TEC Antibodies

Tyrosine-protein kinase Tec (TEC)

Non-receptor tyrosine kinase that leads to signaling from many receptors and participates as a signal transducer in several downstream pathways, such as regulation of the actin cytoskeleton. Plays a redundant part to ITK in regulation of the adaptive immune reaction.

Regulates the development, function and differentiation of traditional T-cells and nonconventional NKT-cells. Required for TCR-dependent IL2 gene induction.

Gene Information

Human
Gene Name:	TEC
Uniprot:	P42680
Entrez:	7006

Family

Belongs to:
protein kinase superfamily

Alternative Names

EC 2.7.10; EC 2.7.10.2; MGC126760; MGC126762; PSCTK4; PSCTK4tyrosine-protein kinase Tec; tec protein tyrosine kinase; Tec

Molecular Weight

Mass (kDA):
73.581 kDA

Genomic Context

Human
Location:	4p12-p11
Sequence:	4; NC_000004.12 (48135783..48269864, complement)

Tissue Specificity

Expressed in a wide range of cells, including hematopoietic cell lines like myeloid, B-, and T-cell lineages.

Subcellular Localizations

Cytoplasm. Cell membrane; Peripheral membrane protein. Cytoplasm, cytoskeleton. Following B-cell or T-cell receptors activation by antigen, translocates to the plasma membrane through its PH domain. Thrombin and integrin engagement induces translocation of TEC to the cytoskeleton during platelet activation. In cardiac myocytes, assumes a diffuse intracellular localization under basal conditions but is recruited to striated structures upon various stimuli, including ATP (By similarity).

Diseases

• Neoplasms
• Tissue Adhesions
• Malignant Neoplasms
• Autoimmune Reaction
• Inflammation
• Kidney Diseases
• Autoimmune Diseases
• Carcinoma
• Injury To Kidney
• Infective Disorder
• Nephritis
• Graft Occlusion, Vascular
• Disease Of Adrenal Medulla

• Tumor Angiogenesis
• Anemia
• Pathologic Neovascularization
• Stenosis
• Thrombosis
• Immunologic Deficiency Syndromes
• Infarction

Interacting Proteins

• GAB1
• MET

Pathways

• Secretion
• Cell Proliferation
• Pathogenesis
• Cell Development
• Angiogenesis
• Cytokine Production
• Cell Differentiation
• Aging
• Regeneration
• Immune Response
• Cell Death
• Cell Adhesion
• Cell Activation

• Localization
• T Cell Differentiation
• T Cell Activation
• Cell Cycle
• Inflammatory Response
• Transport
• Cell Growth

PTMs

• Phosphorylation
• Cleavage
• Acetylation
• Biotinylation
• Nitration

• Glycosylation
• Oxidation
• Methylation
• Dephosphorylation
• Deglycosylation

For details, visit:
bosterbio.com/bosterbio-gene-info-cards/TEC

Best Uses Of The TEC Marker

Boster Bio Anti-von Willebrand Factor / Factor VIII Related-Ag is an endothelial-cell-protective antibody. It is used in a variety of laboratory settings for testing. Its results can be submitted for species-specific or application-specific research and scientists could be eligible for product credits. This antibody is widely available and useful to researchers from all over world.

Boster Bio Anti-von Willebrand Factor (Factor VIII Related-Ag) (Endothelial Marker)

Multimeric glycoproteins, like von Willebrand factor can be found in endothelial cells. It acts as a transporter protein for the coagulation factor VIII, and helps promote platelet adhesion. Some lesions are considered to be endothelial but not all. Antibodies to this particular antigen can help distinguish vascular lesions from endothelial cells.

The antibody is derived from human blood endothelial cells. It binds to human blood cytoplasm. Human endocardium as well as platelets contain more than 25 ULVWF multimeric strings. It also stain fibroblasts, Keratinocytes and Langerhans cells found in the skin.

This product is derived from the cultured HUVECs. The antibody is denatured, and then enriched in ULVWF. The VWF monomer subunit is 250 kDa. The antibody is compatible with the majority of other immunoassays. It is recommended to follow the guidelines carefully before purchasing a test.

Anti-FVIII antibody in vitro is an effective inhibitor of FVIII activity. Anti-FVIII antibody reduces FVIII activity in cell culture supernatants. The inhibition was confirmed through the reduction that occurred in parallel HUVEC lysates. The samples were prepared in T75 flasks and dissolved in 0.5 milliliters of mammalian cells lysis Reagent. The results are the mean plus standard deviations.

To determine the presence of VWF in HUVECs, GMVECs, and fibroblasts were stained with the appropriate antibodies. VWF and FVIII was detected in GMVECs and HUVECs. Additionally, VWF and Factor H could be found in WPBs as well in GMVECs.

Boster Bio Anti-VWDF/F8 similar Ag product is made from human umbilical vein epithelial cells. Umbilical cord blood was cleansed by phosphate buffer before being infused with collagenase solution (0.02 percent in phosphate-buffered sodium). After centrifugation at 250 grams for 10 minutes, the cells were resuspended into Medium 199. The medium contained 10% to 20% heat-inactivated fetal bovine serum, l-glutamine, as well as l-glutamine.

The Boster Bio Anti-VWF/FVIII Ag is an anti-mouse monoclonal antibody. F8-5.5.72 was the clone used. For the mRNA copy count of each sample the normalized gene copy numbers were counted. The GMVEC gene copy number and DDCT were used as positive control. The negative power of the DDCT value was multiplied by 2 to calculate values.

The study showed that anti-VWF/VIII antibodies can be useful in identifying pulmonary vascular disease. Vagina-related infections and kidneys are common complication of chronic renal failure. Boster Bio Anti–VWF/FVIII Related Ag is a tool for diagnosing thrombocytopenia, which is a complication from HUS.

This antibody is anti-VWF and has an extremely high affinity and is highly specific to human VWF. This antibody is a monomer that is soluble, which binds ULVWF/Stx-1 and can penetrate the membranes of endothelial cells. It is used to identify the presence of vascular disease and to treat bleeding issues.

This antibody detects antibodies to VWF/FVIII, by analyzing platelet adhesion and cleavage. It is highly specific for VWF and can be used as a complement medication to treat an existing illness. In vitro studiesshow that this antibody is also sensitive to the detection of endothelial cell.

While it is a highly efficient treatment for VWF/FVIII the drug is not a good predictor of hospitalization and mortality. The cholera toxin which connects to GM1 receptors in endothelial cells are responsible for the drug's cytotoxicity. It is able to bind to GLb3 receptors in endothelial cells and delay ADAMT13 cutting.

It can also detect the levels of vWF in the bloodstream (microRNA-126) that are both indicators of endothelial cells damage. The levels in the serum of these proteins produced by endothelial cells were higher after HII exercise compared to non-exercising controls. These results suggest that levels of vWF in the blood and microRNA-126 are significantly higher during exercise than after HII or MOD exercise.

This antibody recognizes FVIII the protein that is found in endothelial cells. The antibody is specific for FVIII and b-actinwhich are two of the most common endothelial cell markers. It cannot detect the factor H antibody. For this reason, it is not recommended for clinical use.