Click image to see more details
Product Info Summary
| SKU: | PA1352 |
|---|---|
| Size: | 100 μg/vial |
| Reactive Species: | Mouse, Rat |
| Host: | Rabbit |
| Application: | WB |
Customers Who Bought This Also Bought
Product info
Product Name
Anti-Interleukin-6 IL6 Antibody Picoband®
SKU/Catalog Number
PA1352
Size
100 μg/vial
Form
Lyophilized
Description
Boster Bio Anti-Interleukin-6 IL6 Antibody catalog # PA1352. Tested in WB applications. This antibody reacts with Mouse, Rat. The brand Picoband indicates this is a premium antibody that guarantees superior quality, high affinity, and strong signals with minimal background in Western blot applications. Only our best-performing antibodies are designated as Picoband, ensuring unmatched performance.
Storage & Handling
Store at -20˚C for one year from date of receipt. After reconstitution, at 4˚C for one month. It can also be aliquotted and stored frozen at -20˚C for six months. Avoid repeated freeze-thaw cycles.
Cite This Product
Anti-Interleukin-6 IL6 Antibody Picoband® (Boster Biological Technology, Pleasanton CA, USA, Catalog # PA1352)
Host
Rabbit
Contents
Each vial contains 4 mg Trehalose, 0.9 mg NaCl and 0.2 mg Na2HPO4.
Clonality
Polyclonal
Isotype
Rabbit IgG
Immunogen
A synthetic peptide corresponding to a sequence at the C-terminus of rat IL6, different from the related mouse sequence by two amino acids.
*Blocking peptide can be purchased. Costs vary based on immunogen length. Contact us for pricing.
Cross-reactivity
No cross-reactivity with other proteins
Reactive Species
PA1352 is reactive to Il6 in Mouse, Rat
Reconstitution
Add 0.2ml of distilled water will yield a concentration of 500ug/ml.
Observed Molecular Weight
60 kDa
Calculated molecular weight
24357 MW
Background of IL-6
Interleukin-6 (IL-6) is a protein that in humans is encoded by the IL6 gene. IL-6 is an interleukin that acts as both a pro-inflammatory and anti-inflammatory cytokine. It is secreted by T cells and macrophages to stimulate immune response to trauma, especially burns or other tissue damage leading to inflammation. IL-6 is one of the most important mediators of fever and of the acute phase response. IL-6 is also essential for hybridoma growth and is found in many supplemental cloning media such as briclone. Bowcock et al. (1988) assigned the IL6 gene to chromosome 7p21. By in situ hybridization and Southern blot analysis of mouse-human hybrid cell lines, Sutherland et al. (1988) mapped the IL6 gene to chromosome 7p15.
Antibody Validation
Boster validates all antibodies on WB, IHC, ICC, Immunofluorescence, and ELISA with known positive control and negative samples to ensure specificity and high affinity, including thorough antibody incubations.
Application & Images
Applications
PA1352 is guaranteed for WB Boster Guarantee
Assay Dilutions Recommendation
The recommendations below provide a starting point for assay optimization. The actual working concentration varies and should be decided by the user.
Western blot, 0.1-0.5μg/ml, Mouse, Rat
Validation Images & Assay Conditions
Click image to see more details
Anti-IL-6 antibody, PA1352, Western blotting
Lane 1: Recombinant Mouse IL-6 Protein 10ng
Lane 2: Recombinant Mouse IL-6 Protein 5ng
Protein Target Info & Infographic
Gene/Protein Information For Il6 (Source: Uniprot.org, NCBI)
Gene Name
Il6
Full Name
Interleukin-6
Weight
24357 MW
Superfamily
IL-6 superfamily
Alternative Names
Interleukin-6;IL-6;Il6;Il-6; IL6 BSF-2, BSF2, CDF, HGF, HSF, IFN-beta-2, IFNB2, IL-6 interleukin 6 interleukin-6|B-cell differentiation factor|B-cell stimulatory factor 2|CTL differentiation factor|hybridoma growth factor|interferon beta-2|interleukin BSF-2
*If product is indicated to react with multiple species, protein info is based on the gene entry specified above in "Species".For more info on Il6, check out the Il6 Infographic
We have 30,000+ of these available, one for each gene! Check them out.
