Anti-TDP43 TARDBP Antibody

Product Name

Anti-TDP43 TARDBP Antibody

View all TDP-43/TARDBP Antibodies

SKU/Catalog Number

A01001

Size

0.1 mg

Form

Liquid

Description

Boster Bio Anti-TDP43 TARDBP Antibody (Catalog # A01001). Tested in ELISA, WB, ICC, IF applications. This antibody reacts with Human, Mouse, Rat.

Storage & Handling

TDP43 antibody can be stored at 4°C for three months and -20°C, stable for up to one year. Avoid repeated freeze-thaw cycles. Antibodies should not be exposed to prolonged high temperatures.

Cite This Product

Anti-TDP43 TARDBP Antibody (Boster Biological Technology, Pleasanton CA, USA, Catalog # A01001)

Host

Rabbit

Contents

TDP43 Antibody is supplied in PBS containing 0.02% sodium azide.

Clonality

Polyclonal

Isotype

IgG

Immunogen

TDP43 antibody was raised against a 15 amino acid synthetic peptide from near the amino terminus of human TDP43. The immunogen is located within amino acids 60 - 110 of TDP43.

*Blocking peptide can be purchased. Costs vary based on immunogen length. Contact us for pricing.

Cross-reactivity

At least two isoforms are known to exist for this protein; this TDP43 antibody will recognize both isoforms.

Reactive Species

A01001 is reactive to TARDBP in Human, Mouse, Rat

Applications

A01001 is guaranteed for ELISA, IF, ICC, WB Boster Guarantee

Observed Molecular Weight

68 kDa

Calculated molecular weight

44740 MW

Background of TDP-43/TARDBP

TDP43 was initially identified as a novel cellular protein that bound to HIV-1 virus TAR DNA sequence motifs and acts a transcriptional repressor to the HIV-1 LTR. Later experiments revealed that TDP43 also regulates the splicing of exon 9 of the cystic fibrosis transmembrane conductance regular (CFTR), most likely through the association with the UG repeats at the 3'splice site. A hyperphosphorylated, ubiquitinated, and cleaved form of TDP43 known as pathologic TDP43 is the major disease protein in ubiquitin-positive, tau-, and alpha-synuclein-negative frontotemporal dementia (FLTD-U). TDP43 is not related to TRBP1, and RNA binding protein that binds HIV-1 TAR RNA sequences.

Antibody Validation

Boster validates all antibodies on WB, IHC, ICC, Immunofluorescence, and ELISA with known positive control and negative samples to ensure specificity and high affinity, including thorough antibody incubations.

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Assay Dilutions Recommendation

The recommendations below provide a starting point for assay optimization. The actual working concentration varies and should be decided by the user.

TDP43 antibody can be used for detection of TDP43 by Western blot at 0.5 - 2 μg/mL. Antibody can also be used for immunocytochemistry starting at 5 μg/mL. For immunofluorescence start at 20 μg/mL.
Antibody validated: Western Blot in human samples; Immunocytochemistry in human samples and Immunofluorescence in human samples. All other applications and species not yet tested. Optimal dilutions for each application should be determined by the researcher.

Validation Images & Assay Conditions

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Western blot analysis of TDP43 in HeLa cell lysate with TDP43 antibody at (A) 0.5

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Immunocytochemistry of TDP43 in HeLa cells with TDP43 antibody at 5 μg/mL.

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Immunofluorescence of TDP43 in Hela cells with TDP43 antibody at 20 μg/mL.

Gene/Protein Information For TARDBP (Source: Uniprot.org, NCBI)

Alternative Names

ALS10; ALS10TAR DNA-binding protein-43; TAR DNA binding protein; TARDBP; TDP43; TDP-43; TDP-43TAR DNA-binding protein 43 TARDBP ALS10, TDP-43 TAR DNA binding protein TAR DNA-binding protein 43|TAR DNA-binding protein-43

For more info on TARDBP, check out the TARDBP Infographic

We have 30,000+ of these available, one for each gene! Check them out.

In this infographic, you will see the following information for TARDBP: database IDs, superfamily, protein function, synonyms, molecular weight, chromosomal locations, tissues of expression, subcellular locations, post-translational modifications, and related diseases, research areas & pathways. If you want to see more information included, or would like to contribute to it and be acknowledged, please contact [email protected].

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