TARDBP Antibodies

TAR DNA-binding protein 43 (TARDBP)

DNA and RNA-binding protein that regulates transcription and splicing. Involved in the regulation of CFTR splicing.

It promotes CFTR exon 9 skipping by binding to the UG repeated motifs in the polymorphic region near the 3'-splice site of the exon. The resulting aberrant splicing is associated with pathological features typical of cystic fibrosis.

Gene Information

Human
Gene Name:	TARDBP
Uniprot:	Q13148
Entrez:	23435

Family

Belongs to:
No superfamily

Alternative Names

ALS10; ALS10TAR DNA-binding protein-43; TAR DNA binding protein; TARDBP; TDP43; TDP-43; TDP-43TAR DNA-binding protein 43

Molecular Weight

Mass (kDA):
44.74 kDA

Genomic Context

Human
Location:	1p36.22
Sequence:	1; NC_000001.11 (11012654..11030528)

Tissue Specificity

Ubiquitously expressed. In particular, expression is high in pancreas, placenta, lung, genital tract and spleen.

Subcellular Localizations

Nucleus. Cytoplasm. Cytoplasm, Stress granule. Continuously travels in and out of the nucleus (PubMed:18957508). Localizes to stress granules in response to oxidative stress (PubMed:19765185).

Diseases

• Amyotrophic Lateral Sclerosis
• Sclerosis
• Primary Lateral Sclerosis
• Frontotemporal Lobar Degeneration
• Feline Urological Syndrome
• Dementia
• Neurodegenerative Disorders
• Nerve Degeneration
• Frontotemporal Dementia
• Motor Neuron Disease
• Tdp-43 Proteinopathies
• Sarcoma
• Neuropathology Disease

• Impaired Cognition
• Secondary Parkinson Disease
• Alzheimer's Disease
• Weakness
• Parkinson Disease
• Nervousness
• Liposarcoma

Interacting Proteins

• ATXN2
• IRAK2
• PPP1R15A

Pathways

• Pathogenesis
• Rna Processing
• Localization
• Transport
• Mrna Splicing
• Translation
• Cognition
• Rna Splicing
• Locomotion
• Protein Folding
• Cell Adhesion
• Aging
• Stress Granule Assembly

• Embryo Development
• Mitochondrial Fusion
• Cell Division
• Swimming
• Rna Transport
• Cytoplasmic Transport
• Cell Proliferation

PTMs

• Ubiquitination

• Phosphorylation

• Cleavage

Research Areas

• Cell Cycle and Replication
• Chromatin Research
• DNA replication, Transcription, Translation and Splicing
• Epigenetics
• Immunology

• Mitochondrial Fusion Proteins
• Neurodegeneration
• Neuronal Cell Markers
• Neuroscience
• Transcription Factors and Regulators

• Virology, Bacteria and Parasites

For details, visit:
bosterbio.com/bosterbio-gene-info-cards/TARDBP

Best Uses of the Boster Bio Anti-TAR DNA-Binding Protein 43 TARDBP Marker

This article will explain the advantages and disadvantages Boster Bio’s AntiTAR DNA-binding proteins 43 TARDBP markers. We will also discuss safety and cost. As always, we hope that this article will help you decide if it's right for you. And don't forget to check out our other articles for more information! Don't forget about our newsletter!

Boster Bio: Anti-TAR DNA-binding protein 43 TARDBP Marker

The BosterBio Anti-TAR DNA binding protein 43 (TARDBP), Marker, was originally created by a group with an interest in the TAR DNA regions of HIV. The protein binds with the TAR and blocks the TAT protein from binding to mRNA. The protein was cloned a second time by a group studying the CFTR intron region. It is composed a polymorphic (TG),mT,n repeated sequences. These sequences are responsible to skipping exon 9 in CFTR's gene.

TARDBP is a transactive response DNA binding protein of 43 kDa. It can be found in most tissues' nucleus. TDP43 plays a key role in many aspects of protein production. It binds with DNA to control transcription. This is the first step in the production of protein from a genetic material. It also binds with RNA, which acts as the genetic blueprints of protein production.

TDP-43 immunolocalization was performed at all stages of the mouse seminiferous epithelium cycle. The gene was not detected at all in undifferentiated embryogonia. However, its presence was detected at Stage IV in differentiated intermediate embryogonia and at Stage VI in type B-spermatocytes. At Stages IX-XI, TDP-43 levels decreased while those of pachytene spermatocytes increased.

Both monomers and multimers of TDP43 were detected by the GP#2 or GP#3 antibody. Both antibodies recognized TDP43's carboxyl terminus. Higher MW bands were expected from these antibodies. It was interesting, however, to see that both antibodies could detect TDP-43 in its full length. The protein also strongly reacted towards DGly. The bands of GP#2 antisera were stronger that those of Proteintech TDP43 monomer and Abcam.

Clinical applications

TARDBP has been implicated as a cause of ALS, a progressive loss of motor neurons in the upper and lower motors. Although only 4% of cases are familial, a mutation in this gene is associated with about half of all ALS cases. Approximately 90% of ALS patients have some form of TDP-43 pathology. This gene marker could aid physicians in diagnosing the cause of the disease and in guiding clinical management.

The TARDBP gene encodes the protein TDP-43, which belongs to the family of heterogeneous nuclear ribonucleoproteins (hnRNPs). This protein is normally found in the nucleus of cells in normal conditions, but it can also accumulate within the cytoplasm and be implicated as a cause of many diseases. During disease, this protein undergoes cleavage, hyperphosphorylation, and ubiquitination, which can lead to pathological inclusions.

A wider range of disorders have been found to be associated with mutations in TARDBP than previously thought. 2076G-A mutations, for example, are associated with a twofold rise in TARDBP expression. These results suggest that TARDBP could be involved with the pathology of a wider array of disorders than previously thought. Therefore, the TARDBP gene has a wide range of potential clinical applications.

TARDBP gene is a heterozygous transposition in exon 6 that results in an ala382-to-thr substitution in the C-terminal region of TDP43. This mutation has been detected in five patients in two generations, compared with seven47 white subjects and 380 Chinese control subjects. These patients had an early onset of the disease and died within one to two year of being diagnosed. Postmortem examination revealed a variety of clinical changes and TDP43 positive inclusions in brain areas, upper and lower motor neurons, and other tissues.

Cost

TARDBP encodes a protein that has two RNA recognition motif. This protein participates in many processes and shuttles from the cytoplasm to the nucleus. A glycine rich domain is also present in the protein, which is involved with many types of protein–protein interactions. The TARDBP gene is expressed during aggregation. Its costs are similar to that of other proteins.

Safety

TDP-43 is encoded by the TARDBP gene. This protein is found in the cell nucleus in almost all tissues and is involved in many aspects of protein production. TDP-43 binds to DNA and regulates transcription, the first step in protein production from a gene. It also binds with messenger RNA which is responsible for the genetic blueprints that allow for the production of proteins. TARDBP genes are highly conserved in humans. They are expressed throughout the body.

The TARDBP gene is a promising candidate for clinical research and has many clinical uses. The TARDBP gene is involved in several human diseases including ALS and FTD. The marker is used in research to monitor the progression of neurodegenerative diseases, such as Alzheimer's. It has been a vital biomarker for the diagnosis, management, and monitoring of ALS in recent years. It has also been used to develop drug treatments for a number of other diseases.