Anti-TMEM166/EVA1A Antibody Picoband®

TMEM166 antibody

Boster Bio Anti-TMEM166/EVA1A Antibody Picoband® catalog # A11580-1. Tested in Flow Cytometry, IHC, IHC-F, ICC, WB applications. This antibody reacts with Human, Mouse, Rat. The brand Picoband indicates this is a premium antibody that guarantees superior quality, high affinity, and strong signals with minimal background in Western blot applications. Only our best-performing antibodies are designated as Picoband, ensuring unmatched performance.

Product Info Summary

SKU: A11580-1
Size: 100 μg/vial
Reactive Species: Human, Mouse, Rat
Host: Rabbit
Application: Flow Cytometry, IHC, IHC-F, ICC, WB

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Product Name

Anti-TMEM166/EVA1A Antibody Picoband®

View all TMEM166 Antibodies

SKU/Catalog Number

A11580-1

Size

100 μg/vial

Form

Lyophilized

Description

Boster Bio Anti-TMEM166/EVA1A Antibody Picoband® catalog # A11580-1. Tested in Flow Cytometry, IHC, IHC-F, ICC, WB applications. This antibody reacts with Human, Mouse, Rat. The brand Picoband indicates this is a premium antibody that guarantees superior quality, high affinity, and strong signals with minimal background in Western blot applications. Only our best-performing antibodies are designated as Picoband, ensuring unmatched performance.

Storage & Handling

Store at -20˚C for one year from date of receipt. After reconstitution, at 4˚C for one month. It can also be aliquotted and stored frozen at -20˚C for six months. Avoid repeated freeze-thaw cycles.

Cite This Product

Anti-TMEM166/EVA1A Antibody Picoband® (Boster Biological Technology, Pleasanton CA, USA, Catalog # A11580-1)

Host

Rabbit

Contents

Each vial contains 4mg Trehalose, 0.9mg NaCl, 0.2mg Na2HPO4, 0.05mg NaN3.

Clonality

Polyclonal

Isotype

Rabbit IgG

Immunogen

A synthetic peptide corresponding to a sequence at the N-terminus of human TMEM166/EVA1A, which shares 66.7% and 50% amino acid (aa) sequence identity with mouse and rat TMEM166/EVA1A, respectively.

*Blocking peptide can be purchased. Costs vary based on immunogen length. Contact us for pricing.

Cross-reactivity

No cross-reactivity with other proteins.

Reactive Species

A11580-1 is reactive to EVA1A in Human, Mouse, Rat

Reconstitution

Observed Molecular Weight

21 kDa

Calculated molecular weight

17.47kDa

Background of TMEM166

Eva-1 homolog A (C. elegans) is a protein that in humans is encoded by the EVA1A gene. It belongs to the FAM176 family. This gene is mapped to 2p12. EVA1A, also called TMEM166, acts as a regulator of programmed cell death, mediating both autophagy and apoptosis.

Antibody Validation

Boster validates all antibodies on WB, IHC, ICC, Immunofluorescence, and ELISA with known positive control and negative samples to ensure specificity and high affinity, including thorough antibody incubations.

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Applications

A11580-1 is guaranteed for Flow Cytometry, IHC, IHC-F, ICC, WB Boster Guarantee

Positive Control

WB: human HepG2 whole cell, human PC-3 whole cell
IHC: human liver cancer tissue, human renal cancer tissue
FCM: A549 cell

Validation Images & Assay Conditions

Gene/Protein Information For EVA1A (Source: Uniprot.org, NCBI)

Gene Name

EVA1A

Full Name

Protein eva-1 homolog A

Weight

17.47kDa

Superfamily

EVA1 family

Alternative Names

Protein eva-1 homolog A; Protein FAM176A; Transmembrane protein 166; EVA1A; FAM176A; TMEM166; SP24 EVA1A FAM176A, TMEM166 eva-1 homolog A, regulator of programmed cell death protein eva-1 homolog A|family with sequence similarity 176, member A|protein FAM176A|transmembrane protein 166

*If product is indicated to react with multiple species, protein info is based on the gene entry specified above in "Species".

For more info on EVA1A, check out the EVA1A Infographic

EVA1A infographic

We have 30,000+ of these available, one for each gene! Check them out.

In this infographic, you will see the following information for EVA1A: database IDs, superfamily, protein function, synonyms, molecular weight, chromosomal locations, tissues of expression, subcellular locations, post-translational modifications, and related diseases, research areas & pathways. If you want to see more information included, or would like to contribute to it and be acknowledged, please contact [email protected].

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3 Customer Q&As for Anti-TMEM166/EVA1A Antibody Picoband®

Question

We are currently using anti-TMEM166/EVA1A antibody A11580-1 for rat tissue, and we are happy with the ICC results. The species of reactivity given in the datasheet says human, mouse, rat. Is it possible that the antibody can work on bovine tissues as well?

Verified Customer

Verified customer

Asked: 2020-03-19

Answer

The anti-TMEM166/EVA1A antibody (A11580-1) has not been validated for cross reactivity specifically with bovine tissues, though there is a good chance of cross reactivity. We have an innovator award program that if you test this antibody and show it works in bovine you can get your next antibody for free. Please contact me if I can help you with anything.

Boster Scientific Support

Answered: 2020-03-19

Question

Is there a BSA free version of anti-TMEM166/EVA1A antibody A11580-1 available?

Verified Customer

Verified customer

Asked: 2019-09-20

Answer

We appreciate your recent telephone inquiry. I can confirm that some lots of this anti-TMEM166/EVA1A antibody A11580-1 are BSA free. For now, these lots are available and we can make a BSA free formula for you free of charge. It will take 3 extra days to prepare. If you require this antibody BSA free again in future, please do not hesitate to contact me and I will be pleased to check which lots we have in stock that are BSA free.

Boster Scientific Support

Answered: 2019-09-20

Question

Does anti-TMEM166/EVA1A antibody A11580-1 work on pig ICC with placenta?

J. Johnson

Verified customer

Asked: 2019-02-13

Answer

Our lab technicians have not tested anti-TMEM166/EVA1A antibody A11580-1 on pig. You can run a BLAST between pig and the immunogen sequence of anti-TMEM166/EVA1A antibody A11580-1 to see if they may cross-react. If the sequence homology is close, then you can perform a pilot test. Keep in mind that since we have not validated pig samples, this use of the antibody is not covered by our guarantee. However we have an innovator award program that if you test this antibody and show it works in pig placenta in ICC, you can get your next antibody for free.

Boster Scientific Support

Answered: 2019-02-13

Order DetailsPrice
A11580-1

100μg

$370
A11580-1-10ug

10μg sample (liquid)

$99
A11580-1-Biotin

100 μg Biotin conjugated

$570
A11580-1-Cy3

100 μg Cy3 conjugated

$570
A11580-1-Dylight488

100 μg Dylight488 conjugated

$570
A11580-1-Dylight550

100 μg Dylight550 conjugated

$570
A11580-1-Dylight594

100 μg Dylight594 conjugated

$570
A11580-1-FITC

100 μg FITC conjugated

$570
A11580-1-HRP

100 μg HRP conjugated

$570
A11580-1-APC

100 μg APC conjugated

$670
A11580-1-PE

100 μg PE conjugated

$670
A11580-1-iFluor647

100 μg iFluor647 conjugated

$670
A11580-1-carrier-free

Carrier Free

$370

More conjugates/formats

Free Secondary Antibody

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A11580-1
Buy one primary antibody get one 0.5ml HRP or Biotin secondary antibody for free.
*Sample sizes are prepared on demand and will take extra lead time. (cannot be conjugated)
$370.00

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