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Product Info Summary
| SKU: | M00002-3 |
|---|---|
| Size: | 100ul |
| Reactive Species: | Human, Mouse, Rat |
| Host: | Mouse |
| Application: | IHC, WB |
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Product info
Product Name
Anti-TNF alpha Monoclonal Antibody
SKU/Catalog Number
M00002-3
Size
100ul
Form
Liquid
Description
Boster Bio Anti-TNF alpha Monoclonal Antibody catalog # M00002-3. Tested in IHC, WB applications. This antibody reacts with Human, Mouse, Rat.
Storage & Handling
Store at -20°C for one year. For short term storage and frequent use, store at 4°C for up to one month. Avoid repeated freeze-thaw cycles.
Cite This Product
Anti-TNF alpha Monoclonal Antibody (Boster Biological Technology, Pleasanton CA, USA, Catalog # M00002-3)
Host
Mouse
Contents
Liquid in PBS containing 50% glycerol, 0.5% BSA and 0.02% sodium azide.
Clonality
Monoclonal
Clone Number
Q34
Isotype
IgG
Immunogen
Synthetic Peptide of TNF α
*Blocking peptide can be purchased. Costs vary based on immunogen length. Contact us for pricing.
Reactive Species
M00002-3 is reactive to TNF in Human, Mouse, Rat
Reconstitution
Calculated molecular weight
25644 MW
Antibody Validation
Boster validates all antibodies on WB, IHC, ICC, Immunofluorescence, and ELISA with known positive control and negative samples to ensure specificity and high affinity, including thorough antibody incubations.
Application & Images
Applications
M00002-3 is guaranteed for IHC, WB Boster Guarantee
Assay Dilutions Recommendation
The recommendations below provide a starting point for assay optimization. The actual working concentration varies and should be decided by the user.
WB 1:1000-1:3000
IHC 1:50-1:200
Validation Images & Assay Conditions
Click image to see more details
Figure 1. Immunohistochemistry validation of TNF using Anti-TNF alpha Monoclonal Antibody (M00002-3).
Immunohistochemistry (IHC) analysis of paraffin-embedded Human Tonsil Tissue using TNF a Mouse Monoclonal Antibody diluted at 1:50.For more protocol information of IHC
Protein Target Info & Infographic
Gene/Protein Information For TNF (Source: Uniprot.org, NCBI)
Gene Name
TNF
Full Name
Tumor necrosis factor
Weight
25644 MW
Superfamily
Tumor necrosis factor family
Alternative Names
Tumor necrosis factor;Cachectin;TNF-alpha;Tumor necrosis factor ligand superfamily member 2;TNF-a;Tumor necrosis factor, membrane form;N-terminal fragment;NTF;Intracellular domain 1;ICD1;Intracellular domain 2;ICD2;C-domain 1;C-domain 2;Tumor necrosis factor, soluble form;TNF;TNFA, TNFSF2; TNF DIF-alpha, TNFA, TNFSF2, TNLG1F, TNF tumor necrosis factor tumor necrosis factor|APC1 protein|TNF, macrophage-derived|TNF, monocyte-derived|TNF-a|tumor necrosis factor ligand 1F|tumor necrosis factor ligand superfamily member 2|tumor necrosis factor-alpha
*If product is indicated to react with multiple species, protein info is based on the gene entry specified above in "Species".For more info on TNF, check out the TNF Infographic
We have 30,000+ of these available, one for each gene! Check them out.
In this infographic, you will see the following information for TNF: database IDs, superfamily, protein function, synonyms, molecular weight, chromosomal locations, tissues of expression, subcellular locations, post-translational modifications, and related diseases, research areas & pathways. If you want to see more information included, or would like to contribute to it and be acknowledged, please contact [email protected].
Specific Publications For Anti-TNF alpha Monoclonal Antibody (M00002-3)
Hello CJ!
M00002-3 has been cited in 7 publications:
*The publications in this section are manually curated by our staff scientists. They may differ from Bioz's machine gathered results. Both are accurate. If you find a publication citing this product but is missing from this list, please let us know we will issue you a thank-you coupon.
Gang Zhong,Huiping Long,Shiting Ma,Yao Shunhan,Jia Li,Jun Yao,miRNA-335-5p relieves chondrocyte inflammation by activating autophagy in osteoarthritis,Life Sciences,Volume 226,2019,Pages 164-172,ISSN 0024-3205,https://doi.org/10.1016/j. lfs.2019.03.071.
Species: Human
M00002-3 usage in article: APP:WB, SAMPLE:CHONDROCYTES, DILUTION:NA
Microstructural changes and immunohistological analysis of pro-inflammatory cytokines in spleens of lipopolysaccharide-induced rats.Indian Journal Of Animal Research.2019.(53):239-244
Species: Rat
M00002-3 usage in article: APP:IHC, SAMPLE:SPLEEN TISSUE, DILUTION:NA
Sun S,Wang Y,Du Y,Sun Q,He L,Zhu E,Li J. Oxidative stress-mediated hepatotoxicity in rats induced by ethanol extracts of different parts of Chloranthus serratus.Pharm Biol.2020 Dec;58(1):1277-1289.doi:10.1080/ n13880209.2020.1859552.PMID: 33355514.
Species: Rat
M00002-3 usage in article: APP:IHC, SAMPLE:LIVER TISSUE, DILUTION:NA
Guo Y,Gu R,Yu J,Lei B,Gan D,Xu G.Synthetic Glucocorticoid-Induced Leucine Zipper Peptide Inhibits Lipopolysaccharide-Induced Ocular Inflammation in Rats.Ophthalmic Res.2020;63(4):434-442.doi:10.1159/000505 003.Epub 2019 Nov 26.PMID:31770752.
Species: Rat
M00002-3 usage in article: APP:WB, SAMPLE:RETINAL TISSUE, DILUTION:1:500
Qi MY,Wang XT,Xu HL,Yang ZL,Cheng Y,Zhou B.Protective effect of ferulic acid on STZ-induced diabetic nephropathy in rats.Food Funct.2020 Apr 1;11(4):3706-3718.doi:10.1039/c9fo02398d.Epub 2020 Apr 20. PMID:32307498.
Species: Rat
M00002-3 usage in article: APP:WB, SAMPLE:KIDNEY TISSUE, DILUTION:1:1000
Zhao B,Li S,Guo Z,Chen Z,Zhang X,Xu C,Chen J,Wei C. Dopamine receptor D2 inhibition alleviates diabetic hepatic stellate cells fibrosis by regulating the TGF-β1/Smads and NFκB pathways. Clin Exp Pharmacol Physiol.2020 Nov 11.doi:10.1111/1440-1681.13437.Ep
Species: Rat
Huang CL,Liu F,Zhang YY,Lin J,Fu M,Li YL, Zhou C,Li CJ,Shen JF. Activation of oxytocin receptor in the trigeminal ganglion attenuates orofacial ectopic pain attributed to inferior alveolar nerve injury. J Neurophysiol.2020 Dec 16.doi:10.1152/jn.00646. 202
Species: Rat
M00002-3 usage in article: APP:WB, SAMPLE:TG CELL AND SPVC CELL, DILUTION:1:1000
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