Product Info Summary
SKU: | A11580-1 |
---|---|
Size: | 100 μg/vial |
Reactive Species: | Human, Mouse, Rat |
Host: | Rabbit |
Application: | Flow Cytometry, IHC, IHC-F, ICC, WB |
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Product info
Product Name
Anti-TMEM166/EVA1A Antibody Picoband®
SKU/Catalog Number
A11580-1
Size
100 μg/vial
Form
Lyophilized
Description
Boster Bio Anti-TMEM166/EVA1A Antibody Picoband® catalog # A11580-1. Tested in Flow Cytometry, IHC, IHC-F, ICC, WB applications. This antibody reacts with Human, Mouse, Rat. The brand Picoband indicates this is a premium antibody that guarantees superior quality, high affinity, and strong signals with minimal background in Western blot applications. Only our best-performing antibodies are designated as Picoband, ensuring unmatched performance.
Storage & Handling
Store at -20˚C for one year from date of receipt. After reconstitution, at 4˚C for one month. It can also be aliquotted and stored frozen at -20˚C for six months. Avoid repeated freeze-thaw cycles.
Cite This Product
Anti-TMEM166/EVA1A Antibody Picoband® (Boster Biological Technology, Pleasanton CA, USA, Catalog # A11580-1)
Host
Rabbit
Contents
Each vial contains 4mg Trehalose, 0.9mg NaCl, 0.2mg Na2HPO4, 0.05mg NaN3.
Clonality
Polyclonal
Isotype
Rabbit IgG
Immunogen
A synthetic peptide corresponding to a sequence at the N-terminus of human TMEM166/EVA1A, which shares 66.7% and 50% amino acid (aa) sequence identity with mouse and rat TMEM166/EVA1A, respectively.
*Blocking peptide can be purchased. Costs vary based on immunogen length. Contact us for pricing.
Cross-reactivity
No cross-reactivity with other proteins.
Reactive Species
A11580-1 is reactive to EVA1A in Human, Mouse, Rat
Reconstitution
Observed Molecular Weight
21 kDa
Calculated molecular weight
17.47kDa
Background of TMEM166
Eva-1 homolog A (C. elegans) is a protein that in humans is encoded by the EVA1A gene. It belongs to the FAM176 family. This gene is mapped to 2p12. EVA1A, also called TMEM166, acts as a regulator of programmed cell death, mediating both autophagy and apoptosis.
Antibody Validation
Boster validates all antibodies on WB, IHC, ICC, Immunofluorescence, and ELISA with known positive control and negative samples to ensure specificity and high affinity, including thorough antibody incubations.
Application & Images
Applications
A11580-1 is guaranteed for Flow Cytometry, IHC, IHC-F, ICC, WB Boster Guarantee
Positive Control
WB: human HepG2 whole cell, human PC-3 whole cell
IHC: human liver cancer tissue, human renal cancer tissue
FCM: A549 cell
Validation Images & Assay Conditions
Click image to see more details
Figure 1. Western blot analysis of EVA1A using anti-EVA1A antibody (A11580-1).
Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel) / 90V (Resolving gel) for 2-3 hours. The sample well of each lane was loaded with 50ug of sample under reducing conditions.
Lane 1: human HepG2 whole cell lysates,
Lane 2: human PC-3 whole cell lysates.
After Electrophoresis, proteins were transferred to a Nitrocellulose membrane at 150mA for 50-90 minutes. Blocked the membrane with 5% Non-fat Milk/ TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-EVA1A antigen affinity purified polyclonal antibody (Catalog # A11580-1) at 0.5 μg/mL overnight at 4°C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:10000 for 1.5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit (Catalog # EK1002) with Tanon 5200 system. A specific band was detected for EVA1A at approximately 21KD. The expected band size for EVA1A is at 17KD.
Click image to see more details
Figure 2. IHC analysis of EVA1A using anti-EVA1A antibody (A11580-1).
EVA1A was detected in paraffin-embedded section of human liver cancer tissue. Heat mediated antigen retrieval was performed in citrate buffer (pH6, epitope retrieval solution) for 20 mins. The tissue section was blocked with 10% goat serum. The tissue section was then incubated with 1μg/ml rabbit anti-EVA1A Antibody (A11580-1) overnight at 4°C. Biotinylated goat anti-rabbit IgG was used as secondary antibody and incubated for 30 minutes at 37°C. The tissue section was developed using Strepavidin-Biotin-Complex (SABC)(Catalog # SA1022) with DAB as the chromogen.
Click image to see more details
Figure 3. IHC analysis of EVA1A using anti-EVA1A antibody (A11580-1).
