Tetraspanin-8 Antibodies

Tetraspanin-8 (TSPAN8)

Gene Information

Human
Gene Name:	TSPAN8
Uniprot:	P19075
Entrez:	7103

Family

Belongs to:
tetraspanin (TM4SF) family

Alternative Names

CO-029; tetraspanin 8; TM4SF3; TM4SF3tspan-8; Transmembrane 4 superfamily member 3tetraspanin-8; TSPAN8; Tspan-8; Tumor-associated antigen CO-029

Molecular Weight

Mass (kDA):
26.044 kDA

Genomic Context

Human
Location:	12q21.1
Sequence:	12; NC_000012.12 (71125093..71157999, complement)

Tissue Specificity

Gastric, colon, rectal, and pancreatic carcinomas.

Subcellular Localizations

Membrane; Multi-pass membrane protein.

Diseases

• Diabetes Mellitus
• Diabetes Mellitus, Non-insulin-dependent
• Neoplasms
• Malignant Neoplasms
• Neoplasm Metastasis
• Adenocarcinoma
• Insulin Resistance
• Insulin Sensitivity
• Carcinoma
• Tumor Progression
• Cell Invasion
• Tissue Adhesions

• Obesity
• Pancreatic Neoplasm
• Pathologic Neovascularization
• Colorectal Cancer
• Malignant Tumor Of Colon
• Colorectal Neoplasms
• Liver Neoplasms

Interacting Proteins

• MEP1B

Pathways

• Secretion
• Insulin Secretion
• Cell Migration
• Cell Proliferation
• Cell Motility
• Pathogenesis
• Angiogenesis
• Cell Adhesion
• Regeneration
• Coagulation
• Cell Cycle
• Cell Growth
• Cell Activation

• Endothelial Cell Activation
• Cell-matrix Adhesion
• Oxidative Phosphorylation
• Liver Regeneration
• Calcium-dependent Cell-cell Adhesion
• Rna Interference
• Cell-cell Adhesion

PTMs

• Phosphorylation

Research Areas

• Cancer

For details, visit:
bosterbio.com/bosterbio-gene-info-cards/TSPAN8

Best Uses Of The TSPAN8 Marker

In this article, you'll learn about TSPAN8, a new therapeutic target for monoclonal antibodies. This protein regulates leukocyte transport and mediates survival, proliferation and metastasis in colon cancer cells. It also regulates integrin-surface expression and acts as regulator of leukocyte circulation. This makes TSPAN8 exciting for research.

TSPAN8 is an emerging therapeutic target in monoclonal antibody therapy

TSPAN8 is an epithelial protein found in many healthy tissues. It is also found in certain types of cancers. TSPAN8 expression is positively associated both with poor prognosis, and metastasis. Scientists have discovered multiple mechanisms of action for TSPAN8, which include cell adhesion and motility. Many monoclonal antibodies are targeting TSPAN8 with the hope that they will discover a new therapeutic target.

Mouse IgG2b antibodies to TSPAN8 were prepared from supernatant cultures of human and mouse hybridomas, and were purified using the previously described methods. TSPAN8 immunohistochemistry revealed that these antibodies inhibit the growth of tumor xenografts in nude mice. Further studies are underway to determine the effectiveness and safety of TSPAN8 monoclonal Antibody in Humans.

The antibody inhibits EGFR phosphorylation by disrupting the association between CD47 and EGFR. This antibody inhibits phosphorylation in EGFR cells by inhibiting the production of Exosomes. Additionally, B6h22-Ab also inhibits the phosphorylation of EGFR by blocking the interaction between CD47 and EGFR. B6h22Ab was also effective in inhibiting cell attachment, a process known "cell adhesion".

Effective therapies for cancer patients require careful targeting of genes that are involved with carcinogenesis and proteins that are important. Many genetic abnormalities are present in cancer cell lines, including the polycomb genes associated with tumors. The ubiquitous cell surface proteins CD47 are also associated with cancer cells. It has been shown to be associated with poor prognosis and can be found in stem cells from leukemic cancer.

It mediates survival, proliferation and metastasis for cancer cells

TSPAN8, a transmembrane proteins that controls the survival, proliferation and migration of cancer cells, is known as TSPAN8. It is highly expressed within various cancerous tissues. This protein has many biological functions, including adhesion to cells, cell migration, and invasion. TSPAN8 is a protein that can increase metastasis and proliferative potential in tumor cells.

TSPAN8 is a member in the tetraspanin Superfamily. It has four transmembrane domains (LEL) and two extracellular loops. Its amino- and carboxyterminal tails are short and well conserved. It has six conserved cysteine amino acids that form disulfide bonded that are essential to its LEL configuration.

TSPAN8 expression can also be associated with E-cadherin and epithelial cells adhesion molecules (ECAM), activity. It is unknown if Tspan8 promotes tumor cells' self-sufficiency when cultured in conventional cell cultures. It could also be involved in cell-cell adhesion.