In this infographic, you will see the following information for Il6: database IDs, superfamily, protein function, synonyms, molecular weight, chromosomal locations, tissues of expression, subcellular locations, post-translational modifications, and related diseases, research areas & pathways. If you want to see more information included, or would like to contribute to it and be acknowledged, please contact [email protected].
Specific Publications For Anti-Interleukin-6 IL6 Antibody Picoband® (PA1352)
Hello CJ!
PA1352 has been cited in 41 publications:
*The publications in this section are manually curated by our staff scientists. They may differ from Bioz's machine gathered results. Both are accurate. If you find a publication citing this product but is missing from this list, please let us know we will issue you a thank-you coupon.
Tan J,Luo J,Meng C,Jiang N,Cao J,Zhao J. Syringin exerts neuroprotective effects in a rat model of cerebral ischemia through the FOXO3a/NF-κB pathway. Int Immunopharmacol.2020 Dec 11;90:107268.doi:10.1016/j. intimp.2020.107268.Epub ahead of print.PMID:333
Species: Rat
PA1352 usage in article: APP:WB, SAMPLE:CEREBRAL CORTEX, DILUTION:NA
Potential Ameliorative Effects of Qing Ye Dan Against Cadmium Induced Prostatic Deficits via Regulating Nrf-2/HO-1 and TGF-%u03B21/Smad Pathways
Transfection with CXCR4 potentiates homing of mesenchymal stem cells in vitro and therapy of diabetic retinopathy in vivo
The expression and significance of IL-6, IFN-%u03B3, SM22%u03B1, and MMP-2 in rat model of aortic dissection
Distribution change of mast cells in human nasal polyps
The Natural Flavone Acacetin Confers Cardiomyocyte Protection Against Hypoxia/Reoxygenation Injury via AMPK-Mediated Activation of Nrf2 Signaling Pathway
Inhibition of endoplasmic reticulum stress signaling pathway: A new mechanism of statins to suppress the development of abdominal aortic aneurysm
Heme Oxygenase-1 Promotes Delayed Wound Healing in Diabetic Rats
Protective effects of thalidomide on pulmonary injuries in a rat model of paraquat intoxication
The effects of HIF-1alpha on gene expression profiles of NCI-H446 human small cell lung cancer cells
Recommended Resources
Here are featured tools and databases that you might find useful.
- Boster's Pathways Library
- Protein Databases
- Bioscience Research Protocol Resources
- Data Processing & Analysis Software
- Photo Editing Software
- Scientific Literature Resources
- Research Paper Management Tools
- Molecular Biology Software
- Primer Design Tools
- Bioinformatics Tools
- Phylogenetic Tree Analysis
Customer Reviews
Have you used Anti-Interleukin-6 IL6 Antibody Picoband®?
Submit a review and receive an Amazon gift card.
- $30 for a review with an image
0 Reviews For Anti-Interleukin-6 IL6 Antibody Picoband®
Customer Q&As
Have a question?
Find answers in Q&As, reviews.
Can't find your answer?
Submit your question
15 Customer Q&As for Anti-Interleukin-6 IL6 Antibody Picoband®
Question
We appreciate helping with my inquiry over the phone. Here are the WB image, lot number and protocol we used for lung using anti-IL6 antibody PA1352. Let me know if you need anything else.
A. Moore
Verified customer
Asked: 2020-04-16
Answer
We appreciate the data. You have provided everything we needed. Our lab team are working to resolve your inquiry as quickly as possible, and we appreciate your patience and understanding! Please let me know if there is anything you need in the meantime.
