Anti-TIMP-3 (Tissue Inhibitor of Metalloproteinase-3) Antibody

Product Name

Anti-TIMP-3 (Tissue Inhibitor of Metalloproteinase-3) Antibody

View all TIMP-3 Antibodies

SKU/Catalog Number

M00477

Size

100ug/vial

Form

Liquid

Description

Boster Bio Anti-TIMP-3 (Tissue Inhibitor of Metalloproteinase-3) Antibody (Catalog # M00477). Tested in Flow Cytometry, IF, IHC applications. This antibody reacts with Human, Mouse, Rat, Horse, Cow, Dog.

Storage & Handling

Antibody with azide - store at 2 to 8°C. Antibody without azide - store at -20 to -80°C. Antibody is stable for 24 months. Non-hazardous. No MSDS required.

Cite This Product

Anti-TIMP-3 (Tissue Inhibitor of Metalloproteinase-3) Antibody (Boster Biological Technology, Pleasanton CA, USA, Catalog # M00477)

Host

Rabbit

Contents

Prepared in 10mM PBS with 0.05% BSA & 0.05% azide. Also available WITHOUT BSA at 1.0mg/ml.

Clonality

Polyclonal

Isotype

IgG

Immunogen

The amino acid sequence (aa175-211), used as the immunogen for TIMP3 Ab, is 100% homologous in human, cow, dog and horse, and 94% homologous in Mouse and rat.

*Blocking peptide can be purchased. Costs vary based on immunogen length. Contact us for pricing.

Reactive Species

M00477 is reactive to TIMP3 in Cow, Dog, Human, Mouse, Rat, Horse

Reconstitution

Reconstitute with 1ml water (15 minutes at room temperature).

Calculated molecular weight

24145 MW

Background of TIMP-3

TIMP3 (tissue inhibitor of metalloproteinases 3), along with family members TIMP1, TIMP2, and TIMP4, are inhibitors of the matrix metalloproteinases (MMPs), a group of peptidases involved in degradation of the extracellular matrix (ECM). An imbalance between MMPs and the associated TIMPs may play a significant role in the invasive phenotype of malignant tumors. TIMP's inhibit the proteolytic invasiveness of tumor cells and normal placental trophoblast cells. TIMP-3 may be involved in regulating trophoblastic invasion of the uterus as well as in regulating remodeling of the extracellular matrix during the folding of epithelia, and in the formation, branching and expansion of epithelial tubes.

Antibody Validation

Boster validates all antibodies on WB, IHC, ICC, Immunofluorescence, and ELISA with known positive control and negative samples to ensure specificity and high affinity, including thorough antibody incubations.

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Applications

M00477 is guaranteed for Flow Cytometry, IF, IHC Boster Guarantee

Assay Dilutions Recommendation

The recommendations below provide a starting point for assay optimization. The actual working concentration varies and should be decided by the user.

Flow Cytometry (1-2ug/million cells)
Immunofluorescence (1-2ug/ml)
Immunohistochemistry (Formalin-fixed) (1-2ug/ml for 30 minutes at RT)(Staining of formalin-fixed tissues requires heating tissue sections in 10mM Tris with 1mM EDTA, pH 9.0, for 45 min at 95°C followed by cooling at RT for 20 minutes)
Optimal dilution for a specific application should be determined.

Validation Images & Assay Conditions

Gene/Protein Information For TIMP3 (Source: Uniprot.org, NCBI)

Alternative Names

Metalloproteinase inhibitor 3;Protein MIG-5;Tissue inhibitor of metalloproteinases 3;TIMP-3;TIMP3; TIMP3 HSMRK222, K222, K222TA2, SFD TIMP metallopeptidase inhibitor 3 metalloproteinase inhibitor 3|MIG-5 protein|TIMP-3|protein MIG-5|tissue inhibitor of metalloproteinases 3

*If product is indicated to react with multiple species, protein info is based on the gene entry specified above in "Species".

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