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Product Info Summary
| SKU: | A04320-2 |
|---|---|
| Size: | 100ug |
| Reactive Species: | Human |
| Host: | Sheep |
| Application: | WB |
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Product info
Product Name
Anti-Thymidylate Synthase Antibody
View all Thymidylate Synthase Antibodies
SKU/Catalog Number
A04320-2
Size
100ug
Form
Lyophilized
Description
Boster Bio Anti-Thymidylate Synthase Antibody (Catalog # A04320-2). Tested in WB applications. This antibody reacts with Human.
Storage & Handling
Store vial at 4°C prior to restoration. For extended storage aliquot contents and freeze at -20°C or below. Avoid cycles of freezing and thawing. Centrifuge product if not completely clear after standing at room temperature. This product is stable for several weeks at 4°C as an undiluted liquid. Dilute only prior to immediate use.?Expiration date is one (1) year from date of opening. (Ship on dry ice.)
Cite This Product
Anti-Thymidylate Synthase Antibody (Boster Biological Technology, Pleasanton CA, USA, Catalog # A04320-2)
Host
Sheep
Contents
0.02 M Potassium Phosphate, 0.15 M Sodium Chloride, pH 7.2, 0.01% (w/v) Sodium Azide
Clonality
Polyclonal
Isotype
IgG
Immunogen
Anti-THYMIDYLATE SYNTHASE was purified from rabbit serum after repeated immunizations with recombinant human Thymidylate Synthase (36 kDa) produced in E.coli.
*Blocking peptide can be purchased. Costs vary based on immunogen length. Contact us for pricing.
Reactive Species
A04320-2 is reactive to TYMS in Human
Reconstitution
Restore with deionized water (or equivalent) for reconstitution volume of 100 µL
Calculated molecular weight
35.716kDa
Background of Thymidylate Synthase
Thymidylate synthase catalyzes the methylation of deoxyuridylate to deoxythymidylate using 5,10-methylenetetrahydrofolate (methylene-THF) as a cofactor. This function maintains the dTMP (thymidine-5-prime monophosphate) pool critical for DNA replication and repair. The enzyme has been of interest as a target for cancer chemotherapeutic agents. It is considered to be the primary site of action for 5-fluorouracil, 5-fluoro-2-prime-deoxyuridine, and some folate analogs. Expression of this gene and that of a naturally occurring antisense transcript rTSalpha vary inversely when cell-growth progresses from late-log to plateau phase. Diseases associated with Thymidylate synthase include Rectal Neoplasm and Dihydropyrimidine Dehydrogenase Deficiency. Anti-Thymidylate synthase is useful for researchers interested in Circadian Rhythm, Metabolism and cell cycle research.
Antibody Validation
Boster validates all antibodies on WB, IHC, ICC, Immunofluorescence, and ELISA with known positive control and negative samples to ensure specificity and high affinity, including thorough antibody incubations.
Application & Images
Applications
A04320-2 is guaranteed for WB Boster Guarantee
Assay Dilutions Recommendation
The recommendations below provide a starting point for assay optimization. The actual working concentration varies and should be decided by the user.
ELISA: 1:10,000 - 1:50,000
IHC: User optimized
IF Microscopy: User optimized
WB: 1:500 - 1:2,000
Validation Images & Assay Conditions
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Anti-TS is shown to detect thymidylate synthase present in a HeLa cell extract.Each lane is estimated to contain 4 ug of protein.Lanes 1, 2 and 3 represent 1:2,000, 1:5,000 and 1:10,000 fold dilutions of the antibody.Detection was made using HRP Rabbit-a-Sheep IgG (613-4302) diluted 1:1,000 and color development using TMB (TMBM-100) substrate for approximately 4'.
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Figure 2. Western blot analysis of TYMS using anti-TYMS antibody (A04320-2).
Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel) / 90V (Resolving gel) for 2-3 hours. The sample well of each lane was loaded with 50ug of sample under reducing conditions.
After Electrophoresis, proteins were transferred to a Nitrocellulose membrane at 150mA for 50-90 minutes. Blocked the membrane with 5% Non-fat Milk/ TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-TYMS antigen affinity purified polyclonal antibody (Catalog # A04320-2) at 0.5 ug/mL overnight at 4°C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-Sheep IgG-HRP secondary antibody at a dilution of 1:10000 for 1.5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit (Catalog # SA1023) with Tanon 5200 system. A specific band was detected for TYMS.
Protein Target Info & Infographic
Gene/Protein Information For TYMS (Source: Uniprot.org, NCBI)
Gene Name
TYMS
Full Name
Thymidylate synthase
Weight
35.716kDa
Superfamily
thymidylate synthase family
Alternative Names
Thymidylate Synthase, d TMP synthase, EC 2.1.1.45, HsT422, MGC88736 TYMS HST422, TMS, TS thymidylate synthetase thymidylate synthase|TSase
*If product is indicated to react with multiple species, protein info is based on the gene entry specified above in "Species".For more info on TYMS, check out the TYMS Infographic
We have 30,000+ of these available, one for each gene! Check them out.
In this infographic, you will see the following information for TYMS: database IDs, superfamily, protein function, synonyms, molecular weight, chromosomal locations, tissues of expression, subcellular locations, post-translational modifications, and related diseases, research areas & pathways. If you want to see more information included, or would like to contribute to it and be acknowledged, please contact [email protected].
Specific Publications For Anti-Thymidylate Synthase Antibody (A04320-2)
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