TSPAN8 expression was associated in this study with higher levels in brain and liver of patients undergoing radiation therapy. Patients with a CEA of 5 ng/mL were more likely to express the protein than patients with a stage III+ IV TNM tumor. Although preliminary data are available, these results are compelling and warrant further investigation.

It decreases the sensitivity to chemotherapy agents of cancer cells

TSPAN8 and CD318 are two markers that may impair the sensitivity of cancer cells against chematherapeutic drugs. The CD318 marker, which has been found to be limited in expression in healthy tissues, has been confirmed. TSPAN8, a more controversial candidate for the CD318 marker, is detected in skeletal muscle as well as medulla.

TSPAN8 gene expression is upregulated in nonsmall cell lung cancer cells and normal human blood bronchial epithelial cellular cells. Overexpression of this protein increases viability and inhibits cell mortality. Most primary breast cancer lesions have high levels of TSPAN8 protein expression. TSPAN8 promotes cell adhesion and proliferation.

TSPAN8 is associated with CD9 and CD81 in pancreatic cancer cells. It also interacts with a2-b1 integrin and the E-cadherin/p120-catenin complex. It also interferes the regulation small GTPases. It can promote colorectal tumor progression and metastasis.

Its role in regulating the proliferation and invasion of cancer cells is not fully understood. The regulation of cancer stem cells is an important source of tumor resistance. TSPAN8 and CD proteins are key regulatory factors for cancer stem cells. They promote invasion, metastasis and migration when activated in cells.

It regulates leukocyte trafficking

TSPAN8 is a member the tetraspanin superfamily. It is highly expressed in many tissues including the gastrointestinal tract and large and small intestines as well as the reproductive organs. It contains two extracellular Loops, one small one that lies between TM1 & TM2, and three cytosolic Domains. It contains six conserved Cystine residues that form disulfide connections. These disulfide bonds are essential for LEL conformation.

The TSPAN8 marker is a promising new therapeutic target in radioimmunotherapy, and it has been shown to inhibit tumor growth in HT29 tumor-bearing mice. TSPAN8 antibodies, according to the company, have been shown to be effective against various TSPAN8 expression-related cancers, including melanomas, glioma as well as gastric cancer and aggressive CRC tumors.

Researchers are increasingly identifying TSPAN8’s role in tumor development and metastasis. This marker, which is a member in the tetraspanin suprafamily, regulates leukocyte transport, wound repair, angiogenesis, and other functions. This marker is associated with poor prognosis in many cancers.

It regulates angiogenesis

TSPAN8, a member of the tetraspanins family of integral membrane proteins, is a key player in cancer, immune disease, and angiogenesis. It has a poor outlook and has been implicated in the development of many types of cancer. We will review the most important applications of this gene in a variety different cancers.

TSPAN8 promotes invasion by NPC cell lines and migration. It is highly expressed in metastatic tumors and is associated with higher histological differentiation and poorer survival. TSPAN8 knockdown, siRNA-mediated silencing, inhibited NPC movement. TSPAN8 overexpression, however, inhibited migration.

TSPAN8 expression in NPC cell lines was the first step to verify its utility as an angiogenesis marker. TSPAN8 gene expression was significantly elevated in NPC cell lines and in their tissues. Transfection of TSPAN8 vector resulted to a significant increase the mRNA and proteins of TSPAN8 in both NPC and S26 cell lines. We then examined the association between TSPAN8 overexpression and patient outcome.

It regulates wound healing

The interaction between epithelial, innate and immune cells is key to the healing of wounds. These cells play an important role in tissue homeostasis, as well as regulating wound healing. These interactions in the skin can have an effect on the migration and regeneration of epithelial cellular cells. In addition, intracellular Ca++ waves stimulate keratinocyte proliferation at the leading edge.

Neutrophils, which are important in promoting the repair of wounds, are involved in the process by consuming large amounts of oxygen and creating a hypoxic microenvironment in the tissue. The chemokine HIF-1a can be stabilized when this happens, leading to enhanced epithelial expressions and intestinal trefoil. These monocytes promote wound healing by recruiting immune cells and promoting restitution of the injured barrier.

TGF-b1 plays an important role in signaling proteins. This molecule regulates wound healing by controlling cell migration and adhesion. It also plays an important role in regulating inflammation and cell proliferation. This growth factor, TGF-1b1, is essential for wound healing. TGF-b1 could be insufficient in the body. TGF-b1 plays an important role in wound healing but it also has other functions.

The MMP-9 enzyme can have a negative effect on healing. The degradation of MMP-9 results in an abnormally recalcitrant wound. The enzyme is highly regulated by diabetic tissues. The treatment that inhibits MMP-9 activity in diabetic animals will speed up wound healing. This enzyme can also promote wound heal by inhibiting MMP-8. So, while MMP-8 plays a positive role in wound repair, MMP-9 inhibits the healing process.