Anti-TIM-3 HAVCR2 Antibody

Product Name

Anti-TIM-3 HAVCR2 Antibody

View all TIM-3 Antibodies

SKU/Catalog Number

A00657

Size

0.1 mg

Form

Liquid

Description

Boster Bio Anti-TIM-3 HAVCR2 Antibody (Catalog # A00657). Tested in ELISA, WB, IHC-P, IF applications. This antibody reacts with Human.

Storage & Handling

TIM-3 antibody can be stored at 4°C for three months and -20°C, stable for up to one year. Avoid repeated freeze-thaw cycles. Antibodies should not be exposed to prolonged high temperatures.

Cite This Product

Anti-TIM-3 HAVCR2 Antibody (Boster Biological Technology, Pleasanton CA, USA, Catalog # A00657)

Host

Rabbit

Contents

TIM-3 Antibody is supplied in PBS containing 0.02% sodium azide.

Clonality

Polyclonal

Isotype

IgG

Immunogen

TIM-3 antibody was raised against a peptide corresponding to 16 amino acids near the amino terminus of human TIM-3. The immunogen is located within amino acids 60 - 110 of TIM-3.

*Blocking peptide can be purchased. Costs vary based on immunogen length. Contact us for pricing.

Cross-reactivity

At least two antibodies are known to exist; this antibody will detect both isoforms.

Reactive Species

A00657 is reactive to HAVCR2 in Human

Applications

A00657 is guaranteed for ELISA, IF, IHC-P, WB Boster Guarantee

Observed Molecular Weight

68 kDa

Calculated molecular weight

33394 MW

Background of TIM-3

The immune checkpoint protein TIM-3 is a member of the immunoglobulin superfamily and TIM family of proteins that was initially identified as a specific marker of fully differentiated IFN-γ producing CD4 T helper 1 (Th1) and CD8 cytotoxic cells. It is a Th1-specific cell surface protein that regulates macrophage activation and negatively regulates Th1-mediated auto- and alloimmune responses, and is also highly expressed on regulatory T cells, monocytes, macrophages, and dendritic cells (1). TIM-3 and PD-1 are co-expressed on most CD4 and CD8 T cells infiltrating solid tumors or in hematologic malignancy in mice; blocking TIM-3 in conjugation with a PD-1 blockade increases the functionality of exhausted T cells and synergizes with to inhibit tumor growth (2,3).

Antibody Validation

Boster validates all antibodies on WB, IHC, ICC, Immunofluorescence, and ELISA with known positive control and negative samples to ensure specificity and high affinity, including thorough antibody incubations.

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Assay Dilutions Recommendation

The recommendations below provide a starting point for assay optimization. The actual working concentration varies and should be decided by the user.

TIM-3 antibody can be used for Western blot at 1 - 2 μg/mL. Antibody can also be used for Immunohistochemistry at 2 μg/mL. For Immunoflorescence start at 20 μg/mL.
Antibody validated: Western Blot in human samples; Immunohistochemistry in human samples and Immunofluorescence in human samples. All other applications and species not yet tested. Optimal dilutions for each application should be determined by the researcher.

Validation Images & Assay Conditions

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Western blot analysis of TIM-3 in Jurkat cell lysate with TIM-3 antibody at 1 μg/mL in (A) the absence and (B) the presence of blocking peptide.

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Immunohistochemistry of TIM-3 in human spleen tissue with TIM-3 antibody at 2 μg/ml.

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Immunofluorescence of TIM-3 in human spleen tissue with TIM-3 antibody at 20 μg/ml.

Red: TIM-3 Antibody (A00657)
Blue: DAPI staining

Gene/Protein Information For HAVCR2 (Source: Uniprot.org, NCBI)

Alternative Names

CD366; FLJ14428; HAVCR2; HAVcr-2; hepatitis A virus cellular receptor 2; kidney injury molecule-3; KIM-3; SPTCL; T cell immunoglobulin mucin 3; T cell immunoglobulin mucin-3; T-cell immunoglobulin and mucin domain-containing protein 3; TIM 3; TIM3 T-cell membrane protein 3; TIM3; TIM-3; TIMD3; TIMD-3; TIMD3KIM-3 HAVCR2 CD366, HAVcr-2, KIM-3, SPTCL, TIM3, TIMD-3, TIMD3, Tim-3 hepatitis A virus cellular receptor 2 hepatitis A virus cellular receptor 2|T cell immunoglobulin mucin 3|T-cell immunoglobulin and mucin domain-containing protein 3|T-cell immunoglobulin mucin family member 3|T-cell immunoglobulin mucin receptor 3|T-cell membrane protein 3|kidney injury molecule-3

For more info on HAVCR2, check out the HAVCR2 Infographic

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In this infographic, you will see the following information for HAVCR2: database IDs, superfamily, protein function, synonyms, molecular weight, chromosomal locations, tissues of expression, subcellular locations, post-translational modifications, and related diseases, research areas & pathways. If you want to see more information included, or would like to contribute to it and be acknowledged, please contact [email protected].

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