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Product Info Summary
| SKU: | A01393-3 |
|---|---|
| Size: | 100 μg/vial |
| Reactive Species: | Human |
| Host: | Rabbit |
| Application: | ELISA, Flow Cytometry, WB |
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Product info
Product Name
Anti-Tip60/KAT5 Antibody Picoband®
SKU/Catalog Number
A01393-3
Size
100 μg/vial
Form
Lyophilized
Description
Boster Bio Anti-Tip60/KAT5 Antibody Picoband® catalog # A01393-3. Tested in ELISA, Flow Cytometry, WB applications. This antibody reacts with Human. The brand Picoband indicates this is a premium antibody that guarantees superior quality, high affinity, and strong signals with minimal background in Western blot applications. Only our best-performing antibodies are designated as Picoband, ensuring unmatched performance.
Storage & Handling
At -20°C for one year from date of receipt. After reconstitution, at 4°C for one month. It can also be aliquotted and stored frozen at -20°C for six months. Avoid repeated freezing and thawing.
Cite This Product
Anti-Tip60/KAT5 Antibody Picoband® (Boster Biological Technology, Pleasanton CA, USA, Catalog # A01393-3)
Host
Rabbit
Contents
Each vial contains 4 mg Trehalose, 0.9 mg NaCl, 0.2 mg Na2HPO4.
Clonality
Polyclonal
Isotype
Rabbit IgG
Immunogen
E.coli-derived human Tip60/KAT5 recombinant protein (Position: M1-E56).
*Blocking peptide can be purchased. Costs vary based on immunogen length. Contact us for pricing.
Cross-reactivity
No cross-reactivity with other proteins.
Reactive Species
A01393-3 is reactive to KAT5 in Human
Reconstitution
Adding 0.2 ml of distilled water will yield a concentration of 500 μg/ml.
Observed Molecular Weight
60 kDa
Calculated molecular weight
9670 MW
Background of TIP60
Histone acetyltransferase KAT5 is an enzyme that in humans is encoded by the KAT5 gene. The protein encoded by this gene belongs to the MYST family of histone acetyl transferases (HATs) and was originally isolated as an HIV-1 TAT-interactive protein. HATs play important roles in regulating chromatin remodeling, transcription and other nuclear processes by acetylating histone and nonhistone proteins. This protein is a histone acetylase that has a role in DNA repair and apoptosis and is thought to play an important role in signal transduction. Alternative splicing of this gene results in multiple transcript variants.
Antibody Validation
Boster validates all antibodies on WB, IHC, ICC, Immunofluorescence, and ELISA with known positive control and negative samples to ensure specificity and high affinity, including thorough antibody incubations.
Application & Images
Applications
A01393-3 is guaranteed for ELISA, Flow Cytometry, WB Boster Guarantee
Assay Dilutions Recommendation
The recommendations below provide a starting point for assay optimization. The actual working concentration varies and should be decided by the user.
Western blot, 0.25-0.5 μg/ml, Human
Flow Cytometry (Fixed), 1-3 μg/1x106 cells, Human
ELISA, 0.1-0.5 μg/ml, -
Positive Control
WB: human Jurkat whole cell, human CCRF-CEM whole cell, human 293T whole cell, human Hela whole cell, human MCF-7 whole cell
FCM: THP-1 cell
Validation Images & Assay Conditions
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Figure 1. Western blot analysis of Tip60/KAT5 using anti-Tip60/KAT5 antibody (A01393-3).
Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel) / 90V (Resolving gel) for 2-3 hours. The sample well of each lane was loaded with 30 ug of sample under reducing conditions.
Lane 1: human Jurkat whole cell lysates,
Lane 2: human CCRF-CEM whole cell lysates,
Lane 3: human 293T whole cell lysates,
Lane 4: human Hela whole cell lysates,
Lane 5: human MCF-7 whole cell lysates.
After electrophoresis, proteins were transferred to a nitrocellulose membrane at 150 mA for 50-90 minutes. Blocked the membrane with 5% non-fat milk/TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-Tip60/KAT5 antigen affinity purified polyclonal antibody (Catalog # A01393-3) at 0.5 μg/mL overnight at 4°C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:5000 for 1.5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit (Catalog # EK1002) with Tanon 5200 system. A specific band was detected for Tip60/KAT5 at approximately 60 kDa. The expected band size for Tip60/KAT5 is at 59 kDa.
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Figure 2. Flow Cytometry analysis of THP-1 cells using anti-Tip60/KAT5 antibody (A01393-3).
Overlay histogram showing THP-1 cells stained with A01393-3 (Blue line). To facilitate intracellular staining, cells were fixed with 4% paraformaldehyde and permeabilized with permeabilization buffer. The cells were blocked with 10% normal goat serum. And then incubated with rabbit anti-Tip60/KAT5 Antibody (A01393-3, 1 μg/1x106 cells) for 30 min at 20°C. DyLight®488 conjugated goat anti-rabbit IgG (BA1127, 5-10 μg/1x106 cells) was used as secondary antibody for 30 minutes at 20°C. Isotype control antibody (Green line) was rabbit IgG (1 μg/1x106) used under the same conditions. Unlabelled sample without incubation with primary antibody and secondary antibody (Red line) was used as a blank control.
Protein Target Info & Infographic
Gene/Protein Information For KAT5 (Source: Uniprot.org, NCBI)
Gene Name
KAT5
Full Name
Histone acetyltransferase KAT5
Weight
9670 MW
Superfamily
MYST (SAS/MOZ) family
Alternative Names
Retinal dehydrogenase 1; RALDH 1; RalDH1; ALDH-E1; ALHDII; Aldehyde dehydrogenase family 1 member A; Aldehyde dehydrogenase, cytosolic; ALDH1A1; ALDC; ALDH1 KAT5 ESA1, HTATIP, HTATIP1, NEDFASB, PLIP, TIP, TIP60, ZC2HC5, cPLA2 lysine acetyltransferase 5 histone acetyltransferase KAT5|60 kDa Tat-interactive protein|HIV-1 Tat interactive protein, 60kDa|K(lysine) acetyltransferase 5|K-acetyltransferase 5|Tat interacting protein, 60kDa|cPLA(2)-interacting protein|cPLA2 interacting protein|histone acetyltransferase HTATIP
*If product is indicated to react with multiple species, protein info is based on the gene entry specified above in "Species".For more info on KAT5, check out the KAT5 Infographic
We have 30,000+ of these available, one for each gene! Check them out.
In this infographic, you will see the following information for KAT5: database IDs, superfamily, protein function, synonyms, molecular weight, chromosomal locations, tissues of expression, subcellular locations, post-translational modifications, and related diseases, research areas & pathways. If you want to see more information included, or would like to contribute to it and be acknowledged, please contact [email protected].
Specific Publications For Anti-Tip60/KAT5 Antibody Picoband® (A01393-3)
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