Product Info Summary
SKU: | A02765-4 |
---|---|
Size: | 100 μg/vial |
Reactive Species: | Human, Rat |
Host: | Rabbit |
Application: | WB |
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Product info
Product Name
Anti-TGF beta Receptor III/TGFBR3 Antibody Picoband™
View all TGF-beta RIII Antibodies
SKU/Catalog Number
A02765-4
Size
100 μg/vial
Form
Lyophilized
Description
Boster Bio Anti-TGF beta Receptor III/TGFBR3 Antibody Picoband™ catalog # A02765-4. Tested in WB applications. This antibody reacts with Human, Rat.
Storage & Handling
At -20°C for one year from date of receipt. After reconstitution, at 4°C for one month. It can also be aliquotted and stored frozen at -20°C for six months. Avoid repeated freezing and thawing.
Cite This Product
Anti-TGF beta Receptor III/TGFBR3 Antibody Picoband™ (Boster Biological Technology, Pleasanton CA, USA, Catalog # A02765-4)
Host
Rabbit
Contents
Each vial contains 4 mg Trehalose, 0.9 mg NaCl, 0.2 mg Na2HPO4.
Clonality
Polyclonal
Isotype
Rabbit IgG
Immunogen
A synthetic peptide corresponding to a sequence at the C-terminus of human TGF beta Receptor III/TGFBR3, which shares 92.3% amino acid (aa) sequence identity with mouse and rat TGFBR3.
*Blocking peptide can be purchased. Costs vary based on immunogen length. Contact us for pricing.
Cross-reactivity
No cross-reactivity with other proteins.
Reactive Species
A02765-4 is reactive to TGFBR3 in Human, Rat
Applications
A02765-4 is guaranteed for WB Boster Guarantee
Observed Molecular Weight
93 kDa
Calculated molecular weight
12252 MW
Background of TGF-beta RIII
Betaglycan also known as Transforming growth factor beta receptor III (TGFBR3), is a cell-surface chondroitin sulfate / heparan sulfate proteoglycan >300 kDa in molecular weight. This locus encodes the transforming growth factor (TGF)-beta type III receptor. The encoded receptor is a membrane proteoglycan that often functions as a co-receptor with other TGF-beta receptor superfamily members. Ectodomain shedding produces soluble TGFBR3, which may inhibit TGFB signaling. Decreased expression of this receptor has been observed in various cancers. Alternatively spliced transcript variants encoding different isoforms have been identified for this gene.
Antibody Validation
Boster validates all antibodies on WB, IHC, ICC, Immunofluorescence, and ELISA with known positive control and negative samples to ensure specificity and high affinity, including thorough antibody incubations.
Assay dilution & Images
Reconstitution
Adding 0.2 ml of distilled water will yield a concentration of 500 μg/ml.
Assay Dilutions Recommendation
The recommendations below provide a starting point for assay optimization. The actual working concentration varies and should be decided by the user.
Western blot, 0.1-0.25 μg/ml, Human, Rat
Validation Images & Assay Conditions
![a02765 4 tgfbr3 primary antibodies wb testing 1 a02765 4 tgfbr3 primary antibodies wb testing 1](https://www.bosterbio.com/media/catalog/product/a/0/a02765-4-tgfbr3-primary-antibodies-wb-testing-1.jpg)
Click image to see more details
Figure 1. Western blot analysis of TGF Beta Receptor III/TGFBR3 using anti-TGF Beta Receptor III/TGFBR3 antibody (A02765-4).
Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel) / 90V (Resolving gel) for 2-3 hours. The sample well of each lane was loaded with 30 ug of sample under reducing conditions.
Lane 1: human K562 whole cell lysates,
Lane 2: human Hela whole cell lysates,
Lane 3: rat testis tissue lysates,
Lane 4: rat L6 whole cell lysates.
After electrophoresis, proteins were transferred to a nitrocellulose membrane at 150 mA for 50-90 minutes. Blocked the membrane with 5% non-fat milk/TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-TGF Beta Receptor III/TGFBR3 antigen affinity purified polyclonal antibody (Catalog # A02765-4) at 0.25 μg/mL overnight at 4°C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:5000 for 1.5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit (Catalog # EK1002) with Tanon 5200 system. A specific band was detected for TGF Beta Receptor III/TGFBR3 at approximately 93 kDa. The expected band size for TGF Beta Receptor III/TGFBR3 is at 93 kDa.
Protein Target Info & Infographic
Gene/Protein Information For TGFBR3 (Source: Uniprot.org, NCBI)
Gene Name
TGFBR3
Full Name
Transforming growth factor beta receptor type 3
Weight
12252 MW
Alternative Names
betaglycan proteoglycan; Betaglycan; BGCAN; TBRIII; TGF-beta receptor type 3; TGF-beta receptor type III; TGF-beta RIII; TGFbetaRIII; TGFBR3; TGF-bRIII; TGFR-3; Transforming growth factor beta receptor III; transforming growth factor beta receptor type 3; transforming growth factor, beta receptor III (betaglycan, 300kDa); transforming growth factor, beta receptor III TGFBR3 BGCAN, betaglycan transforming growth factor beta receptor 3 transforming growth factor beta receptor type 3|TGF-beta receptor type 3|TGF-beta receptor type III|TGFR-3|betaglycan proteoglycan|transforming growth factor beta receptor III
*If product is indicated to react with multiple species, protein info is based on the gene entry specified above in "Species".For more info on TGFBR3, check out the TGFBR3 Infographic
![TGFBR3 infographic](/media/images/gene-infographic-example.jpg)
We have 30,000+ of these available, one for each gene! Check them out.
In this infographic, you will see the following information for TGFBR3: database IDs, superfamily, protein function, synonyms, molecular weight, chromosomal locations, tissues of expression, subcellular locations, post-translational modifications, and related diseases, research areas & pathways. If you want to see more information included, or would like to contribute to it and be acknowledged, please contact [email protected].
Specific Publications For Anti-TGF beta Receptor III/TGFBR3 Antibody Picoband™ (A02765-4)
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