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Product Info Summary
| SKU: | A08611 |
|---|---|
| Size: | 0.1 mg |
| Reactive Species: | Human, Mouse |
| Host: | Rabbit |
| Application: | ELISA, IHC-P, WB |
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Product info
Product Name
Anti-MED28 Antibody
SKU/Catalog Number
A08611
Size
0.1 mg
Form
Liquid
Description
Boster Bio Anti-MED28 Antibody (Catalog # A08611). Tested in ELISA, WB, IHC-P applications. This antibody reacts with Human, Mouse.
Storage & Handling
MED28 antibody can be stored at 4°C for three months and -20°C, stable for up to one year. Avoid repeated freeze-thaw cycles. Antibodies should not be exposed to prolonged high temperatures.
Cite This Product
Anti-MED28 Antibody (Boster Biological Technology, Pleasanton CA, USA, Catalog # A08611)
Host
Rabbit
Contents
MED28 Antibody is supplied in PBS containing 0.02% sodium azide.
Clonality
Polyclonal
Isotype
IgG
Immunogen
MED28 antibody was raised against a 17 amino acid synthetic peptide from near the center of human MED28. The immunogen is located within amino acids 50 - 100 of MED28.
*Blocking peptide can be purchased. Costs vary based on immunogen length. Contact us for pricing.
Cross-reactivity
DCNP1 antibody is human, mouse and rat reactive.
Reactive Species
A08611 is reactive to MED28 in Human, Mouse
Reconstitution
Observed Molecular Weight
68 kDa
Calculated molecular weight
19520 MW
Background of Med28
The mediator complex is a multi-protein transcriptional co-activator that is expressed ubiquitously in eukaryotes from yeast to mammals and is required for induction of RNA polymerase II (pol II) transcription by DNA binding transcription factor. One of the proteins in this complex is MED28, also known as Magicin. MED28 is expressed in many cell lines and tissues. It has been shown that a down-regulation of MED28 expression in NIH3T3 cells results in a significant induction of several genes associated with smooth muscle cell differentiation and conversely its over-expression represses expression of SMC genes. MED28 can also form a ternary complex with Grb2 and Merlin, the neurofibromatosis 2 tumor suppressor protein, indicating that MED28 may play a role in Merlin's tumor suppressive activity. MED28 has also been recently identified as an HIV dependency factor (HDF), suggesting that MED28 may be an important drug target in HIV treatment. At least two isoforms of MED28 are known to exist.
Antibody Validation
Boster validates all antibodies on WB, IHC, ICC, Immunofluorescence, and ELISA with known positive control and negative samples to ensure specificity and high affinity, including thorough antibody incubations.
Application & Images
Applications
A08611 is guaranteed for ELISA, IHC-P, WB Boster Guarantee
Assay Dilutions Recommendation
The recommendations below provide a starting point for assay optimization. The actual working concentration varies and should be decided by the user.
MED28 antibody can be used for detection of MED28 by Western blot at 1 μg/mL. Antibody can also be used for immunohistochemistry starting at 2.5 μg/mL. For immunofluorescence start at 20 μg/mL.
Antibody validated: Western Blot in human samples and Immunohistochemistry in human samples. All other applications and species not yet tested. Optimal dilutions for each application should be determined by the researcher.
Validation Images & Assay Conditions
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Western blot analysis of MED28 in human brain tissue lysate with MED28 antibody at 1 μg/mL in (A) the absence and (B) the presence of blocking peptide.
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Immunohistochemistry of MED28 in human brain tissue with MED28 antibody at 2.5 μg/mL.
Protein Target Info & Infographic
Gene/Protein Information For MED28 (Source: Uniprot.org, NCBI)
Gene Name
MED28
Full Name
Mediator of RNA polymerase II transcription subunit 28
Weight
19520 MW
Superfamily
Mediator complex subunit 28 family
Alternative Names
EG1, magicin, 1500003D12Rik, EG1, FKSG20, Mediator of RNA polymerase II transcription subunit 28, Endothelial-derived protein 1, Magicin Med28|1500003D12Rik, AI451633, AU045690, Eg1, FKSG20, mag, magicin|mediator complex subunit 28|mediator of RNA polymerase II transcription subunit 28|endothelial-derived|mediator of RNA polymerase II transcription, subunit 28 homolog
*If product is indicated to react with multiple species, protein info is based on the gene entry specified above in "Species".For more info on MED28, check out the MED28 Infographic
We have 30,000+ of these available, one for each gene! Check them out.
In this infographic, you will see the following information for MED28: database IDs, superfamily, protein function, synonyms, molecular weight, chromosomal locations, tissues of expression, subcellular locations, post-translational modifications, and related diseases, research areas & pathways. If you want to see more information included, or would like to contribute to it and be acknowledged, please contact [email protected].
Specific Publications For Anti-MED28 Antibody (A08611)
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