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Product Info Summary
| SKU: | A01804-1 |
|---|---|
| Size: | 100 μg/vial |
| Reactive Species: | Human, Mouse, Rat |
| Host: | Rabbit |
| Application: | ELISA, IF, ICC, WB |
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Product info
Product Name
Anti-TDP2 Antibody Picoband®
View all ETS1 associated protein II Antibodies
SKU/Catalog Number
A01804-1
Size
100 μg/vial
Form
Lyophilized
Description
Boster Bio Anti-TDP2 Antibody Picoband® catalog # A01804-1. Tested in ELISA, IF, ICC, WB applications. This antibody reacts with Human, Mouse, Rat. The brand Picoband indicates this is a premium antibody that guarantees superior quality, high affinity, and strong signals with minimal background in Western blot applications. Only our best-performing antibodies are designated as Picoband, ensuring unmatched performance.
Storage & Handling
At -20°C for one year from date of receipt. After reconstitution, at 4°C for one month. It can also be aliquotted and stored frozen at -20°C for six months. Avoid repeated freezing and thawing.
Cite This Product
Anti-TDP2 Antibody Picoband® (Boster Biological Technology, Pleasanton CA, USA, Catalog # A01804-1)
Host
Rabbit
Contents
Each vial contains 4 mg Trehalose, 0.9 mg NaCl, 0.2 mg Na2HPO4.
Clonality
Polyclonal
Isotype
Rabbit IgG
Immunogen
E.coli-derived human TDP2 recombinant protein (Position: R25-R332).
*Blocking peptide can be purchased. Costs vary based on immunogen length. Contact us for pricing.
Cross-reactivity
No cross-reactivity with other proteins.
Reactive Species
A01804-1 is reactive to TDP2 in Human, Mouse, Rat
Reconstitution
Adding 0.2 ml of distilled water will yield a concentration of 500 µg/ml.
Observed Molecular Weight
49 kDa
Calculated molecular weight
40.93kDa
Background of ETS1 associated protein II
This gene encodes a member of a superfamily of divalent cation-dependent phosphodiesterases. The encoded protein associates with CD40, tumor necrosis factor (TNF) receptor-75 and TNF receptor associated factors (TRAFs), and inhibits nuclear factor-kappa-B activation. This protein has sequence and structural similarities with APE1 endonuclease, which is involved in both DNA repair and the activation of transcription factors.
Antibody Validation
Boster validates all antibodies on WB, IHC, ICC, Immunofluorescence, and ELISA with known positive control and negative samples to ensure specificity and high affinity, including thorough antibody incubations.
Application & Images
Applications
A01804-1 is guaranteed for ELISA, IF, ICC, WB Boster Guarantee
Assay Dilutions Recommendation
The recommendations below provide a starting point for assay optimization. The actual working concentration varies and should be decided by the user.
Western blot, 0.25-0.5 μg/ml, Human, Mouse, Rat
Immunocytochemistry/Immunofluorescence, 5 μg/ml, Human
ELISA, 0.1-0.5 μg/ml, -
Positive Control
WB: human HepG2 whole cell, human RT4 whole cell, rat brain tissue, mouse brain tissue
ICC/IF: A549 cell
Validation Images & Assay Conditions
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Figure 1. Western blot analysis of TDP2 using anti-TDP2 antibody (A01804-1).
Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel) / 90V (Resolving gel) for 2-3 hours. The sample well of each lane was loaded with 30 ug of sample under reducing conditions.
Lane 1: human HepG2 whole cell lysates,
Lane 2: human RT4 whole cell lysates,
Lane 3: rat brain tissue lysates,
Lane 4: mouse brain tissue lysates.
After electrophoresis, proteins were transferred to a nitrocellulose membrane at 150 mA for 50-90 minutes. Blocked the membrane with 5% non-fat milk/TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-TDP2 antigen affinity purified polyclonal antibody (Catalog # A01804-1) at 0.5 μg/mL overnight at 4°C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:5000 for 1.5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit (Catalog # EK1002) with Tanon 5200 system. A specific band was detected for TDP2 at approximately 49 kDa. The expected band size for TDP2 is at 41 kDa.
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Figure 2. IF analysis of TDP2 using anti-TDP2 antibody (A01804-1).
TDP2 was detected in an immunocytochemical section of A549 cells. Enzyme antigen retrieval was performed using IHC enzyme antigen retrieval reagent (AR0022) for 15 mins. The cells were blocked with 10% goat serum. And then incubated with 5 μg/mL rabbit anti-TDP2 Antibody (A01804-1) overnight at 4°C. DyLight®488 Conjugated Goat Anti-Rabbit IgG (BA1127) was used as secondary antibody at 1:100 dilution and incubated for 30 minutes at 37°C. The section was counterstained with DAPI. Visualize using a fluorescence microscope and filter sets appropriate for the label used.
Protein Target Info & Infographic
Gene/Protein Information For TDP2 (Source: Uniprot.org, NCBI)
Gene Name
TDP2
Full Name
Tyrosyl-DNA phosphodiesterase 2
Weight
40.93kDa
Superfamily
CCR4/nocturin family
Alternative Names
Serine protease HTRA1; High-temperature requirement A serine peptidase 1; L56; Serine protease 11; HTRA1; HTRA; PRSS11 TDP2 AD022, EAP2, EAPII, TTRAP, dJ30M3.3, hTDP2 tyrosyl-DNA phosphodiesterase 2 tyrosyl-DNA phosphodiesterase 2|5-Tyr-DNA phosphodiesterase|5-tyrosyl-DNA phosphodiesterase|ETS1-associated protein 2|ETS1-associated protein II|TRAF and TNF receptor-associated protein|VPg unlinkase|epididymis secretory sperm binding protein|tyr-DNA phosphodiesterase 2|tyrosyl-RNA phosphodiesterase
*If product is indicated to react with multiple species, protein info is based on the gene entry specified above in "Species".For more info on TDP2, check out the TDP2 Infographic
We have 30,000+ of these available, one for each gene! Check them out.
In this infographic, you will see the following information for TDP2: database IDs, superfamily, protein function, synonyms, molecular weight, chromosomal locations, tissues of expression, subcellular locations, post-translational modifications, and related diseases, research areas & pathways. If you want to see more information included, or would like to contribute to it and be acknowledged, please contact [email protected].
Specific Publications For Anti-TDP2 Antibody Picoband® (A01804-1)
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