Product Info Summary
SKU: | A05158 |
---|---|
Size: | 100 µl |
Reactive Species: | Human, Mouse |
Host: | Rabbit |
Application: | IF, IHC, WB |
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Product info
Product Name
Anti-TFIP8 TNFAIP8 Antibody
SKU/Catalog Number
A05158
Size
100 µl
Form
Liquid
Description
Boster Bio Anti-TFIP8 TNFAIP8 Antibody (Catalog # A05158). Tested in WB, IHC, IF applications. This antibody reacts with Human, Mouse.
Storage & Handling
Store at -20°C for one year. For short term storage and frequent use, store at 4°C for up to one month. Avoid repeated freeze-thaw cycles.
Cite This Product
Anti-TFIP8 TNFAIP8 Antibody (Boster Biological Technology, Pleasanton CA, USA, Catalog # A05158)
Host
Rabbit
Contents
Rabbit IgG in phosphate buffered saline (without Mg2+ and Ca2+), pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol.
Clonality
Polyclonal
Isotype
IgG
Immunogen
Synthesized peptide derived from internal of human TFIP8.
*Blocking peptide can be purchased. Costs vary based on immunogen length. Contact us for pricing.
Reactive Species
A05158 is reactive to TNFAIP8 in Human, Mouse
Reconstitution
Observed Molecular Weight
111 kDa
Calculated molecular weight
23003 MW
Background of TNFAIP8
Acts as a negative mediator of apoptosis and may play a role in tumor progression. Suppresses the TNF-mediated apoptosis by inhibiting caspase-8 activity but not the processing of procaspase-8, subsequently resulting in inhibition of BID cleavage and caspase-3 activation.
Horrevoets A.J.G., Blood 93:3418-3431(1999).
Kumar D., J. Biol. Chem. 275:2973-2978(2000).
You Z., J. Biol. Chem. 276:26398-26404(2001).
Antibody Validation
Boster validates all antibodies on WB, IHC, ICC, Immunofluorescence, and ELISA with known positive control and negative samples to ensure specificity and high affinity, including thorough antibody incubations.
Application & Images
Applications
A05158 is guaranteed for IF, IHC, WB Boster Guarantee
Assay Dilutions Recommendation
The recommendations below provide a starting point for assay optimization. The actual working concentration varies and should be decided by the user.
Western blotting: 1:500~1:3000
Immunohistochemistry: 1:50~1:100
Immunofluorescence: 1:100~1:500
Validation Images & Assay Conditions
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Immunohistochemistry analysis of paraffin-embedded human lung carcinoma tissue using TFIP8 antibody A05158.
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Western blot analysis of extracts from HUVEC cells, using TFIP8 antibody A05158.
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Immunofluorescence analysis of A549 cells, using TFIP8 antibody A05158.
Protein Target Info & Infographic
Gene/Protein Information For TNFAIP8 (Source: Uniprot.org, NCBI)
Gene Name
TNFAIP8
Full Name
Tumor necrosis factor alpha-induced protein 8
Weight
23003 MW
Superfamily
TNFAIP8 family
Alternative Names
Tumor necrosis factor alpha-induced protein 8;TNF alpha-induced protein 8;Head and neck tumor and metastasis-related protein;MDC-3.13;NF-kappa-B-inducible DED-containing protein;NDED;SCC-S2;TNF-induced protein GG2-1;TNFAIP8; TNFAIP8 GG2-1, MDC-3.13, NDED, SCC-S2, SCCS2 TNF alpha induced protein 8 tumor necrosis factor alpha-induced protein 8|NF-kappa-B-inducible DED-containing protein|TNF-induced protein GG2-1|head and neck tumor and metastasis-related protein|tumor necrosis factor, alpha induced protein 8
*If product is indicated to react with multiple species, protein info is based on the gene entry specified above in "Species".For more info on TNFAIP8, check out the TNFAIP8 Infographic
We have 30,000+ of these available, one for each gene! Check them out.
In this infographic, you will see the following information for TNFAIP8: database IDs, superfamily, protein function, synonyms, molecular weight, chromosomal locations, tissues of expression, subcellular locations, post-translational modifications, and related diseases, research areas & pathways. If you want to see more information included, or would like to contribute to it and be acknowledged, please contact [email protected].
Specific Publications For Anti-TFIP8 TNFAIP8 Antibody (A05158)
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Customer Q&As
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4 Customer Q&As for Anti-TFIP8 TNFAIP8 Antibody
Question
I was wanting to use your anti-TFIP8 antibody for IHC for mouse brain urinary bladder on frozen tissues, but I want to know if it has been validated for this particular application. Has this antibody been validated and is this antibody a good choice for mouse brain urinary bladder identification?
Verified Customer
Verified customer
Asked: 2019-07-11
Answer
As indicated on the product datasheet, A05158 anti-TFIP8 antibody has been validated for IF, IHC, WB on human, mouse tissues. We have an innovator award program that if you test this antibody and show it works in mouse brain urinary bladder in IHC-frozen, you can get your next antibody for free.
Boster Scientific Support
Answered: 2019-07-11
Question
We are currently using anti-TFIP8 antibody A05158 for mouse tissue, and we are satisfied with the IHC results. The species of reactivity given in the datasheet says human, mouse. Is it possible that the antibody can work on feline tissues as well?
D. Zhang
Verified customer
Asked: 2019-02-26
Answer
The anti-TFIP8 antibody (A05158) has not been tested for cross reactivity specifically with feline tissues, though there is a good chance of cross reactivity. We have an innovator award program that if you test this antibody and show it works in feline you can get your next antibody for free. Please contact me if I can help you with anything.
Boster Scientific Support
Answered: 2019-02-26
Question
Is there a BSA free version of anti-TFIP8 antibody A05158 available?
Verified Customer
Verified customer
Asked: 2018-08-28
Answer
We appreciate your recent telephone inquiry. I can confirm that some lots of this anti-TFIP8 antibody A05158 are BSA free. For now, these lots are available and we can make a BSA free formula for you free of charge. It will take 3 extra days to prepare. If you require this antibody BSA free again in future, please do not hesitate to contact me and I will be pleased to check which lots we have in stock that are BSA free.
Boster Scientific Support
Answered: 2018-08-28
Question
Is a blocking peptide available for product anti-TFIP8 antibody (A05158)?
Verified Customer
Verified customer
Asked: 2017-11-30
Answer
We do provide the blocking peptide for product anti-TFIP8 antibody (A05158). If you would like to place an order for it please contact [email protected] and make a special request.
Boster Scientific Support
Answered: 2017-11-30