EVA1A was detected in paraffin-embedded section of human renal cancer tissue. Heat mediated antigen retrieval was performed in citrate buffer (pH6, epitope retrieval solution) for 20 mins. The tissue section was blocked with 10% goat serum. The tissue section was then incubated with 1μg/ml rabbit anti-EVA1A Antibody (A11580-1) overnight at 4°C. Biotinylated goat anti-rabbit IgG was used as secondary antibody and incubated for 30 minutes at 37°C. The tissue section was developed using Strepavidin-Biotin-Complex (SABC)(Catalog # SA1022) with DAB as the chromogen.
Click image to see more details
Figure 4. Flow Cytometry analysis of A549 cells using anti-EVA1A antibody (A11580-1).
Overlay histogram showing A549 cells stained with A11580-1 (Blue line). To facilitate intracellular staining, cells were fixed with 4% paraformaldehyde and permeabilized with permeabilization buffer. The cells were blocked with 10% normal goat serum. And then incubated with rabbit anti-EVA1A Antibody (A11580-1,1μg/1x106 cells) for 30 min at 20°C. DyLight®488 conjugated goat anti-rabbit IgG (BA1127, 5-10μg/1x106 cells) was used as secondary antibody for 30 minutes at 20°C. Isotype control antibody (Green line) was rabbit IgG (1μg/1x106) used under the same conditions. Unlabelled sample (Red line) was also used as a control.
Protein Target Info & Infographic
Gene/Protein Information For EVA1A (Source: Uniprot.org, NCBI)
Gene Name
EVA1A
Full Name
Protein eva-1 homolog A
Weight
17.47kDa
Superfamily
EVA1 family
Alternative Names
Protein eva-1 homolog A; Protein FAM176A; Transmembrane protein 166; EVA1A; FAM176A; TMEM166; SP24 EVA1A FAM176A, TMEM166 eva-1 homolog A, regulator of programmed cell death protein eva-1 homolog A|family with sequence similarity 176, member A|protein FAM176A|transmembrane protein 166
*If product is indicated to react with multiple species, protein info is based on the gene entry specified above in "Species".For more info on EVA1A, check out the EVA1A Infographic
We have 30,000+ of these available, one for each gene! Check them out.
In this infographic, you will see the following information for EVA1A: database IDs, superfamily, protein function, synonyms, molecular weight, chromosomal locations, tissues of expression, subcellular locations, post-translational modifications, and related diseases, research areas & pathways. If you want to see more information included, or would like to contribute to it and be acknowledged, please contact [email protected].
Specific Publications For Anti-TMEM166/EVA1A Antibody Picoband® (A11580-1)
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Customer Q&As
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3 Customer Q&As for Anti-TMEM166/EVA1A Antibody Picoband®
Question
We are currently using anti-TMEM166/EVA1A antibody A11580-1 for rat tissue, and we are happy with the ICC results. The species of reactivity given in the datasheet says human, mouse, rat. Is it possible that the antibody can work on bovine tissues as well?
Verified Customer
Verified customer
Asked: 2020-03-19
Answer
The anti-TMEM166/EVA1A antibody (A11580-1) has not been validated for cross reactivity specifically with bovine tissues, though there is a good chance of cross reactivity. We have an innovator award program that if you test this antibody and show it works in bovine you can get your next antibody for free. Please contact me if I can help you with anything.
Boster Scientific Support
Answered: 2020-03-19
Question
Is there a BSA free version of anti-TMEM166/EVA1A antibody A11580-1 available?
Verified Customer
Verified customer
Asked: 2019-09-20
Answer
We appreciate your recent telephone inquiry. I can confirm that some lots of this anti-TMEM166/EVA1A antibody A11580-1 are BSA free. For now, these lots are available and we can make a BSA free formula for you free of charge. It will take 3 extra days to prepare. If you require this antibody BSA free again in future, please do not hesitate to contact me and I will be pleased to check which lots we have in stock that are BSA free.
Boster Scientific Support
Answered: 2019-09-20
Question
Does anti-TMEM166/EVA1A antibody A11580-1 work on pig ICC with placenta?
J. Johnson
Verified customer
Asked: 2019-02-13
Answer
Our lab technicians have not tested anti-TMEM166/EVA1A antibody A11580-1 on pig. You can run a BLAST between pig and the immunogen sequence of anti-TMEM166/EVA1A antibody A11580-1 to see if they may cross-react. If the sequence homology is close, then you can perform a pilot test. Keep in mind that since we have not validated pig samples, this use of the antibody is not covered by our guarantee. However we have an innovator award program that if you test this antibody and show it works in pig placenta in ICC, you can get your next antibody for free.
Boster Scientific Support
Answered: 2019-02-13