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Product Info Summary
| SKU: | FC03780-PerCP-Cy5.5 |
|---|---|
| Size: | 100 tests, 200 tests |
| Reactive Species: | Cynomolgus monkey, Human, Baboon, Rhesus monkey |
| Host: | Mouse |
| Application: | Flow Cytometry |
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Product info
Product Name
Anti-human CD20 PerCP MS4A1 Monoclonal Antibody PerCP Cy5.5 Conjugated, Flow Validated
SKU/Catalog Number
FC03780-PerCP-Cy5.5
Size
100 tests, 200 tests
Form
Liquid
Description
Boster Bio Anti-human CD20 PerCP MS4A1 Monoclonal Antibody PerCP Cy5.5 Conjugated, Flow Validated (Catalog# FC03780-PerCP-Cy5.5). Tested in Flow Cytometry application(s). This antibody reacts with Human, Rhesus monkey, Cynomolgus monkey, Baboon.
Storage & Handling
Store at 2-8°C. Avoid repeated freeze-thaw cycles.
Cite This Product
Anti-human CD20 PerCP MS4A1 Monoclonal Antibody PerCP Cy5.5 Conjugated, Flow Validated (Boster Biological Technology, Pleasanton CA, USA, Catalog # FC03780-PerCP-Cy5.5)
Host
Mouse
Contents
PBS pH 7.2, 0.2% (w/v) BSA, 0.09% (w/v) sodium azide
Clonality
Monoclonal
Clone Number
Clone: 2H7
Isotype
IgG2b,k
Immunogen
Human tonsillar B cells
*Blocking peptide can be purchased. Costs vary based on immunogen length. Contact us for pricing.
Cross-reactivity
No cross reactivity with other proteins.
Reactive Species
FC03780-PerCP-Cy5.5 is reactive to MS4A1 in Cynomolgus monkey, Human, Baboon, Rhesus monkey
Reconstitution
Observed Molecular Weight
68 kDa
Calculated molecular weight
33077 MW
Background of CD20
The alpha-beta heterodimeric T-cell antigen receptor binds antigen in association with major histocompatibility complex proteins on host cell surfaces. These 2 disulfide-linked glycoproteins, TCRA and TCRB are associated on T-cell surfaces with a complex of proteins called CD3 (formerly T3). Human CD3 consists of at least 4 proteins: gamma, delta, epsilon, and zeta. The CD3-gamma/CD3-delta gene pair is within 300 kb of the CD3-epsilon gene and therefore these genes form a tightly linked cluster on 11q23. The clustering may be significant in terms of their simultaneous activations during T-cell development.
Antibody Validation
Boster validates all antibodies on WB, IHC, ICC, Immunofluorescence, and ELISA with known positive control and negative samples to ensure specificity and high affinity, including thorough antibody incubations.
Application & Images
Applications
FC03780-PerCP-Cy5.5 is guaranteed for Flow Cytometry Boster Guarantee
Assay Dilutions Recommendation
The recommendations below provide a starting point for assay optimization. The actual working concentration varies and should be decided by the user.
User needs to optimize the dilution ratio for this antibody.
Validation Images & Assay Conditions
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Lymphocytes gated human PBMCs stained with mouse anti-human CD20 PerCP-Cyanine5.5 (clone 2H7, red histogram). Blue histogram is for the PBMCs stained with PerCP-Cyanine5.5 conjugated isotype control.
Protein Target Info & Infographic
Gene/Protein Information For MS4A1 (Source: Uniprot.org, NCBI)
Gene Name
MS4A1
Full Name
B-lymphocyte antigen CD20
Weight
33077 MW
Superfamily
MS4A family
Alternative Names
B-lymphocyte antigen CD20;B-lymphocyte surface antigen B1;Bp35;Leukocyte surface antigen Leu-16;Membrane-spanning 4-domains subfamily A member 1;CD20;MS4A1;CD20; MS4A1 B1, Bp35, CD20, CVID5, FMC7, LEU-16, MS4A2, S7 membrane spanning 4-domains A1 B-lymphocyte CD20|B-lymphocyte cell-surface B1|CD20 |CD20 receptor|leukocyte surface Leu-16|membrane-spanning 4-domains, subfamily A, member 1
*If product is indicated to react with multiple species, protein info is based on the gene entry specified above in "Species".For more info on MS4A1, check out the MS4A1 Infographic
We have 30,000+ of these available, one for each gene! Check them out.
