorin tail-associated nuclear serine/threonine-protein kinase

For more info on Hipk2, check out the Hipk2 Infographic

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In this infographic, you will see the following information for Hipk2: database IDs, superfamily, protein function, synonyms, molecular weight, chromosomal locations, tissues of expression, subcellular locations, post-translational modifications, and related diseases, research areas & pathways. If you want to see more information included, or would like to contribute to it and be acknowledged, please contact [email protected].

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