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Product Info Summary
| SKU: | A00827-1 |
|---|---|
| Size: | 0.1 mg |
| Reactive Species: | Human, Mouse, Rat |
| Host: | Rabbit |
| Application: | ELISA, IF, IHC-P, WB |
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Product info
Product Name
Anti-GTP-binding protein Rheb Rheb Antibody
SKU/Catalog Number
A00827-1
Size
0.1 mg
Form
Liquid
Description
Boster Bio Anti-GTP-binding protein Rheb Rheb Antibody (Catalog # A00827-1). Tested in ELISA, WB, IHC-P, IF applications. This antibody reacts with Human, Mouse, Rat.
Storage & Handling
Rheb antibody can be stored at 4°C for three months and -20°C, stable for up to one year. Avoid repeated freeze-thaw cycles. Antibodies should not be exposed to prolonged high temperatures.
Cite This Product
Anti-GTP-binding protein Rheb Rheb Antibody (Boster Biological Technology, Pleasanton CA, USA, Catalog # A00827-1)
Host
Rabbit
Contents
Rheb Antibody is supplied in PBS containing 0.02% sodium azide.
Clonality
Polyclonal
Isotype
IgG
Immunogen
Rheb antibody was raised against a 15 amino acid synthetic peptide from the middle region of human Rheb. The immunogen is located within amino acids 40 - 90 of Rheb.
*Blocking peptide can be purchased. Costs vary based on immunogen length. Contact us for pricing.
Cross-reactivity
Post-translationally modified Rheb is sometimes observed at higher molecular weight.
Reactive Species
A00827-1 is reactive to RHEB in Human, Mouse, Rat
Reconstitution
Observed Molecular Weight
68 kDa
Calculated molecular weight
20497 MW
Background of RHEB
Rheb (Ras homolog enriched in brain) is an evolutionarily conserved member of the Ras family of small GTP-binding proteins originally found to be rapidly induced by synaptic activity in the hippocampus following seizure. While it is expressed at relatively high levels in the brain, Rheb is widely expressed in other tissues and may be induced by growth factor stimulation. Similar to other family members, Rheb triggers activation of the Raf-MEK-MAPK pathway. Biochemical and genetic studies demonstrate that Rheb has an important role in regulating the insulin/Target of rapamycin (TOR) signaling pathway. TOR is a serine/threonine protein kinase that acts as a sensor for ATP and amino acids, balancing the availability of nutrients with protein translation and cell growth. A dimeric protein complex termed TSC1/TSC2 indirectly inhibits TOR activity by inhibiting Rheb via the GAP activity of TSC2.
Antibody Validation
Boster validates all antibodies on WB, IHC, ICC, Immunofluorescence, and ELISA with known positive control and negative samples to ensure specificity and high affinity, including thorough antibody incubations.
Application & Images
Applications
A00827-1 is guaranteed for ELISA, IF, IHC-P, WB Boster Guarantee
Assay Dilutions Recommendation
The recommendations below provide a starting point for assay optimization. The actual working concentration varies and should be decided by the user.
Rheb antibody can be used for the detection of Rheb by Western blot at 1 to 4 μg/mL. Antibody can also be used for immunohistochemistry starting at 2 μg/mL. For immunofluorescence start at 20 μg/mL.
Antibody validated: Western Blot in mouse samples; Immunohistochemistry in mouse samples and Immunofluorescence in mouse samples. All other applications and species not yet tested. Optimal dilutions for each application should be determined by the researcher.
Validation Images & Assay Conditions
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Western blot analysis of Rheb in mouse brain cell lysate with Rheb antibody at (A) 1
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Immunohistochemistry of Rheb in mouse brain tissue with Rheb antibody at 2 μg/mL.
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Immunofluorescence of Rheb in Mouse Brain cells with Rheb antibody at 20 μg/mL.
Protein Target Info & Infographic
Gene/Protein Information For RHEB (Source: Uniprot.org, NCBI)
Gene Name
RHEB
Full Name
GTP-binding protein Rheb
Weight
20497 MW
Superfamily
small GTPase superfamily
Alternative Names
RHEB2, RHEB2, GTP-binding protein Rheb, Ras homolog enriched in brain RHEB RHEB2 Ras homolog, mTORC1 binding GTP-binding protein Rheb|Ras homolog enriched in brain 2
*If product is indicated to react with multiple species, protein info is based on the gene entry specified above in "Species".For more info on RHEB, check out the RHEB Infographic
We have 30,000+ of these available, one for each gene! Check them out.
In this infographic, you will see the following information for RHEB: database IDs, superfamily, protein function, synonyms, molecular weight, chromosomal locations, tissues of expression, subcellular locations, post-translational modifications, and related diseases, research areas & pathways. If you want to see more information included, or would like to contribute to it and be acknowledged, please contact [email protected].
Specific Publications For Anti-GTP-binding protein Rheb Rheb Antibody (A00827-1)
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