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Product Info Summary
| SKU: | PA2000 |
|---|---|
| Size: | 100 μg/vial |
| Reactive Species: | Human, Rat |
| Host: | Rabbit |
| Application: | IHC, WB |
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Product info
Product Name
Anti-Tyrosine-protein kinase Tec Tec Antibody Picoband®
SKU/Catalog Number
PA2000
Size
100 μg/vial
Form
Lyophilized
Description
Boster Bio Anti-Tyrosine-protein kinase Tec Tec Antibody catalog # PA2000. Tested in IHC, WB applications. This antibody reacts with Human, Rat. The brand Picoband indicates this is a premium antibody that guarantees superior quality, high affinity, and strong signals with minimal background in Western blot applications. Only our best-performing antibodies are designated as Picoband, ensuring unmatched performance.
Storage & Handling
Store at -20˚C for one year from date of receipt. After reconstitution, at 4˚C for one month. It can also be aliquotted and stored frozen at -20˚C for six months. Avoid repeated freeze-thaw cycles.
Cite This Product
Anti-Tyrosine-protein kinase Tec Tec Antibody Picoband® (Boster Biological Technology, Pleasanton CA, USA, Catalog # PA2000)
Host
Rabbit
Contents
Each vial contains 5mg BSA, 0.9mg NaCl, 0.2mg Na2HPO4, 0.01mg Thimerosal, 0.01mg NaN3.
Clonality
Polyclonal
Isotype
Rabbit IgG
Immunogen
A synthetic peptide corresponding to a sequence at the C-terminus of human Tec, identical to the related rat sequence, and different from the related mouse sequence by one amino acid.
*Blocking peptide can be purchased. Costs vary based on immunogen length. Contact us for pricing.
Cross-reactivity
No cross-reactivity with other proteins
Reactive Species
PA2000 is reactive to TEC in Human, Rat
Reconstitution
Add 0.2ml of distilled water will yield a concentration of 500ug/ml.
Observed Molecular Weight
74 kDa
Calculated molecular weight
73581 MW
Background of TEC
TEC (TEC Protein Tyrosine Kinase), is an enzyme that in humans is encoded by the TEC gene. The protein encoded by this gene belongs to the Tec family of non-receptor protein-tyrosine kinases containing a pleckstrin homology domain. By fluorescence in situ hybridization, Sato et al. (1994) mapped the gene to 4p12, the same location reported for TXK. Mouse Tec is a non-receptor type protein-tyrosine kinase that is highly expressed in many hematopoietic cell lines. Hantschel et al. (2007) identified TEC kinase and BTK kinase as major binders of the tyrosine kinase inhibitor dasatinib, which is used for treatment of BCR/ABL-positive CML.
Antibody Validation
Boster validates all antibodies on WB, IHC, ICC, Immunofluorescence, and ELISA with known positive control and negative samples to ensure specificity and high affinity, including thorough antibody incubations.
Application & Images
Applications
PA2000 is guaranteed for IHC, WB Boster Guarantee
Assay Dilutions Recommendation
The recommendations below provide a starting point for assay optimization. The actual working concentration varies and should be decided by the user.
Western blot, 0.1-0.5μg/ml, Human, Rat
Immunohistochemistry (Paraffin-embedded Section), 0.5-1μg/ml, Human, Rat, By Heat
Positive Control
WB: human HEL whole cell, rat kidney tissue
IHC: Human Intestinal Cancer Tissue, Rat Liver Tissue
Validation Images & Assay Conditions
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Figure 1. Western blot analysis of Tec using anti-Tec antibody (PA2000).
Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel) / 90V (Resolving gel) for 2-3 hours. The sample well of each lane was loaded with 30 ug of sample under reducing conditions.
Lane 1: human HEL whole cell lysates,
Lane 2: rat kidney tissue lysates.
After electrophoresis, proteins were transferred to a nitrocellulose membrane at 150 mA for 50-90 minutes. Blocked the membrane with 5% non-fat milk/TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-Tec antigen affinity purified polyclonal antibody (Catalog # PA2000) at 0.5 μg/mL overnight at 4°C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:5000 for 1.5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit (Catalog # EK1002) with Tanon 5200 system. A specific band was detected for Tec at approximately 74 kDa. The expected band size for Tec is at 74 kDa.
