Anti-TIM-4 TIMD4 Antibody

Product Name

Anti-TIM-4 TIMD4 Antibody

View all TIM-4 Antibodies

SKU/Catalog Number

A06637-1

Size

0.1 mg

Form

Liquid

Description

Boster Bio Anti-TIM-4 TIMD4 Antibody (Catalog # A06637-1). Tested in ELISA, WB, ICC, IF, IHC-P applications. This antibody reacts with Human, Mouse.

Storage & Handling

TIM-4 antibody can be stored at 4°C for three months and -20°C, stable for up to one year. Avoid repeated freeze-thaw cycles. Antibodies should not be exposed to prolonged high temperatures.

Cite This Product

Anti-TIM-4 TIMD4 Antibody (Boster Biological Technology, Pleasanton CA, USA, Catalog # A06637-1)

Host

Rabbit

Contents

TIM-4 Antibody is supplied in PBS containing 0.02% sodium azide.

Clonality

Polyclonal

Isotype

IgG

Immunogen

TIM-4 antibody was raised against a 16 amino acid synthetic peptide from near the center of human TIM-4. The immunogen is located within amino acids 60 - 110 of TIM-4.

*Blocking peptide can be purchased. Costs vary based on immunogen length. Contact us for pricing.

Cross-reactivity

At least four isoforms of TIM-4 are known to exist; this antibody will detect all four isoforms.

Reactive Species

A06637-1 is reactive to TIMD4 in Human, Mouse

Reconstitution

Observed Molecular Weight

68 kDa

Calculated molecular weight

41578 MW

Background of TIM-4

The T cell immunoglobulin and mucin domain containing protein (TIM) family encodes cell surface receptors that are involved in the regulation of T helper (Th) -1 and -2 cell-mediated immunity. TIM-4, which is preferentially expressed on macrophages and dendritic cells, is the natural ligand of TIM-1, and this binding leads to T-cell expansion and cytokine production. Unlike other members of the TIM family, TIM-4 lacks a putative tyrosine phosphorylation signal sequence in its intracellular domain. The TIM-4 gene maps to a locus associated with predisposition to asthma in both mice and humans and with its connection to TIM-1-triggered Th2 responsiveness, may be considered as a candidate disease/predisposition gene for asthma.

Antibody Validation

Boster validates all antibodies on WB, IHC, ICC, Immunofluorescence, and ELISA with known positive control and negative samples to ensure specificity and high affinity, including thorough antibody incubations.

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Applications

A06637-1 is guaranteed for ELISA, IF, IHC-P, ICC, WB Boster Guarantee

Assay Dilutions Recommendation

The recommendations below provide a starting point for assay optimization. The actual working concentration varies and should be decided by the user.

TIM-4 antibody can be used for the detection of TIM-4 by Western blot at 2 μg/mL. Antibody can also be used for immunocytochemistry starting at 10 μg/mL and Immunohistochemistry starting at 2 μg/mL. For immunofluorescence start at 20 μg/mL.
Antibody validated: Western Blot in mouse samples; Immunohistochemistry in human and mouse samples; Immunocytochemistry in human samples and Immunofluorescence in human samples. All other applications and species not yet tested. Optimal dilutions for each application should be determined by the researcher.

Validation Images & Assay Conditions

Gene/Protein Information For TIMD4 (Source: Uniprot.org, NCBI)

Alternative Names

TIM4, SMUCKLER, TIM4, T-cell immunoglobulin and mucin domain-containing protein 4, T-cell immunoglobulin mucin receptor 4, TIMD-4 TIMD4 SMUCKLER, TIM4 T cell immunoglobulin and mucin domain containing 4 T-cell immunoglobulin and mucin domain-containing protein 4|T-cell immunoglobulin mucin receptor 4|T-cell membrane protein 4|TIM-4|TIMD-4

*If product is indicated to react with multiple species, protein info is based on the gene entry specified above in "Species".

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