Anti-TGF-alpha (Transforming Growth Factor alpha) Monoclonal Antibody

Product Name

Anti-TGF-alpha (Transforming Growth Factor alpha) Monoclonal Antibody

View all TGF-alpha Antibodies

SKU/Catalog Number

M01779

Size

100ug/vial

Form

Liquid

Description

Boster Bio Anti-TGF-alpha (Transforming Growth Factor alpha) Monoclonal Antibody (Catalog # M01779). Tested in Flow Cytometry, IF, IHC applications. This antibody reacts with Human, Rabbit, Zebrafish.

Storage & Handling

Antibody with azide - store at 2 to 8°C. Antibody without azide - store at -20 to -80°C. Antibody is stable for 24 months. Non-hazardous. No MSDS required.

Cite This Product

Anti-TGF-alpha (Transforming Growth Factor alpha) Monoclonal Antibody (Boster Biological Technology, Pleasanton CA, USA, Catalog # M01779)

Host

Mouse

Contents

Prepared in 10mM PBS with 0.05% BSA & 0.05% azide. Also available WITHOUT BSA at 1.0mg/ml.

Clonality

Monoclonal

Clone Number

Clone: TG86 + P/T1

Isotype

IgG1, kappa + IgG1, kappa

Immunogen

A 10-amino acid synthetic peptide (aa34-43; PPVAAAVVSH) from human TGFα (TG86 & P/T1)

*Blocking peptide can be purchased. Costs vary based on immunogen length. Contact us for pricing.

Cross-reactivity

Does not cross-react with primate, avian or amphibian GR.

Reactive Species

M01779 is reactive to TGFA in Human, Rabbit, Zebrafish

Reconstitution

Reconstitute with distilled water.

Observed Molecular Weight

68 kDa

Calculated molecular weight

17006 MW

Background of TGF-alpha

This antibody reacts with the TGF alpha and shows no cross-reaction with EGF and the neuropeptide synenkephalin. The staining with This monoclonal antibody is completely blocked by the peptide used for raising this antibody. TGF (aa50) is a growth factor with 33% homology to EGF, binds to EGFR, activates tyrosine phosphorylation of the receptor, and stimulates cell proliferation. It plays a role in tumor initiation by inducing the reversible transformed phenotype.

Antibody Validation

Boster validates all antibodies on WB, IHC, ICC, Immunofluorescence, and ELISA with known positive control and negative samples to ensure specificity and high affinity, including thorough antibody incubations.

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Applications

M01779 is guaranteed for Flow Cytometry, IF, IHC Boster Guarantee

Assay Dilutions Recommendation

The recommendations below provide a starting point for assay optimization. The actual working concentration varies and should be decided by the user.

Flow Cytometry (1-2ug/million cells)
Immunofluorescence (1-2ug/ml)
Immunohistochemistry (Formalin-fixed) (2-4ug/ml for 30 min at RT)(Staining of formalin-fixed tissues requires heating tissue sections in 10mM Tris with 1mM EDTA, pH 9.0, for 45 min at 95°C followed by cooling at RT for 20 minutes)
Optimal dilution for a specific application should be determined.

Validation Images & Assay Conditions

Gene/Protein Information For TGFA (Source: Uniprot.org, NCBI)

Alternative Names

Protransforming growth factor alpha;Transforming growth factor alpha;TGF-alpha;EGF-like TGF;ETGF;TGF type 1;TGFA; TGFA TFGA transforming growth factor alpha protransforming growth factor alpha|TGF-alpha

*If product is indicated to react with multiple species, protein info is based on the gene entry specified above in "Species".

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