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Product Info Summary
| SKU: | M05893 |
|---|---|
| Size: | 100 μl/vial |
| Reactive Species: | Human, Mouse, Rat |
| Host: | Rabbit |
| Application: | IHC, WB |
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Product info
Product Name
Anti-TMEM43 Rabbit Monoclonal Antibody
SKU/Catalog Number
M05893
Size
100 μl/vial
Form
Liquid
Description
Boster Bio Anti-TMEM43 Rabbit Monoclonal Antibody catalog # M05893. Tested in WB, IHC applications. This antibody reacts with Human, Mouse, Rat.
Storage & Handling
Store at -20°C for one year. For short term storage and frequent use, store at 4°C for up to one month. Avoid repeated freeze-thaw cycles.
Cite This Product
Anti-TMEM43 Rabbit Monoclonal Antibody (Boster Biological Technology, Pleasanton CA, USA, Catalog # M05893)
Host
Rabbit
Contents
Rabbit IgG in phosphate buffered saline, pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol, 0.4-0.5mg/ml BSA.
Clonality
Monoclonal
Clone Number
30T30
Isotype
IgG
Immunogen
A synthesized peptide derived from human TMEM43
*Blocking peptide can be purchased. Costs vary based on immunogen length. Contact us for pricing.
Reactive Species
M05893 is reactive to TMEM43 in Human, Mouse, Rat
Reconstitution
Restore with deionized water (or equivalent) for reconstitution volume of 1.0 mL
Observed Molecular Weight
45 kDa
Calculated molecular weight
113086 MW
Antibody Validation
Boster validates all antibodies on WB, IHC, ICC, Immunofluorescence, and ELISA with known positive control and negative samples to ensure specificity and high affinity, including thorough antibody incubations.
Application & Images
Applications
M05893 is guaranteed for IHC, WB Boster Guarantee
Assay Dilutions Recommendation
The recommendations below provide a starting point for assay optimization. The actual working concentration varies and should be decided by the user.
WB 1:500-1:2000
IHC 1:50-1:200
Positive Control
WB: human Jurkat whole cell, human Hacat whole cell, human SiHa whole cell, human PC-3 whole cell, rat C6 whole cell, mouse Neuro-2a whole cell
Validation Images & Assay Conditions
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Figure 1. Western blot analysis of TMEM43 using anti-TMEM43 antibody (M05893).
Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel) / 90V (Resolving gel) for 2-3 hours. The sample well of each lane was loaded with 30 ug of sample under reducing conditions.
Lane 1: human Jurkat whole cell lysates,
Lane 2: human Hacat whole cell lysates,
Lane 3: human SiHa whole cell lysates,
Lane 4: human PC-3 whole cell lysates,
Lane 5: rat C6 whole cell lysates,
Lane 6: mouse Neuro-2a whole cell lysates.
After electrophoresis, proteins were transferred to a nitrocellulose membrane at 150 mA for 50-90 minutes. Blocked the membrane with 5% non-fat milk/TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-TMEM43 antigen affinity purified monoclonal antibody (Catalog # M05893) at 1:500 overnight at 4°C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:1000 for 1.5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit (Catalog # EK1002) with Tanon 5200 system. A specific band was detected for TMEM43 at approximately 45 kDa. The expected band size for TMEM43 is at 45 kDa.
Protein Target Info & Infographic
Gene/Protein Information For TMEM43 (Source: Uniprot.org, NCBI)
Gene Name
TMEM43
Full Name
Transmembrane protein 43
Weight
113086 MW
Superfamily
TMEM43 family
Alternative Names
Golgin subfamily A member 2;130 kDa cis-Golgi matrix protein ;GM130 ;GM130 autoantigen ;Golgin-95 ;GOLGA2; Tmem43|1200015A22Rik, LU, LUMA|transmembrane protein 43|transmembrane protein 43
*If product is indicated to react with multiple species, protein info is based on the gene entry specified above in "Species".For more info on TMEM43, check out the TMEM43 Infographic
We have 30,000+ of these available, one for each gene! Check them out.
In this infographic, you will see the following information for TMEM43: database IDs, superfamily, protein function, synonyms, molecular weight, chromosomal locations, tissues of expression, subcellular locations, post-translational modifications, and related diseases, research areas & pathways. If you want to see more information included, or would like to contribute to it and be acknowledged, please contact [email protected].
Specific Publications For Anti-TMEM43 Rabbit Monoclonal Antibody (M05893)
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