Product Info Summary
SKU: | M05524-2 |
---|---|
Size: | 100 μl/vial |
Reactive Species: | Human, Mouse, Rat |
Host: | Rabbit |
Application: | Flow Cytometry, IP, IF, IHC, ICC, WB |
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Product info
Product Name
Anti-TCP1 beta Rabbit Monoclonal Antibody
SKU/Catalog Number
M05524-2
Size
100 μl/vial
Form
Liquid
Description
Boster Bio Anti-TCP1 beta Rabbit Monoclonal Antibody catalog # M05524-2. Tested in WB, IHC, ICC/IF, IP, Flow Cytometry applications. This antibody reacts with Human, Mouse, Rat.
Storage & Handling
Store at -20°C for one year. For short term storage and frequent use, store at 4°C for up to one month. Avoid repeated freeze-thaw cycles.
Cite This Product
Anti-TCP1 beta Rabbit Monoclonal Antibody (Boster Biological Technology, Pleasanton CA, USA, Catalog # M05524-2)
Host
Rabbit
Contents
Rabbit IgG in phosphate buffered saline, pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol, 0.4-0.5mg/ml BSA.
Clonality
Monoclonal
Clone Number
22C16
Isotype
IgG
Immunogen
A synthesized peptide derived from human TCP1 beta
*Blocking peptide can be purchased. Costs vary based on immunogen length. Contact us for pricing.
Reactive Species
M05524-2 is reactive to CCT2 in Human, Mouse, Rat
Applications
M05524-2 is guaranteed for Flow Cytometry, IP, IF, IHC, ICC, WB Boster Guarantee
Observed Molecular Weight
57 kDa
Calculated molecular weight
58927 MW
Antibody Validation
Boster validates all antibodies on WB, IHC, ICC, Immunofluorescence, and ELISA with known positive control and negative samples to ensure specificity and high affinity, including thorough antibody incubations.
Assay dilution & Images
Reconstitution
Restore with deionized water (or equivalent) for reconstitution volume of 1.0 mL
Assay Dilutions Recommendation
The recommendations below provide a starting point for assay optimization. The actual working concentration varies and should be decided by the user.
WB 1:1000-1:5000
IHC 1:50-1:200
ICC/IF 1:50-1:200
IP 1:50
FC 1:50
Validation Images & Assay Conditions
Click image to see more details
Figure 1. Western blot analysis of TCP1 Beta using anti-TCP1 Beta antibody (M05524-2).
Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel) / 90V (Resolving gel) for 2-3 hours. The sample well of each lane was loaded with 30 ug of sample under reducing conditions.
Lane 1: human Hela whole cell lysates,
Lane 2: human Raji whole cell lysates,
Lane 3: human T-47D whole cell lysates,
Lane 4: human SW620 whole cell lysates,
Lane 5: rat brain tissue lysates,
Lane 6: rat PC-12 whole cell lysates,
Lane 7: mouse brain tissue lysates,
Lane 8: mouse RAW264.7 whole cell lysates.
After electrophoresis, proteins were transferred to a nitrocellulose membrane at 150 mA for 50-90 minutes. Blocked the membrane with 5% non-fat milk/TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-TCP1 Beta antigen affinity purified monoclonal antibody (Catalog # M05524-2) at 1:500 overnight at 4°C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:1000 for 1.5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit (Catalog # EK1002) with Tanon 5200 system. A specific band was detected for TCP1 Beta at approximately 57 kDa. The expected band size for TCP1 Beta is at 57 kDa.
Protein Target Info & Infographic
Gene/Protein Information For CCT2 (Source: Uniprot.org, NCBI)
Gene Name
CCT2
Full Name
T-complex protein 1 subunit beta
Weight
58927 MW
Superfamily
TCP-1 chaperonin family
Alternative Names
Cctb; CCT-beta; CCTBPRO1633,99D8.1; chaperonin containing t-complex polypeptide 1, beta subunit; chaperonin containing t-complex polypeptide 1, subunit 2; chaperonin containing TCP1, subunit 2 (beta); MGC142074; MGC142076; T-complex protein 1 subunit beta; T-complex protein 1, beta subunit; TCP-1-beta CCT2 99D8.1, CCT-beta, CCTB, HEL-S-100n, PRO1633, TCP-1-beta chaperonin containing TCP1 subunit 2 T-complex protein 1 subunit beta|T-complex protein 1, beta subunit|chaperonin containing TCP1, subunit 2 (beta)|chaperonin containing t-complex polypeptide 1, beta subunit|chaperonin containing t-complex polypeptide 1, subunit 2|epididymis secretory sperm binding protein Li 100n
*If product is indicated to react with multiple species, protein info is based on the gene entry specified above in "Species".For more info on CCT2, check out the CCT2 Infographic
We have 30,000+ of these available, one for each gene! Check them out.
In this infographic, you will see the following information for CCT2: database IDs, superfamily, protein function, synonyms, molecular weight, chromosomal locations, tissues of expression, subcellular locations, post-translational modifications, and related diseases, research areas & pathways. If you want to see more information included, or would like to contribute to it and be acknowledged, please contact [email protected].
Specific Publications For Anti-TCP1 beta Rabbit Monoclonal Antibody (M05524-2)
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