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Product Info Summary
| SKU: | M03263 |
|---|---|
| Size: | 100 μl/vial |
| Reactive Species: | Human |
| Host: | Rabbit |
| Application: | WB |
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Product info
Product Name
Anti-TEAD1/2/3/4 Rabbit Monoclonal Antibody
View all TEAD1/2/3/4 Antibodies
SKU/Catalog Number
M03263
Size
100 μl/vial
Form
Liquid
Description
Boster Bio Anti-TEAD1/2/3/4 Rabbit Monoclonal Antibody catalog # M03263. Tested in WB application. This antibody reacts with Human.
Storage & Handling
Store at -20°C for one year. For short term storage and frequent use, store at 4°C for up to one month. Avoid repeated freeze-thaw cycles.
Cite This Product
Anti-TEAD1/2/3/4 Rabbit Monoclonal Antibody (Boster Biological Technology, Pleasanton CA, USA, Catalog # M03263)
Host
Rabbit
Contents
Rabbit IgG in phosphate buffered saline, pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol, 0.4-0.5mg/ml BSA.
Clonality
Monoclonal
Clone Number
22T19
Isotype
IgG
Immunogen
A synthesized peptide derived from human TEAD1 + TEAD2 + TEAD3 + TEAD4
*Blocking peptide can be purchased. Costs vary based on immunogen length. Contact us for pricing.
Reactive Species
M03263 is reactive to TEAD1/2/3/4 in Human
Reconstitution
Restore with deionized water (or equivalent) for reconstitution volume of 1.0 mL
Observed Molecular Weight
54 kDa
Calculated molecular weight
54 kDa
Antibody Validation
Boster validates all antibodies on WB, IHC, ICC, Immunofluorescence, and ELISA with known positive control and negative samples to ensure specificity and high affinity, including thorough antibody incubations.
Application & Images
Applications
M03263 is guaranteed for WB Boster Guarantee
Assay Dilutions Recommendation
The recommendations below provide a starting point for assay optimization. The actual working concentration varies and should be decided by the user.
WB 1:500-1:1000
Positive Control
WB: human Hela whole cell, human SiHa whole cell, human A549 whole cell
Validation Images & Assay Conditions
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Figure 1. Western blot analysis of TEAD1/2/3/4 using anti-TEAD1/2/3/4 antibody (M03263).
Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel) / 90V (Resolving gel) for 2-3 hours. The sample well of each lane was loaded with 30 ug of sample under reducing conditions.
Lane 1: human Hela whole cell lysates,
Lane 2: human SiHa whole cell lysates,
Lane 3: human A549 whole cell lysates.
After electrophoresis, proteins were transferred to a nitrocellulose membrane at 150 mA for 50-90 minutes. Blocked the membrane with 5% non-fat milk/TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-TEAD1/2/3/4 antigen affinity purified monoclonal antibody (Catalog # M03263) at 1:500 overnight at 4°C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:500 for 1.5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit (Catalog # EK1002) with Tanon 5200 system. A specific band was detected for TEAD1/2/3/4 at approximately 54 kDa. The expected band size for TEAD1/2/3/4 is at 54 kDa.
Protein Target Info & Infographic
Gene/Protein Information For TEAD1/2/3/4 (Source: Uniprot.org, NCBI)
Gene Name
TEAD1/2/3/4
Full Name
Weight
54 kDa
Alternative Names
Claudin-5;Transmembrane protein deleted in VCFS;TMDVCF;CLDN5;AWAL, TMVCF;
*If product is indicated to react with multiple species, protein info is based on the gene entry specified above in "Species".For more info on TEAD1/2/3/4, check out the TEAD1/2/3/4 Infographic
We have 30,000+ of these available, one for each gene! Check them out.
In this infographic, you will see the following information for TEAD1/2/3/4: database IDs, superfamily, protein function, synonyms, molecular weight, chromosomal locations, tissues of expression, subcellular locations, post-translational modifications, and related diseases, research areas & pathways. If you want to see more information included, or would like to contribute to it and be acknowledged, please contact [email protected].
Specific Publications For Anti-TEAD1/2/3/4 Rabbit Monoclonal Antibody (M03263)
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