Boster Scientific Support
Answered: 2020-04-16
Question
Is this PA1352 anti-IL6 antibody reactive to the isotypes of IL6?
Verified Customer
Verified customer
Asked: 2020-03-27
Answer
The immunogen of PA1352 anti-IL6 antibody is A synthetic peptide corresponding to a sequence at the C-terminus of rat IL6(195-211aa KALEEFLKVTMRSTRQT), different from the related mouse sequence by two amino acids. Could you tell me which isotype you are interested in so I can help see if the immunogen is part of this isotype?
Boster Scientific Support
Answered: 2020-03-27
Question
Do you have a BSA free version of anti-IL6 antibody PA1352 available?
Verified Customer
Verified customer
Asked: 2020-03-24
Answer
I appreciate your recent telephone inquiry. I can confirm that some lots of this anti-IL6 antibody PA1352 are BSA free. For now, these lots are available and we can make a BSA free formula for you free of charge. It will take 3 extra days to prepare. If you require this antibody BSA free again in future, please do not hesitate to contact me and I will be pleased to check which lots we have in stock that are BSA free.
Boster Scientific Support
Answered: 2020-03-24
Question
Will anti-IL6 antibody PA1352 work on zebrafish WB with left coronary artery?
Verified Customer
Verified customer
Asked: 2020-02-14
Answer
Our lab technicians have not validated anti-IL6 antibody PA1352 on zebrafish. You can run a BLAST between zebrafish and the immunogen sequence of anti-IL6 antibody PA1352 to see if they may cross-react. If the sequence homology is close, then you can perform a pilot test. Keep in mind that since we have not validated zebrafish samples, this use of the antibody is not covered by our guarantee. However we have an innovator award program that if you test this antibody and show it works in zebrafish left coronary artery in WB, you can get your next antibody for free.
Boster Scientific Support
Answered: 2020-02-14
Question
I was wanting to use your anti-IL6 antibody for WB for mouse lung on frozen tissues, but I want to know if it has been validated for this particular application. Has this antibody been validated and is this antibody a good choice for mouse lung identification?
Verified Customer
Verified customer
Asked: 2020-01-15
Answer
It shows on the product datasheet, PA1352 anti-IL6 antibody has been validated for WB on mouse, rat tissues. We have an innovator award program that if you test this antibody and show it works in mouse lung in IHC-frozen, you can get your next antibody for free.
Boster Scientific Support
Answered: 2020-01-15
Question
Is a blocking peptide available for product anti-IL6 antibody (PA1352)?
Verified Customer
Verified customer
Asked: 2019-09-16
Answer
We do provide the blocking peptide for product anti-IL6 antibody (PA1352). If you would like to place an order for it please contact [email protected] and make a special request.
Boster Scientific Support
Answered: 2019-09-16
Question
We are currently using anti-IL6 antibody PA1352 for rat tissue, and we are satisfied with the WB results. The species of reactivity given in the datasheet says mouse, rat. Is it likely that the antibody can work on goat tissues as well?
L. Gonzalez
Verified customer
Asked: 2019-09-03
Answer
The anti-IL6 antibody (PA1352) has not been tested for cross reactivity specifically with goat tissues, though there is a good chance of cross reactivity. We have an innovator award program that if you test this antibody and show it works in goat you can get your next antibody for free. Please contact me if I can help you with anything.
Boster Scientific Support
Answered: 2019-09-03
Question
We need to test anti-IL6 antibody PA1352 on mouse lung for research purposes, then I may be interested in using anti-IL6 antibody PA1352 for diagnostic purposes as well. Is the antibody suitable for diagnostic purposes?
Verified Customer
Verified customer
Asked: 2019-08-06
Answer
The products we sell, including anti-IL6 antibody PA1352, are only intended for research use. They would not be suitable for use in diagnostic work. If you have the means to develop a product into diagnostic use, and are interested in collaborating with us and develop our product into an IVD product, please contact us for more discussions.
