Click image to see more details
Product Info Summary
| SKU: | A00657 |
|---|---|
| Size: | 0.1 mg |
| Reactive Species: | Human |
| Host: | Rabbit |
| Application: | ELISA, IF, IHC-P, WB |
Customers Who Bought This Also Bought
Product info
Product Name
Anti-TIM-3 HAVCR2 Antibody
SKU/Catalog Number
A00657
Size
0.1 mg
Form
Liquid
Description
Boster Bio Anti-TIM-3 HAVCR2 Antibody (Catalog # A00657). Tested in ELISA, WB, IHC-P, IF applications. This antibody reacts with Human.
Storage & Handling
TIM-3 antibody can be stored at 4°C for three months and -20°C, stable for up to one year. Avoid repeated freeze-thaw cycles. Antibodies should not be exposed to prolonged high temperatures.
Cite This Product
Anti-TIM-3 HAVCR2 Antibody (Boster Biological Technology, Pleasanton CA, USA, Catalog # A00657)
Host
Rabbit
Contents
TIM-3 Antibody is supplied in PBS containing 0.02% sodium azide.
Clonality
Polyclonal
Isotype
IgG
Immunogen
TIM-3 antibody was raised against a peptide corresponding to 16 amino acids near the amino terminus of human TIM-3. The immunogen is located within amino acids 60 - 110 of TIM-3.
*Blocking peptide can be purchased. Costs vary based on immunogen length. Contact us for pricing.
Cross-reactivity
At least two antibodies are known to exist; this antibody will detect both isoforms.
Reactive Species
A00657 is reactive to HAVCR2 in Human
Reconstitution
Observed Molecular Weight
68 kDa
Calculated molecular weight
33394 MW
Background of TIM-3
The immune checkpoint protein TIM-3 is a member of the immunoglobulin superfamily and TIM family of proteins that was initially identified as a specific marker of fully differentiated IFN-γ producing CD4 T helper 1 (Th1) and CD8 cytotoxic cells. It is a Th1-specific cell surface protein that regulates macrophage activation and negatively regulates Th1-mediated auto- and alloimmune responses, and is also highly expressed on regulatory T cells, monocytes, macrophages, and dendritic cells (1). TIM-3 and PD-1 are co-expressed on most CD4 and CD8 T cells infiltrating solid tumors or in hematologic malignancy in mice; blocking TIM-3 in conjugation with a PD-1 blockade increases the functionality of exhausted T cells and synergizes with to inhibit tumor growth (2,3).
Antibody Validation
Boster validates all antibodies on WB, IHC, ICC, Immunofluorescence, and ELISA with known positive control and negative samples to ensure specificity and high affinity, including thorough antibody incubations.
Application & Images
Applications
A00657 is guaranteed for ELISA, IF, IHC-P, WB Boster Guarantee
Assay Dilutions Recommendation
The recommendations below provide a starting point for assay optimization. The actual working concentration varies and should be decided by the user.
TIM-3 antibody can be used for Western blot at 1 - 2 μg/mL. Antibody can also be used for Immunohistochemistry at 2 μg/mL. For Immunoflorescence start at 20 μg/mL.
Antibody validated: Western Blot in human samples; Immunohistochemistry in human samples and Immunofluorescence in human samples. All other applications and species not yet tested. Optimal dilutions for each application should be determined by the researcher.
Validation Images & Assay Conditions
Click image to see more details
Western blot analysis of TIM-3 in Jurkat cell lysate with TIM-3 antibody at 1 μg/mL in (A) the absence and (B) the presence of blocking peptide.
Click image to see more details
Immunohistochemistry of TIM-3 in human spleen tissue with TIM-3 antibody at 2 μg/ml.
Click image to see more details
Immunofluorescence of TIM-3 in human spleen tissue with TIM-3 antibody at 20 μg/ml.
Red: TIM-3 Antibody (A00657)
Blue: DAPI staining
Protein Target Info & Infographic
Gene/Protein Information For HAVCR2 (Source: Uniprot.org, NCBI)
Gene Name
HAVCR2
Full Name
Hepatitis A virus cellular receptor 2
Weight
33394 MW
Superfamily
immunoglobulin superfamily
Alternative Names
Hepatitis A virus cellular receptor, HAVCR2, TIM-3, CD366, KIM-3, TIMD3, TIMD-3 HAVCR2 CD366, HAVcr-2, KIM-3, SPTCL, TIM3, TIMD-3, TIMD3, Tim-3 hepatitis A virus cellular receptor 2 hepatitis A virus cellular receptor 2|T cell immunoglobulin mucin 3|T-cell immunoglobulin and mucin domain-containing protein 3|T-cell immunoglobulin mucin family member 3|T-cell immunoglobulin mucin receptor 3|T-cell membrane protein 3|kidney injury molecule-3
*If product is indicated to react with multiple species, protein info is based on the gene entry specified above in "Species".For more info on HAVCR2, check out the HAVCR2 Infographic
We have 30,000+ of these available, one for each gene! Check them out.
In this infographic, you will see the following information for HAVCR2: database IDs, superfamily, protein function, synonyms, molecular weight, chromosomal locations, tissues of expression, subcellular locations, post-translational modifications, and related diseases, research areas & pathways. If you want to see more information included, or would like to contribute to it and be acknowledged, please contact [email protected].
Specific Publications For Anti-TIM-3 HAVCR2 Antibody (A00657)
Hello CJ!
No publications found for A00657
*Do you have publications using this product? Share with us and receive a reward. Ask us for more details.
Recommended Resources
Here are featured tools and databases that you might find useful.
- Boster's Pathways Library
- Protein Databases
- Bioscience Research Protocol Resources
- Data Processing & Analysis Software
- Photo Editing Software
- Scientific Literature Resources
- Research Paper Management Tools
- Molecular Biology Software
- Primer Design Tools
- Bioinformatics Tools
- Phylogenetic Tree Analysis
Customer Reviews
Have you used Anti-TIM-3 HAVCR2 Antibody?
Submit a review and receive an Amazon gift card.
- $30 for a review with an image
0 Reviews For Anti-TIM-3 HAVCR2 Antibody
Customer Q&As
Have a question?
Find answers in Q&As, reviews.
Can't find your answer?
Submit your question
