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Product Info Summary
| SKU: | PA1210 |
|---|---|
| Size: | 100 μg/vial |
| Reactive Species: | Human |
| Host: | Rabbit |
| Application: | IHC, WB |
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Product info
Product Name
Anti-TNF Receptor I/TNFRSF1A Antibody Picoband®
View all TNF RI/TNFRSF1A Antibodies
SKU/Catalog Number
PA1210
Size
100 μg/vial
Form
Lyophilized
Description
Boster Bio Anti-TNF Receptor I/TNFRSF1A Antibody catalog # PA1210. Tested in IHC, WB applications. This antibody reacts with Human. The brand Picoband indicates this is a premium antibody that guarantees superior quality, high affinity, and strong signals with minimal background in Western blot applications. Only our best-performing antibodies are designated as Picoband, ensuring unmatched performance.
Storage & Handling
Store at -20˚C for one year from date of receipt. After reconstitution, at 4˚C for one month. It can also be aliquotted and stored frozen at -20˚C for six months. Avoid repeated freeze-thaw cycles.
Cite This Product
Anti-TNF Receptor I/TNFRSF1A Antibody Picoband® (Boster Biological Technology, Pleasanton CA, USA, Catalog # PA1210)
Host
Rabbit
Contents
Each vial contains 5mg BSA, 0.9mg NaCl, 0.2mg Na2HPO4, 0.05mg Thimerosal, 0.05mg NaN3.
Clonality
Polyclonal
Isotype
Rabbit IgG
Immunogen
A synthetic peptide corresponding to a sequence in the middle region of human TNF Receptor I .
*Blocking peptide can be purchased. Costs vary based on immunogen length. Contact us for pricing.
Cross-reactivity
No cross-reactivity with other proteins
Reactive Species
PA1210 is reactive to TNFRSF1A in Human
Reconstitution
Add 0.2ml of distilled water will yield a concentration of 500ug/ml.
Observed Molecular Weight
50-60 kDa
Calculated molecular weight
50495 MW
Background of TNF RI/TNFRSF1A
Tumor necrosis factor receptor 1 (TNFR1), a potent cytokine, elicits a broad spectrum of biologic responses which are mediated by binding to a cell surface receptor. Its gene is located on 12p13.2. The coding region and the 3-prime untranslated region of TNFR1 are distributed over 10 exons. There are 2 different proteins that serve as major receptors for TNF-alpha, one associated with myeloid cells and one associated with epithelial cells. Additionally, TNFR1 associates with the MADD protein through a death domain-death domain interaction. MADD provides a physical link between TNFR1 and the induction of mitogen-activated protein (MAP) kinase (e.g., ERK2) activation and arachidonic acid release. TNFR1-induced apoptosis involves 2 sequential signaling complexes. Complex I, the initial plasma membrane-bound complex, consists of TNFR1, the adaptor TRADD, the kinase RIP1, and TRAF2 and rapidly signals activation of NF-kappa-B. In a second step, TRADD and RIP1 associate with FADD and caspase-8, forming a cytoplasmic complex, complex II.
Antibody Validation
Boster validates all antibodies on WB, IHC, ICC, Immunofluorescence, and ELISA with known positive control and negative samples to ensure specificity and high affinity, including thorough antibody incubations.
Application & Images
Applications
PA1210 is guaranteed for IHC, WB Boster Guarantee
Assay Dilutions Recommendation
The recommendations below provide a starting point for assay optimization. The actual working concentration varies and should be decided by the user.
Immunohistochemistry (Paraffin-embedded Section), 0.5-1μg/ml, Human, By Heat
Western blot, 0.1-0.5μg/ml, Human
Positive Control
WB: human SW579 whole cell
IHC: Human Mammary Tissue
Validation Images & Assay Conditions
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Anti-TNF Receptor I antibody, PA1210, IHC(P)
IHC(P): Human Mammary Tissue
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Figure 1. Western blot analysis of TNF Receptor I using anti-TNF Receptor I antibody (PA1210).
Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel) / 90V (Resolving gel) for 2-3 hours. The sample well of each lane was loaded with 50ug of sample under reducing conditions.
Lane 1: human SW579 whole cell lysates.
After Electrophoresis, proteins were transferred to a Nitrocellulose membrane at 150mA for 50-90 minutes. Blocked the membrane with 5% Non-fat Milk/ TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-TNF Receptor I antigen affinity purified polyclonal antibody (Catalog # PA1210) at 0.5 μg/mL overnight at 4°C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:10000 for 1.5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit (Catalog # EK1002) with Tanon 5200 system. A specific band was detected for TNF Receptor I at approximately 50-60KD. The expected band size for TNF Receptor I is at 50KD.