In this infographic, you will see the following information for MS4A1: database IDs, superfamily, protein function, synonyms, molecular weight, chromosomal locations, tissues of expression, subcellular locations, post-translational modifications, and related diseases, research areas & pathways. If you want to see more information included, or would like to contribute to it and be acknowledged, please contact [email protected].
Specific Publications For Anti-human CD20 PerCP MS4A1 Monoclonal Antibody PerCP Cy5.5 Conjugated, Flow Validated (FC03780-PerCP-Cy5.5)
Hello CJ!
FC03780-PerCP-Cy5.5 has been cited in 2 publications:
*The publications in this section are manually curated by our staff scientists. They may differ from Bioz's machine gathered results. Both are accurate. If you find a publication citing this product but is missing from this list, please let us know we will issue you a thank-you coupon.
Zinc Prevents Abdominal Aortic Aneurysm Formation by Induction of A20-Mediated Suppression of NF-?B Pathway
Ni C, Chen Y, Zeng M, Pei R, Du Y, Tang L, Wang M, Hu Y, Zhu H, He M, Wei X, Wang S, Ning X, Wang M, Wang J, Ma L, Chen X, Sun Q, Tang H, Wang Y, Wang X. Cell Res. 2015 Jul;25(7):785-800. Doi: 10.1038/Cr.2015.50. Epub 2015 Apr 28. In-Cell Infectio...
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Customer Q&As
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3 Customer Q&As for Anti-human CD20 PerCP MS4A1 Monoclonal Antibody PerCP Cy5.5 Conjugated, Flow Validated
Question
I was wanting to use to test anti-human CD20 PerCP Monoclonal antibody PerCP Cy5.5 Conjugated, Flow Validated FC03780-PerCP-Cy5.5 on human lymph for research purposes, then I may be interested in using anti-human CD20 PerCP Monoclonal antibody PerCP Cy5.5 Conjugated, Flow Validated FC03780-PerCP-Cy5.5 for diagnostic purposes as well. Is the antibody suitable for diagnostic purposes?
B. Moore
Verified customer
Asked: 2019-11-01
Answer
The products we sell, including anti-human CD20 PerCP Monoclonal antibody PerCP Cy5.5 Conjugated, Flow Validated FC03780-PerCP-Cy5.5, are only intended for research use. They would not be suitable for use in diagnostic work. If you have the means to develop a product into diagnostic use, and are interested in collaborating with us and develop our product into an IVD product, please contact us for more discussions.
Boster Scientific Support
Answered: 2019-11-01
Question
I was wanting to use your anti-human CD20 PerCP Monoclonal antibody PerCP Cy5.5 Conjugated, Flow Validated for Flow Cytometry for human lymph on frozen tissues, but I want to know if it has been validated for this particular application. Has this antibody been validated and is this antibody a good choice for human lymph identification?
Verified Customer
Verified customer
Asked: 2019-10-25
Answer
You can see on the product datasheet, FC03780-PerCP-Cy5.5 anti-human CD20 PerCP Monoclonal antibody PerCP Cy5.5 Conjugated, Flow Validated has been validated for Flow Cytometry on human tissues. We have an innovator award program that if you test this antibody and show it works in human lymph in IHC-frozen, you can get your next antibody for free.
Boster Scientific Support
Answered: 2019-10-25
Question
Is this FC03780-PerCP-Cy5.5 anti-human CD20 PerCP Monoclonal antibody PerCP Cy5.5 Conjugated, Flow Validated reactive to the isotypes of MS4A1?
Verified Customer
Verified customer
Asked: 2019-10-15
Answer
The immunogen of FC03780-PerCP-Cy5.5 anti-human CD20 PerCP Monoclonal antibody PerCP Cy5.5 Conjugated, Flow Validated is Human tonsillar B cells. Could you tell me which isotype you are interested in so I can help see if the immunogen is part of this isotype?
Boster Scientific Support
Answered: 2019-10-15
