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Product Info Summary
| SKU: | MA1112 |
|---|---|
| Size: | 100 μg/vial |
| Reactive Species: | Human, Mouse, Rat |
| Host: | Mouse |
| Application: | IF, IHC, ICC, WB |
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Product info
Product Name
Anti-Beta-Tubulin TUBB1 Antibody (Monoclonal, TUB 2.1)
SKU/Catalog Number
MA1112
Size
100 μg/vial
Form
Lyophilized
Description
Boster Bio Anti-Beta-Tubulin TUBB1 Antibody (Monoclonal, TUB 2.1) catalog # MA1112. Tested in IF, IHC, ICC, WB applications. This antibody reacts with Human, Mouse, Rat.
Storage & Handling
Store at -20˚C for one year from date of receipt. After reconstitution, at 4˚C for one month. It can also be aliquotted and stored frozen at -20˚C for six months. Avoid repeated freeze-thaw cycles.
Cite This Product
Anti-Beta-Tubulin TUBB1 Antibody (Monoclonal, TUB 2.1) (Boster Biological Technology, Pleasanton CA, USA, Catalog # MA1112)
Host
Mouse
Contents
Mouse ascites fluid, 1.2% sodium acetate, 2mg BSA, with 0.01mg NaN3 as preservative.
Clonality
Monoclonal
Clone Number
TUB 2.1
Isotype
Mouse IgG1
Immunogen
Tubulin from rat brain.
*Blocking peptide can be purchased. Costs vary based on immunogen length. Contact us for pricing.
Cross-reactivity
No cross-reactivity with other proteins
Reactive Species
MA1112 is reactive to TUBB1 in Human, Mouse, Rat
Reconstitution
Add 1ml of PBS buffer will yield a concentration of 100ug/ml.
Observed Molecular Weight
55 kDa
Calculated molecular weight
50327 MW
Background of TUBB1
Tubulin beta (TUBB) was derived from its corresponding mRNA with a molecular weight of about 55,000. TUBB gene contains 4 exons and located in the HLA class I region at 6p21.3. Paclitaxel resistance in non-small-cell lung cancer associated with beta-tubulin gene mutations.
Antibody Validation
Boster validates all antibodies on WB, IHC, ICC, Immunofluorescence, and ELISA with known positive control and negative samples to ensure specificity and high affinity, including thorough antibody incubations.
Application & Images
Applications
MA1112 is guaranteed for IF, IHC, ICC, WB Boster Guarantee
Assay Dilutions Recommendation
The recommendations below provide a starting point for assay optimization. The actual working concentration varies and should be decided by the user.
Immunohistochemistry (Paraffin-embedded Section), 0.5-1μg/ml, Human, mouse, rat, By Heat
Western blot, 2μg/ml, Human, mouse, rat
Immunocytochemistry/Immunofluorescence,5μg/ml, Human
Positive Control
WB: Rat Skeletal Muscle Tissue
IHC: Human Mammary Cancer Tissue
Validation Images & Assay Conditions
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Anti-beta-Tubulin antibody (monoclonal), MA1112, Western blotting
WB: Rat Skeletal Muscle Tissue Lysate
Click image to see more details
Anti-beta-Tubulin antibody (monoclonal), MA1112, IHC(P)
IHC(P): Human Mammary Cancer Tissue
Protein Target Info & Infographic
Gene/Protein Information For TUBB1 (Source: Uniprot.org, NCBI)
Gene Name
TUBB1
Full Name
Tubulin beta-1 chain
Weight
50327 MW
Superfamily
tubulin family
Alternative Names
Tubulin beta-1 chain;TUBB1; TUBB1 tubulin beta 1 class VI tubulin beta-1 chain|tubulin, beta 1
*If product is indicated to react with multiple species, protein info is based on the gene entry specified above in "Species".For more info on TUBB1, check out the TUBB1 Infographic
We have 30,000+ of these available, one for each gene! Check them out.
In this infographic, you will see the following information for TUBB1: database IDs, superfamily, protein function, synonyms, molecular weight, chromosomal locations, tissues of expression, subcellular locations, post-translational modifications, and related diseases, research areas & pathways. If you want to see more information included, or would like to contribute to it and be acknowledged, please contact [email protected].
Specific Publications For Anti-Beta-Tubulin TUBB1 Antibody (Monoclonal, TUB 2.1) (MA1112)
Hello CJ!
MA1112 has been cited in 19 publications:
*The publications in this section are manually curated by our staff scientists. They may differ from Bioz's machine gathered results. Both are accurate. If you find a publication citing this product but is missing from this list, please let us know we will issue you a thank-you coupon.
Label-retaining assay enriches tumor-initiating cells in glioblastoma spheres cultivated in serum-free medium
Han Zhang,Huaishan Wang,Fei Gao,Jia Yang,Yi Xu,Yi Fu,Menghua Cai,Xue Zhang,Qi Yang,Kexin Tong,Yu Hu,Hui Chen,Chao Ma,Wei He,Jianmin Zhang,TSPO Deficiency Accelerates Amyloid Pathology and Neuroinflammation by Impairing Microglial Phagocytosis,Neurobiology of Aging,2021,,ISSN 0197-4580,https://doi.org/10.1016/j.neurobiolaging.2021.06.020.
Species: Human,Mouse
MA1112 usage in article: APP:WB, SAMPLE:BRAIN TISSUE, DILUTION:NA
Shi,W.,Que,Y.,Zhang,X.et al.Functional tissue-engineered bone-like graft made of a fibrin scaffold and TG2 gene-modified EMSCs for bone defect repair.NPG Asia Mater 13,28 (2021).https://doi.org/10.1038/s41427-021-00297-w
Species: Rat
MA1112 usage in article: APP:WB, SAMPLE:EMSCS, DILUTION:NA
Fan J,He M,Wang CJ,Zhang M. Gadolinium Chloride Inhibits the Production of Liver Interleukin-27 and Mitigates Liver Injury in the CLP Mouse Model. Mediators Inflamm. 2021 Jan 29;2021:2605973. doi:10.1155/2021/2605973.PMID:33564275;PMCID:PMC7867451.
