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Product Info Summary
| SKU: | A31815-1 |
|---|---|
| Size: | 100 µg/vial |
| Reactive Species: | Human, Mouse, Rat |
| Host: | Rabbit |
| Application: | ELISA, Flow Cytometry, WB |
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Product info
Product Name
Anti-TMEM5/RXYLT1 Antibody Picoband®
SKU/Catalog Number
A31815-1
Size
100 µg/vial
Form
Lyophilized
Description
Boster Bio Anti-TMEM5/RXYLT1 Antibody Picoband® catalog # A31815-1. Tested in ELISA, Flow Cytometry, WB applications. This antibody reacts with Human, Mouse, Rat. The brand Picoband indicates this is a premium antibody that guarantees superior quality, high affinity, and strong signals with minimal background in Western blot applications. Only our best-performing antibodies are designated as Picoband, ensuring unmatched performance.
Storage & Handling
At -20°C for one year from date of receipt. After reconstitution, at 4°C for one month. It can also be aliquotted and stored frozen at -20°C for six months. Avoid repeated freezing and thawing.
Cite This Product
Anti-TMEM5/RXYLT1 Antibody Picoband® (Boster Biological Technology, Pleasanton CA, USA, Catalog # A31815-1)
Host
Rabbit
Contents
Each vial contains 4 mg Trehalose, 0.9 mg NaCl, 0.2 mg Na2HPO4.
Clonality
Polyclonal
Isotype
Rabbit IgG
Immunogen
E.coli-derived human TMEM5/RXYLT1 recombinant protein (Position: H79-K398).
*Blocking peptide can be purchased. Costs vary based on immunogen length. Contact us for pricing.
Cross-reactivity
No cross-reactivity with other proteins.
Reactive Species
A31815-1 is reactive to RXYLT1 in Human, Mouse, Rat
Reconstitution
Adding 0.2 ml of distilled water will yield a concentration of 500 µg/ml.
Observed Molecular Weight
56 kDa
Calculated molecular weight
14377 MW
Background of RXYLT1
This gene encodes a type II transmembrane protein that is thought to have glycosyltransferase function. Mutations in this gene result in cobblestone lissencephaly. Alternative splicing results in multiple transcript variants encoding different isoforms.
Antibody Validation
Boster validates all antibodies on WB, IHC, ICC, Immunofluorescence, and ELISA with known positive control and negative samples to ensure specificity and high affinity, including thorough antibody incubations.
Application & Images
Applications
A31815-1 is guaranteed for ELISA, Flow Cytometry, WB Boster Guarantee
Assay Dilutions Recommendation
The recommendations below provide a starting point for assay optimization. The actual working concentration varies and should be decided by the user.
Western blot, 0.25-0.5 μg/ml, Human, Mouse, Rat
Flow Cytometry (Fixed), 1-3 μg/1x106 cells, Human
ELISA, 0.1-0.5 μg/ml, -
Positive Control
WB: human A549 whole cell, human MCF-7 whole cell, human HEL whole cell, rat brain tissue, mouse brain tissue
FCM: Daudi cell
Validation Images & Assay Conditions
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Figure 1. Western blot analysis of TMEM5/RXYLT1 using anti-TMEM5/RXYLT1 antibody (A31815-1).
Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel) / 90V (Resolving gel) for 2-3 hours. The sample well of each lane was loaded with 30 ug of sample under reducing conditions.
Lane 1: human A549 whole cell lysates,
Lane 2: human MCF-7 whole cell lysates,
Lane 3: human HEL whole cell lysates,
Lane 4: rat brain tissue lysates,
Lane 5: mouse brain tissue lysates.
After electrophoresis, proteins were transferred to a nitrocellulose membrane at 150 mA for 50-90 minutes. Blocked the membrane with 5% non-fat milk/TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-TMEM5/RXYLT1 antigen affinity purified polyclonal antibody (Catalog # A31815-1) at 0.5 μg/mL overnight at 4°C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:5000 for 1.5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit (Catalog # EK1002) with Tanon 5200 system. A specific band was detected for TMEM5/RXYLT1 at approximately 56 kDa. The expected band size for TMEM5/RXYLT1 is at 45-51 kDa.
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Figure 2. Flow Cytometry analysis of Daudi cells using anti-TMEM5/RXYLT1 antibody (A31815-1).
Overlay histogram showing Daudi cells stained with A31815-1 (Blue line). To facilitate intracellular staining, cells were fixed with 4% paraformaldehyde and permeabilized with permeabilization buffer. The cells were blocked with 10% normal goat serum. And then incubated with rabbit anti-TMEM5/RXYLT1 Antibody (A31815-1, 1 μg/1x106 cells) for 30 min at 20°C. DyLight®488 conjugated goat anti-rabbit IgG (BA1127, 5-10 μg/1x106 cells) was used as secondary antibody for 30 minutes at 20°C. Isotype control antibody (Green line) was rabbit IgG (1 μg/1x106) used under the same conditions. Unlabelled sample (Red line) was also used as a control.
Protein Target Info & Infographic
Gene/Protein Information For RXYLT1 (Source: Uniprot.org, NCBI)
Gene Name
RXYLT1
Full Name
Ribitol-5-phosphate xylosyltransferase 1
Weight
14377 MW
Superfamily
RXYLT1 family
Alternative Names
Protein Bop; BH3-only protein; Retrotransposon Gag-like protein 10; RTL10; BOP, C22orf29 RXYLT1 HP10481, MDDGA10, TMEM5 ribitol xylosyltransferase 1 ribitol-5-phosphate xylosyltransferase 1|UDP-D-xylose:ribitol-5-phosphate beta1,4-xylosyltransferase|transmembrane protein 5|type II membrane protein
*If product is indicated to react with multiple species, protein info is based on the gene entry specified above in "Species".For more info on RXYLT1, check out the RXYLT1 Infographic
We have 30,000+ of these available, one for each gene! Check them out.
In this infographic, you will see the following information for RXYLT1: database IDs, superfamily, protein function, synonyms, molecular weight, chromosomal locations, tissues of expression, subcellular locations, post-translational modifications, and related diseases, research areas & pathways. If you want to see more information included, or would like to contribute to it and be acknowledged, please contact [email protected].
Specific Publications For Anti-TMEM5/RXYLT1 Antibody Picoband® (A31815-1)
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