Protein Target Info & Infographic
Gene/Protein Information For TNFRSF1A (Source: Uniprot.org, NCBI)
Gene Name
TNFRSF1A
Full Name
Tumor necrosis factor receptor superfamily member 1A
Weight
50495 MW
Alternative Names
Tumor necrosis factor receptor superfamily member 1A;Tumor necrosis factor receptor 1;TNF-R1;Tumor necrosis factor receptor type I;TNF-RI;TNFR-I;p55;p60;CD120a;Tumor necrosis factor receptor superfamily member 1A, membrane form;Tumor necrosis factor-binding protein 1;TBPI;TNFRSF1A;TNFAR, TNFR1; TNFRSF1A CD120a, FPF, TBP1, TNF-R, TNF-R-I, TNF-R55, TNFAR, TNFR1, TNFR55, TNFR60, p55, p55-R, p60 TNF receptor superfamily member 1A tumor necrosis factor receptor superfamily member 1A|TNF-R1|TNF-RI|TNFR-I|tumor necrosis factor binding protein 1|tumor necrosis factor receptor type 1|tumor necrosis factor-alpha receptor
*If product is indicated to react with multiple species, protein info is based on the gene entry specified above in "Species".For more info on TNFRSF1A, check out the TNFRSF1A Infographic
We have 30,000+ of these available, one for each gene! Check them out.
In this infographic, you will see the following information for TNFRSF1A: database IDs, superfamily, protein function, synonyms, molecular weight, chromosomal locations, tissues of expression, subcellular locations, post-translational modifications, and related diseases, research areas & pathways. If you want to see more information included, or would like to contribute to it and be acknowledged, please contact [email protected].
Specific Publications For Anti-TNF Receptor I/TNFRSF1A Antibody Picoband® (PA1210)
Hello CJ!
PA1210 has been cited in 5 publications:
*The publications in this section are manually curated by our staff scientists. They may differ from Bioz's machine gathered results. Both are accurate. If you find a publication citing this product but is missing from this list, please let us know we will issue you a thank-you coupon.
Cytokine expression associated with Helicobacter pylori and Epstein‐Barr virus infection in gastric carcinogenesis
Intravenous Administration Is an Effective and Safe Route for Cancer Gene Therapy Using the Bifidobacterium-Mediated Recombinant HSV-1 Thymidine Kinase and Ganciclovir
Soluble Tumor Necrosis Factor Receptor Mediates Cell Proliferation on Lipopolysaccharide-Stimulated Cultured Human Decidual Stromal Cells
TRAF6 upregulation in spinal astrocytes maintains neuropathic pain by integrating TNF-? and IL-1? signaling
Powerful inner/outer controlled multi-target magnetic nanoparticle drug carrier prepared by liquid photo-immobilization
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Customer Reviews
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Customer Q&As
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5 Customer Q&As for Anti-TNF Receptor I/TNFRSF1A Antibody Picoband®
Question
In the Anti-TNF Receptor I/TNFRSF1A Antibody (PA1210) protocol, the optimal concentration for the primary antibody is 1 μg/ 1 ml and the primary antibody is sold 100 μg/vial, so does that mean that this primary antibody is enough for 100 slides?
Verified customer
Asked: 2022-05-12
Answer
For working concentration of 1ug/ml, 100ug is enough for around 1000 slides. 1ml is enough for around 10 slides.
Boster Scientific Support
Answered: 2022-05-13
Question
Why do I get a band on WB at 75 kD and at 25kD?
Verified Customer
Verified customer
Asked: 2020-01-24
Answer
The additional band at 75kD has been identified as glycosylated TNFR1.
Boster Scientific Support
Answered: 2020-01-24
Question
See below the WB image, lot number and protocol we used for tongue uterus using anti-TNF Receptor I/TNFRSF1A antibody PA1210. Please let me know if you require anything else.
Verified Customer
Verified customer
Asked: 2019-11-21
Answer
Thank you very much for the data. Our lab team are working to resolve this as quickly as possible, and we appreciate your patience and understanding! You have provided everything we needed. Please let me know if there is anything you need in the meantime.
Boster Scientific Support
Answered: 2019-11-21
Question
I was wanting to use your anti-TNF Receptor I/TNFRSF1A antibody for IHC-P for human tongue uterus on frozen tissues, but I want to know if it has been tested for this particular application. Has this antibody been tested and is this antibody a good choice for human tongue uterus identification?
Verified Customer
Verified customer
Asked: 2019-08-29
Answer
It shows on the product datasheet, PA1210 anti-TNF Receptor I/TNFRSF1A antibody has been validated for IHC-P, WB on human tissues. We have an innovator award program that if you test this antibody and show it works in human tongue uterus in IHC-frozen, you can get your next antibody for free.
Boster Scientific Support
Answered: 2019-08-29
Question
We are currently using anti-TNF Receptor I/TNFRSF1A antibody PA1210 for human tissue, and we are well pleased with the WB results. The species of reactivity given in the datasheet says human. Is it possible that the antibody can work on horse tissues as well?
Verified Customer
Verified customer
Asked: 2019-05-21
Answer
The anti-TNF Receptor I/TNFRSF1A antibody (PA1210) has not been validated for cross reactivity specifically with horse tissues, but there is a good chance of cross reactivity. We have an innovator award program that if you test this antibody and show it works in horse you can get your next antibody for free. Please contact me if I can help you with anything.
Boster Scientific Support
Answered: 2019-05-21
