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Product Info Summary
| SKU: | A12868-1 |
|---|---|
| Size: | 100 μg/vial |
| Reactive Species: | Human |
| Host: | Rabbit |
| Application: | ELISA, WB |
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Product info
Product Name
Anti-TM9SF3 Antibody Picoband®
SKU/Catalog Number
A12868-1
Size
100 μg/vial
Form
Lyophilized
Description
Boster Bio Anti-TM9SF3 Antibody Picoband® catalog # A12868-1. Tested in ELISA, WB applications. This antibody reacts with Human. The brand Picoband indicates this is a premium antibody that guarantees superior quality, high affinity, and strong signals with minimal background in Western blot applications. Only our best-performing antibodies are designated as Picoband, ensuring unmatched performance.
Storage & Handling
At -20°C for one year from date of receipt. After reconstitution, at 4°C for one month. It can also be aliquotted and stored frozen at -20°C for six months. Avoid repeated freezing and thawing.
Cite This Product
Anti-TM9SF3 Antibody Picoband® (Boster Biological Technology, Pleasanton CA, USA, Catalog # A12868-1)
Host
Rabbit
Contents
Each vial contains 4 mg Trehalose, 0.9 mg NaCl, 0.2 mg Na2HPO4.
Clonality
Polyclonal
Isotype
Rabbit IgG
Immunogen
E.coli-derived human TM9SF3 recombinant protein (Position: H31-M465).
*Blocking peptide can be purchased. Costs vary based on immunogen length. Contact us for pricing.
Cross-reactivity
No cross-reactivity with other proteins.
Reactive Species
A12868-1 is reactive to TM9SF3 in Human
Reconstitution
Adding 0.2 ml of distilled water will yield a concentration of 500 µg/ml.
Observed Molecular Weight
72 kDa
Calculated molecular weight
67.888kDa
Background of TM9SF3
Predicted to be involved in protein localization to membrane. Predicted to be located in exocytic vesicle. Predicted to be active in membrane.
Antibody Validation
Boster validates all antibodies on WB, IHC, ICC, Immunofluorescence, and ELISA with known positive control and negative samples to ensure specificity and high affinity, including thorough antibody incubations.
Application & Images
Applications
A12868-1 is guaranteed for ELISA, WB Boster Guarantee
Assay Dilutions Recommendation
The recommendations below provide a starting point for assay optimization. The actual working concentration varies and should be decided by the user.
Western blot, 0.25-0.5 μg/ml, Human
ELISA, 0.1-0.5 μg/ml, -
Positive Control
WB: human U251 whole cell, human A431 whole cell
Validation Images & Assay Conditions
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Figure 1. Western blot analysis of TM9SF3 using anti-TM9SF3 antibody (A12868-1).
Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel) / 90V (Resolving gel) for 2-3 hours. The sample well of each lane was loaded with 30 ug of sample under reducing conditions.
Lane 1: human U251 whole cell lysates,
Lane 2: human A431 whole cell lysates.
After electrophoresis, proteins were transferred to a nitrocellulose membrane at 150 mA for 50-90 minutes. Blocked the membrane with 5% non-fat milk/TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-TM9SF3 antigen affinity purified polyclonal antibody (Catalog # A12868-1) at 0.5 μg/mL overnight at 4°C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:5000 for 1.5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit (Catalog # EK1002) with Tanon 5200 system. A specific band was detected for TM9SF3 at approximately 72 kDa. The expected band size for TM9SF3 is at 68 kDa.
Protein Target Info & Infographic
Gene/Protein Information For TM9SF3 (Source: Uniprot.org, NCBI)
Gene Name
TM9SF3
Full Name
Transmembrane 9 superfamily member 3
Weight
67.888kDa
Superfamily
nonaspanin (TM9SF) (TC 9.A.2) family
Alternative Names
Mediator of RNA polymerase II transcription subunit 9; Mediator complex subunit 9; MED9; MED25; TM9SF3 EP70-P-iso, SMBP transmembrane 9 superfamily member 3 transmembrane 9 superfamily member 3|SM-11044-binding protein|dinucleotide oxidase disulfide thiol exchanger 3 superfamily member 3|endomembrane protein emp70 precursor isolog
*If product is indicated to react with multiple species, protein info is based on the gene entry specified above in "Species".For more info on TM9SF3, check out the TM9SF3 Infographic
We have 30,000+ of these available, one for each gene! Check them out.
In this infographic, you will see the following information for TM9SF3: database IDs, superfamily, protein function, synonyms, molecular weight, chromosomal locations, tissues of expression, subcellular locations, post-translational modifications, and related diseases, research areas & pathways. If you want to see more information included, or would like to contribute to it and be acknowledged, please contact [email protected].
Specific Publications For Anti-TM9SF3 Antibody Picoband® (A12868-1)
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