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Facts about Protransforming growth factor alpha.
Human | |
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Gene Name: | TGFA |
Uniprot: | P01135 |
Entrez: | 7039 |
Belongs to: |
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No superfamily |
protransforming growth factor alpha; TFGA; TGFA; TGFalpha; TGF-alpha; transforming growth factor, alpha; transforming growth factor-alpha
Mass (kDA):
17.006 kDA
Human | |
---|---|
Location: | 2p13.3 |
Sequence: | 2; NC_000002.12 (70447284..70553826, complement) |
[Transforming growth factor alpha]: Secreted, extracellular space.; [Protransforming growth factor alpha]: Cell membrane; Single-pass type I membrane protein.
This Boster Bio article will cover the TGFA marker as well as High-affinity prima antibodies. This article will give you an excellent overview of the protein. Please read the rest to learn more about this particular protein. Then, you can choose the TGFA marker for your own research. If you have questions or comments, please let us know.
Steven Boster's most valuable tool is the TGFA mark. It allows him quick access to his inventory. Boster used TGFA markers to locate items. Other TGFA members were able find him by scanning barcodes. The following are Boster’s favorite uses of the marker.
Boster Bio provides high-affinity rabbit MAbs. Its PCD platform produces high-affinity clones for Diagnostics & Therapeutics applications. This proprietary technology utilizes flow cytometry with proprietary chemistry to prevent the secretion and retention of antibodies by the B cells. Flurochrome conjugated antibodies are incubated in the splenocytes. Clones with the highest brightness can be isolated for downstream screening.
The company has twenty years of experience in antibody manufacturing. Their products are sensitive, specific and stable. They have also been rigorously validated. Over 14,000 scientists trust the Boster Picokine(tm), ELISA kits. These kits are based upon high-affinity primary antibodies. They can detect native forms proteins with subpicogram or picogram levels of sensitivity. The boster antibody makes flow cytometry a very easy task.
The enzyme-linked immunosorbent technology (ELISA), is similar to other immunoassay techniques. Both systems use specific antibodies to bind the target antibody and a detection system to indicate binding. High-affinity antibody are essential to maximize the assay's sensitiveness. Boster Bio is an expert in coating plates with high-affinity antibody. Boster Bio high affinity antibodies are trusted by scientists all over the globe.
Dual-labeling specimens is another advantage. Dual labeling of specimens allows researchers to ask more questions and gain more context. Secondary antibodies can also be conjugated with different compounds. This allows researchers from different backgrounds to compare the prevalence of specimens. The dual-labeling system also helps researchers get more information on the results of their experiments.
The final deliverables from the process are antibody sequencing, which are essential to reproducible results. Recombinant antibodies are also able to preserve genetic information. Customers can choose the three most desirable clones from their supernatant. In return, they will receive ELISA information, a map showing the vector, purified antibodies, and 100 ng of heavy strand plasmid genetic data. It takes only a few months, but it is worth the effort.
Monoclonal antibodies work best for native proteins. Polyclonal ones don't perform well with denatured proteins. WB results can't be relied on as a guarantee that monoclonal antibodies are specific. In addition to WB, researchers can also use IHC or Flow cytometry to further validate the results of monoclonal antibodies. Negative controls can be used in both cases to detect false positives and false-positives that could affect test results.
CL/P risk has been linked with the TGFA polymorphism. Ardinger and colleagues found a link between Taq I mutations in the TGFA locus as well as the risk of developing the disorder. Numerous studies have repeated the association since then. Moreover, several studies have linked the TGFA polymorphism to CL/P. However, further research is needed in order to confirm this association.
These studies sought to confirm the role played by TGFA in the onset of hypertensive and chronic kidney disease. We used neutralizing antisera (NA) as an assay, and an adenoassociatedvirus approach (AAV), in order to overexpress TGFA within the serum. This allowed for a direct comparison of the TGFA levels between the serum samples and urine of both groups.
PMID: 6088071 by Derynck R., et al. Human transforming growth factor-alpha: precursor structure and expression in E. coli.
PMID: 2464748 by Jakowlew S.B., et al. A novel low molecular weight ribonucleic acid (RNA) related to transforming growth factor alpha messenger RNA.
*Showing only the more recent 20. More publications can be found for each product on its corresponding product page