Boster Scientific Support
Answered: 2019-08-06
Question
See below the WB image, lot number and protocol we used for lung using anti-IL6 antibody PA1352. Please let me know if you require anything else.
Verified Customer
Verified customer
Asked: 2019-06-07
Answer
Thank you very much for the data. Our lab team are working to resolve this as quickly as possible, and we appreciate your patience and understanding! You have provided everything we needed. Please let me know if there is anything you need in the meantime.
Boster Scientific Support
Answered: 2019-06-07
Question
I see that the anti-IL6 antibody PA1352 works with WB, what is the protocol used to produce the result images on the product page?
Verified Customer
Verified customer
Asked: 2019-03-08
Answer
You can find protocols for WB on the "support/technical resources" section of our navigation menu. If you have any further questions, please send an email to [email protected]
Boster Scientific Support
Answered: 2019-03-08
Question
Does anti-IL6 antibody PA1352 work for WB with lung?
Verified Customer
Verified customer
Asked: 2018-12-03
Answer
According to the expression profile of lung, IL6 is highly expressed in lung. So, it is likely that anti-IL6 antibody PA1352 will work for WB with lung.
Boster Scientific Support
Answered: 2018-12-03
Question
I have a question about product PA1352, anti-IL6 antibody. I was wondering if it would be possible to conjugate this antibody with biotin. I would need it to be without BSA or sodium azide. I am planning on using a buffer exchange of sodium azide with PBS only. Would there be problems for me to conjugate the antibody and store it in -20 degrees in small aliquots?
Verified Customer
Verified customer
Asked: 2018-10-03
Answer
We do not recommend storing this antibody with PBS buffer only in -20 degrees. If you want to store it in -20 degrees it is best to add some cryoprotectant like glycerol. If you want carrier free PA1352 anti-IL6 antibody, we can provide it to you in a special formula with trehalose and/or glycerol. These molecules will not interfere with conjugation chemistry and provide a good level of protection for the antibody from degradation. Please be sure to specify this in your purchase order.
Boster Scientific Support
Answered: 2018-10-03
Question
We have seen staining in mouse left coronary artery. Are there any suggestions? Is anti-IL6 antibody supposed to stain left coronary artery positively?
G. Mangal
Verified customer
Asked: 2015-07-30
Answer
From literature left coronary artery does express IL6. From Uniprot.org, IL6 is expressed in left coronary artery, fibroblast, lung, among other tissues. Regarding which tissues have IL6 expression, here are a few articles citing expression in various tissues:
Fibroblast, Pubmed ID: 3758081
Lung, Pubmed ID: 15489334
Boster Scientific Support
Answered: 2015-07-30
Question
Our team were satisfied with the WB result of your anti-IL6 antibody. However we have been able to see positive staining in left coronary artery secreted. using this antibody. Is that expected? Could you tell me where is IL6 supposed to be expressed?
L. Jones
Verified customer
Asked: 2014-07-09
Answer
From what I have seen in literature, left coronary artery does express IL6. Generally IL6 expresses in secreted. Regarding which tissues have IL6 expression, here are a few articles citing expression in various tissues:
Fibroblast, Pubmed ID: 3758081
Lung, Pubmed ID: 15489334
Boster Scientific Support
Answered: 2014-07-09
Question
Would PA1352 anti-IL6 antibody work on parafin embedded sections? If so, which fixation method do you recommend we use (PFA, paraformaldehyde, other)?
B. Carter
Verified customer
Asked: 2013-01-31
Answer
It shows on the product datasheet, PA1352 anti-IL6 antibody as been validated on WB. It is best to use PFA for fixation because it has better tissue penetration ability. PFA needs to be prepared fresh before use. Long term stored PFA turns into formalin, as the PFA molecules congregate and become formalin.
Boster Scientific Support
Answered: 2013-01-31