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Figure 2. IHC analysis of Tec using anti-Tec antibody (PA2000).
Tec was detected in a paraffin-embedded section of Human Intestinal Cancer tissue. Heat mediated antigen retrieval was performed in EDTA buffer (pH 8.0, epitope retrieval solution). The tissue section was blocked with 10% goat serum. The tissue section was then incubated with 1 μg/ml rabbit anti-Tec Antibody (PA2000) overnight at 4°C. Peroxidase Conjugated Goat Anti-rabbit IgG was used as secondary antibody and incubated for 30 minutes at 37°C. The tissue section was developed using HRP Conjugated Rabbit IgG Super Vision Assay Kit (Catalog # SV0002) with DAB as the chromogen.
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Figure 3. IHC analysis of Tec using anti-Tec antibody (PA2000).
Tec was detected in a paraffin-embedded section of Rat Liver tissue. Heat mediated antigen retrieval was performed in EDTA buffer (pH 8.0, epitope retrieval solution). The tissue section was blocked with 10% goat serum. The tissue section was then incubated with 1 μg/ml rabbit anti-Tec Antibody (PA2000) overnight at 4°C. Peroxidase Conjugated Goat Anti-rabbit IgG was used as secondary antibody and incubated for 30 minutes at 37°C. The tissue section was developed using HRP Conjugated Rabbit IgG Super Vision Assay Kit (Catalog # SV0002) with DAB as the chromogen.
Protein Target Info & Infographic
Gene/Protein Information For TEC (Source: Uniprot.org, NCBI)
Gene Name
TEC
Full Name
Tyrosine-protein kinase Tec
Weight
73581 MW
Superfamily
protein kinase superfamily
Alternative Names
Tyrosine-protein kinase Tec;2.7.10.2;TEC;PSCTK4; TEC PSCTK4 tec protein tyrosine kinase tyrosine-protein kinase Tec
*If product is indicated to react with multiple species, protein info is based on the gene entry specified above in "Species".For more info on TEC, check out the TEC Infographic
We have 30,000+ of these available, one for each gene! Check them out.
In this infographic, you will see the following information for TEC: database IDs, superfamily, protein function, synonyms, molecular weight, chromosomal locations, tissues of expression, subcellular locations, post-translational modifications, and related diseases, research areas & pathways. If you want to see more information included, or would like to contribute to it and be acknowledged, please contact [email protected].
Specific Publications For Anti-Tyrosine-protein kinase Tec Tec Antibody Picoband® (PA2000)
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Customer Q&As
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3 Customer Q&As for Anti-Tyrosine-protein kinase Tec Tec Antibody Picoband®
Question
Does anti-Tec antibody PA2000 work for IHC with lower esophagus mucosa?
R. Baker
Verified customer
Asked: 2020-02-11
Answer
According to the expression profile of lower esophagus mucosa, TEC is highly expressed in lower esophagus mucosa. So, it is likely that anti-Tec antibody PA2000 will work for IHC with lower esophagus mucosa.
Boster Scientific Support
Answered: 2020-02-11
Question
We are currently using anti-Tec antibody PA2000 for mouse tissue, and we are content with the WB results. The species of reactivity given in the datasheet says human, mouse, rat. Is it likely that the antibody can work on monkey tissues as well?
Verified Customer
Verified customer
Asked: 2019-05-21
Answer
The anti-Tec antibody (PA2000) has not been validated for cross reactivity specifically with monkey tissues, though there is a good chance of cross reactivity. We have an innovator award program that if you test this antibody and show it works in monkey you can get your next antibody for free. Please contact me if I can help you with anything.
Boster Scientific Support
Answered: 2019-05-21
Question
I see that the anti-Tec antibody PA2000 works with IHC, what is the protocol used to produce the result images on the product page?
Verified Customer
Verified customer
Asked: 2018-08-13
Answer
You can find protocols for IHC on the "support/technical resources" section of our navigation menu. If you have any further questions, please send an email to [email protected]
Boster Scientific Support
Answered: 2018-08-13