Species: Mouse
Fan J,He M,Wang CJ,Zhang M. Gadolinium Chloride Inhibits the Production of Liver Interleukin-27 and Mitigates Liver Injury in the CLP Mouse Model. Mediators Inflamm. 2021 Jan 29;2021:2605973.doi:10.1155/ 2021/2605973.PMID:33564275;PMCID:PMC7867451.
Species: Mouse
Xu T,Song Q,Zhou L,Yang W,Wu X,Qian Q,Chai H,Han Q,Pan H,Dou X,Li S.Ferulic acid alleviates lipotoxicity-induced hepatocellular death through the SIRT1-regulated autophagy pathway and independently of AMPK and Akt in AML-12 hepatocytes.Nutr Metab (Lond).2
Species: Mouse
MA1112 usage in article: APP:WB, SAMPLE:AML-12 CELL, DILUTION:NA
Xu T,Song Q,Zhou L,Yang W,Wu X,Qian Q,Chai H,Han Q,Pan H,Dou X,Li S. Ferulic acid alleviates lipotoxicity-induced hepatocellular death through the SIRT1-regulated autophagy pathway and independently of AMPK and Akt in AML-12 hepatocytes. Nutr Metab (Lond)
Species: Mouse
MA1112 usage in article: APP:WB, SAMPLE:AML-12 CELLS, DILUTION:NA
Guan Y,Chen X,Zhao B,Shi Y,Han F.What Happened in the Hippocampal Axon in a Rat Model of Posttraumatic Stress Disorder.Cell Mol Neurobiol.2020 Sep 15.doi:10.1007/s10571-020-00960-w.Epub ahead of print.PMID:32930942.
Species: Rat
MA1112 usage in article: APP:IF, SAMPLE:BRAIN TISSUE, DILUTION:1:200
Fan J,Zhang YC,Zheng DF,Zhang M,Liu H,He M,Wu ZJ.IL-27 is elevated in sepsis with acute hepatic injury and promotes hepatic damage and inflammation in the CLP model. Cytokine.2020 Mar;127:154936.doi: 10.1016/j.cyto.2019.154936.Epub 2019 Nov 28.PMID:317865
Species: Human, Mouse
MA1112 usage in article: APP:WB, SAMPLE:LIVER, DILUTION:1:1000
Propofol induces apoptosis and inhibits the proliferation of rat embryonic neural stem cells via gamma-aminobutyric acid type A receptor
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Customer Reviews
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Customer Q&As
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4 Customer Q&As for Anti-Beta-Tubulin TUBB1 Antibody (Monoclonal, TUB 2.1)
Question
Will anti-Beta-Tubulin antibody (Monoclonal, TUB 2.1) MA1112 work on dog IHC with brain?
Verified Customer
Verified customer
Asked: 2019-08-27
Answer
Our lab technicians have not tested anti-Beta-Tubulin antibody (Monoclonal, TUB 2.1) MA1112 on dog. You can run a BLAST between dog and the immunogen sequence of anti-Beta-Tubulin antibody (Monoclonal, TUB 2.1) MA1112 to see if they may cross-react. If the sequence homology is close, then you can perform a pilot test. Keep in mind that since we have not validated dog samples, this use of the antibody is not covered by our guarantee. However we have an innovator award program that if you test this antibody and show it works in dog brain in IHC, you can get your next antibody for free.
Boster Scientific Support
Answered: 2019-08-27
Question
We are currently using anti-Beta-Tubulin antibody (Monoclonal, TUB 2.1) MA1112 for rat tissue, and we are well pleased with the IHC results. The species of reactivity given in the datasheet says human, mouse, rat. Is it possible that the antibody can work on pig tissues as well?
E. Walker
Verified customer
Asked: 2018-10-17
Answer
The anti-Beta-Tubulin antibody (Monoclonal, TUB 2.1) (MA1112) has not been tested for cross reactivity specifically with pig tissues, but there is a good chance of cross reactivity. We have an innovator award program that if you test this antibody and show it works in pig you can get your next antibody for free. Please contact me if I can help you with anything.
Boster Scientific Support
Answered: 2018-10-17
Question
I appreciate helping with my inquiry over the phone. Here are the WB image, lot number and protocol we used for platelet using anti-Beta-Tubulin antibody (Monoclonal, TUB 2.1) MA1112. Let me know if you need anything else.
P. Mangal
Verified customer
Asked: 2018-08-06
Answer
We appreciate the data. You have provided everything we needed. Our lab team are working to resolve your inquiry as quickly as possible, and we appreciate your patience and understanding! Please let me know if there is anything you need in the meantime.
Boster Scientific Support
Answered: 2018-08-06
Question
We are interested in to test anti-Beta-Tubulin antibody (Monoclonal, TUB 2.1) MA1112 on human platelet for research purposes, then I may be interested in using anti-Beta-Tubulin antibody (Monoclonal, TUB 2.1) MA1112 for diagnostic purposes as well. Is the antibody suitable for diagnostic purposes?
S. Anderson
Verified customer
Asked: 2017-05-12
Answer
The products we sell, including anti-Beta-Tubulin antibody (Monoclonal, TUB 2.1) MA1112, are only intended for research use. They would not be suitable for use in diagnostic work. If you have the means to develop a product into diagnostic use, and are interested in collaborating with us and develop our product into an IVD product, please contact us for more discussions.
Boster Scientific Support
Answered: 2017-05-12
