Anti-TMEM5/RXYLT1 Antibody Picoband™

Product Name

Anti-TMEM5/RXYLT1 Antibody Picoband™

View all RXYLT1 Antibodies

SKU/Catalog Number

A31815-1

Size

100 µg/vial

Form

Lyophilized

Description

Boster Bio Anti-TMEM5/RXYLT1 Antibody Picoband™ catalog # A31815-1. Tested in ELISA, Flow Cytometry, WB applications. This antibody reacts with Human, Mouse, Rat.

Storage & Handling

At -20°C for one year from date of receipt. After reconstitution, at 4°C for one month. It can also be aliquotted and stored frozen at -20°C for six months. Avoid repeated freezing and thawing.

Cite This Product

Anti-TMEM5/RXYLT1 Antibody Picoband™ (Boster Biological Technology, Pleasanton CA, USA, Catalog # A31815-1)

Host

Rabbit

Contents

Each vial contains 4 mg Trehalose, 0.9 mg NaCl, 0.2 mg Na2HPO4.

Clonality

Polyclonal

Isotype

Rabbit IgG

Immunogen

E.coli-derived human TMEM5/RXYLT1 recombinant protein (Position: H79-K398).

*Blocking peptide can be purchased. Costs vary based on immunogen length. Contact us for pricing.

Cross-reactivity

No cross-reactivity with other proteins.

Reactive Species

A31815-1 is reactive to RXYLT1 in Human, Mouse, Rat

Applications

A31815-1 is guaranteed for ELISA, Flow Cytometry, WB Boster Guarantee

Observed Molecular Weight

56 kDa

Calculated molecular weight

14377 MW

Background of RXYLT1

This gene encodes a type II transmembrane protein that is thought to have glycosyltransferase function. Mutations in this gene result in cobblestone lissencephaly. Alternative splicing results in multiple transcript variants encoding different isoforms.

Antibody Validation

Boster validates all antibodies on WB, IHC, ICC, Immunofluorescence, and ELISA with known positive control and negative samples to ensure specificity and high affinity, including thorough antibody incubations.

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Reconstitution

Adding 0.2 ml of distilled water will yield a concentration of 500 µg/ml.

Assay Dilutions Recommendation

The recommendations below provide a starting point for assay optimization. The actual working concentration varies and should be decided by the user.

Western blot, 0.25-0.5 µg/ml, Human, Mouse, Rat
Flow Cytometry (Fixed), 1-3 µg/1x10⁶ cells, Human
Direct ELISA, 0.1-0.5 µg/ml, Human

Validation Images & Assay Conditions

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Figure 1. Western blot analysis of TMEM5/RXYLT1 using anti-TMEM5/RXYLT1 antibody (A31815-1).
Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel) / 90V (Resolving gel) for 2-3 hours. The sample well of each lane was loaded with 30 ug of sample under reducing conditions.
Lane 1: human A549 whole cell lysates,
Lane 2: human MCF-7 whole cell lysates,
Lane 3: human HEL whole cell lysates,
Lane 4: rat brain tissue lysates,
Lane 5: mouse brain tissue lysates.
After electrophoresis, proteins were transferred to a nitrocellulose membrane at 150 mA for 50-90 minutes. Blocked the membrane with 5% non-fat milk/TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-TMEM5/RXYLT1 antigen affinity purified polyclonal antibody (Catalog # A31815-1) at 0.5 μg/mL overnight at 4°C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:5000 for 1.5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit (Catalog # EK1002) with Tanon 5200 system. A specific band was detected for TMEM5/RXYLT1 at approximately 56 kDa. The expected band size for TMEM5/RXYLT1 is at 45-51 kDa.

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Figure 2. Flow Cytometry analysis of Daudi cells using anti-TMEM5/RXYLT1 antibody (A31815-1).
Overlay histogram showing Daudi cells stained with A31815-1 (Blue line). To facilitate intracellular staining, cells were fixed with 4% paraformaldehyde and permeabilized with permeabilization buffer. The cells were blocked with 10% normal goat serum. And then incubated with rabbit anti-TMEM5/RXYLT1 Antibody (A31815-1, 1 μg/1x10⁶ cells) for 30 min at 20°C. DyLight®488 conjugated goat anti-rabbit IgG (BA1127, 5-10 μg/1x10⁶ cells) was used as secondary antibody for 30 minutes at 20°C. Isotype control antibody (Green line) was rabbit IgG (1 μg/1x10⁶) used under the same conditions. Unlabelled sample (Red line) was also used as a control.

Gene/Protein Information For RXYLT1 (Source: Uniprot.org, NCBI)

Alternative Names

Ribitol-5-phosphate xylosyltransferase 1 RXYLT1 HP10481, MDDGA10, TMEM5 ribitol xylosyltransferase 1 ribitol-5-phosphate xylosyltransferase 1|UDP-D-xylose:ribitol-5-phosphate beta1,4-xylosyltransferase|transmembrane protein 5|type II membrane protein

For more info on RXYLT1, check out the RXYLT1 Infographic

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In this infographic, you will see the following information for RXYLT1: database IDs, superfamily, protein function, synonyms, molecular weight, chromosomal locations, tissues of expression, subcellular locations, post-translational modifications, and related diseases, research areas & pathways. If you want to see more information included, or would like to contribute to it and be acknowledged, please contact [email protected].

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