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Product Info Summary
| SKU: | PA1248 |
|---|---|
| Size: | 100 μg/vial |
| Reactive Species: | Human |
| Host: | Rabbit |
| Application: | IHC, WB |
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Product info
Product Name
Anti-TFPI2 Antibody Picoband®
SKU/Catalog Number
PA1248
Size
100 μg/vial
Form
Lyophilized
Description
Boster Bio Anti-TFPI2 Antibody catalog # PA1248. Tested in IHC, WB applications. This antibody reacts with Human. The brand Picoband indicates this is a premium antibody that guarantees superior quality, high affinity, and strong signals with minimal background in Western blot applications. Only our best-performing antibodies are designated as Picoband, ensuring unmatched performance.
Storage & Handling
Store at -20˚C for one year from date of receipt. After reconstitution, at 4˚C for one month. It can also be aliquotted and stored frozen at -20˚C for six months. Avoid repeated freeze-thaw cycles.
Cite This Product
Anti-TFPI2 Antibody Picoband® (Boster Biological Technology, Pleasanton CA, USA, Catalog # PA1248)
Host
Rabbit
Contents
Each vial contains 5mg BSA, 0.9mg NaCl, 0.2mg Na2HPO4, 0.05mg Thimerosal, 0.05mg NaN3.
Clonality
Polyclonal
Isotype
Rabbit IgG
Immunogen
A synthetic peptide corresponding to a sequence in the middle region of human TFPI2.
*Blocking peptide can be purchased. Costs vary based on immunogen length. Contact us for pricing.
Cross-reactivity
No cross-reactivity with other proteins
Reactive Species
PA1248 is reactive to TFPI2 in Human
Reconstitution
Add 0.2ml of distilled water will yield a concentration of 500ug/ml.
Observed Molecular Weight
51 kDa
Calculated molecular weight
26934 MW
Background of TFPI-2
Tissue factor pathway inhibitor 2, also known as TFPI2, is a human gene which is located at 7q22. It is an important regulator of the extrinsic pathway of blood coagulation through its ability to inhibit factor Xa and factor VIIa-tissue factor activity. After a 22-residue signal peptide, the mature TFPI2 protein contains 213 amino acids with 18 cysteines and 2 canonical N-linked glycosylation sites. The purified recombinant TFPI2 strongly inhibited the amidolytic activities of trypsin and the factor VIIa-tissue factor complex. The latter inhibition was markedly enhanced in the presence of heparin. Mouse TFPI2 mRNA is highly expressed in developing mouse placenta, as in human. And there are also high transcript levels in adult mouse liver and kidney.
Antibody Validation
Boster validates all antibodies on WB, IHC, ICC, Immunofluorescence, and ELISA with known positive control and negative samples to ensure specificity and high affinity, including thorough antibody incubations.
Application & Images
Applications
PA1248 is guaranteed for IHC, WB Boster Guarantee
Assay Dilutions Recommendation
The recommendations below provide a starting point for assay optimization. The actual working concentration varies and should be decided by the user.
Immunohistochemistry (Paraffin-embedded Section), 0.5-1μg/ml, Human, By Heat
Western blot, 0.1-0.5μg/ml, Human
Positive Control
WB: MM453 Cell, MM231 Cell, HELA Cell, HT1080 Cell, JURKAT Cell
IHC: Human Mammary Cancer Tissue
Validation Images & Assay Conditions
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Anti-TFPI2 antibody, PA1248, Western blotting
Lane 1: MM453 Cell Lysate
Lane 2: MM231 Cell Lysate
Lane 3: HELA Cell Lysate
Lane 4: HT1080 Cell Lysate
Lane 5: JURKAT Cell Lysate
Click image to see more details
Anti-TFPI2 antibody, PA1248, IHC(P)
IHC(P): Human Mammary Cancer Tissue
Protein Target Info & Infographic
Gene/Protein Information For TFPI2 (Source: Uniprot.org, NCBI)
Gene Name
TFPI2
Full Name
Tissue factor pathway inhibitor 2
Weight
26934 MW
Alternative Names
Tissue factor pathway inhibitor 2;TFPI-2;Placental protein 5;PP5;TFPI2; TFPI2 PP5, REF1, TFPI-2 tissue factor pathway inhibitor 2 tissue factor pathway inhibitor 2|placental protein 5|retinal pigment epithelium cell factor 1
*If product is indicated to react with multiple species, protein info is based on the gene entry specified above in "Species".For more info on TFPI2, check out the TFPI2 Infographic
We have 30,000+ of these available, one for each gene! Check them out.
In this infographic, you will see the following information for TFPI2: database IDs, superfamily, protein function, synonyms, molecular weight, chromosomal locations, tissues of expression, subcellular locations, post-translational modifications, and related diseases, research areas & pathways. If you want to see more information included, or would like to contribute to it and be acknowledged, please contact [email protected].
Specific Publications For Anti-TFPI2 Antibody Picoband® (PA1248)
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No publications found for PA1248
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Customer Reviews
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Customer Q&As
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1 Customer Q&As for Anti-TFPI2 Antibody Picoband®
Question
We are currently using anti-TFPI2 antibody PA1248 for human tissue, and we are satisfied with the WB results. The species of reactivity given in the datasheet says human. Is it true that the antibody can work on primate tissues as well?
M. Bhatt
Verified customer
Asked: 2013-10-30
Answer
The anti-TFPI2 antibody (PA1248) has not been tested for cross reactivity specifically with primate tissues, but there is a good chance of cross reactivity. We have an innovator award program that if you test this antibody and show it works in primate you can get your next antibody for free. Please contact me if I can help you with anything.
Boster Scientific Support
Answered: 2013-10-30
