Product Info Summary
SKU: | A07898 |
---|---|
Size: | 100 µl |
Reactive Species: | Human |
Host: | Rabbit |
Application: | WB |
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Product info
Product Name
Anti-Centrosomal protein of 57 kDa CEP57 Antibody
SKU/Catalog Number
A07898
Size
100 µl
Form
Liquid
Description
Boster Bio Anti-Centrosomal protein of 57 kDa CEP57 Antibody (Catalog # A07898). Tested in WB applications. This antibody reacts with Human.
Storage & Handling
Store at -20°C for one year. For short term storage and frequent use, store at 4°C for up to one month. Avoid repeated freeze-thaw cycles.
Cite This Product
Anti-Centrosomal protein of 57 kDa CEP57 Antibody (Boster Biological Technology, Pleasanton CA, USA, Catalog # A07898)
Host
Rabbit
Contents
Rabbit IgG in phosphate buffered saline (without Mg2+ and Ca2+), pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol.
Clonality
Polyclonal
Isotype
IgG
Immunogen
Synthesized peptide derived from internal of human CEP57.
*Blocking peptide can be purchased. Costs vary based on immunogen length. Contact us for pricing.
Reactive Species
A07898 is reactive to CEP57 in Human
Reconstitution
Calculated molecular weight
57089 MW
Background of CEP57
Centrosomal protein which may be required for microtubule attachment to centrosomes. May act by forming ring-like structures around microtubules. Mediates nuclear translocation and mitogenic activity of the internalized growth factor FGF2, but that of FGF1.
Bossard C., Nat. Cell Biol. 5:433-439(2003).
Nagase T., DNA Res. 2:37-43(1995).
Ota T., Nat. Genet. 36:40-45(2004)
Antibody Validation
Boster validates all antibodies on WB, IHC, ICC, Immunofluorescence, and ELISA with known positive control and negative samples to ensure specificity and high affinity, including thorough antibody incubations.
Application & Images
Applications
A07898 is guaranteed for WB Boster Guarantee
Assay Dilutions Recommendation
The recommendations below provide a starting point for assay optimization. The actual working concentration varies and should be decided by the user.
Western blotting: 1:500~1:3000
Validation Images & Assay Conditions
Click image to see more details
Western blot analysis of extracts from LOVO cells and COLO cells, using MRPS7 antibody A07898.
Protein Target Info & Infographic
Gene/Protein Information For CEP57 (Source: Uniprot.org, NCBI)
Gene Name
CEP57
Full Name
Centrosomal protein of 57 kDa
Weight
57089 MW
Superfamily
translokin family
Alternative Names
Centrosomal protein of 57 kDa;Cep57;FGF2-interacting protein;Testis-specific protein 57;Translokin;CEP57;KIAA0092, TSP57; CEP57 MVA2, PIG8, TSP57 centrosomal protein 57 centrosomal protein of 57 kDa|FGF2-interacting protein|centrosomal protein 57kDa|proliferation-inducing protein 8|testis-specific protein 57|translokin
*If product is indicated to react with multiple species, protein info is based on the gene entry specified above in "Species".For more info on CEP57, check out the CEP57 Infographic
We have 30,000+ of these available, one for each gene! Check them out.
In this infographic, you will see the following information for CEP57: database IDs, superfamily, protein function, synonyms, molecular weight, chromosomal locations, tissues of expression, subcellular locations, post-translational modifications, and related diseases, research areas & pathways. If you want to see more information included, or would like to contribute to it and be acknowledged, please contact [email protected].
Specific Publications For Anti-Centrosomal protein of 57 kDa CEP57 Antibody (A07898)
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No publications found for A07898
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Customer Q&As
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6 Customer Q&As for Anti-Centrosomal protein of 57 kDa CEP57 Antibody
Question
Thanks for helping with my inquiry over the phone. Here are the WB image, lot number and protocol we used for kidney using anti-CEP57 antibody A07898. Let me know if you need anything else.
Verified Customer
Verified customer
Asked: 2020-03-23
Answer
Thank you for the data. You have provided everything we needed. Our lab team are working to resolve your inquiry as quickly as possible, and we appreciate your patience and understanding! Please let me know if there is anything you need in the meantime.
Boster Scientific Support
Answered: 2020-03-23
Question
We are currently using anti-CEP57 antibody A07898 for human tissue, and we are happy with the WB results. The species of reactivity given in the datasheet says human. Is it true that the antibody can work on monkey tissues as well?
Verified Customer
Verified customer
Asked: 2019-12-25
Answer
The anti-CEP57 antibody (A07898) has not been validated for cross reactivity specifically with monkey tissues, but there is a good chance of cross reactivity. We have an innovator award program that if you test this antibody and show it works in monkey you can get your next antibody for free. Please contact me if I can help you with anything.
Boster Scientific Support
Answered: 2019-12-25
Question
I see that the anti-CEP57 antibody A07898 works with WB, what is the protocol used to produce the result images on the product page?
Verified Customer
Verified customer
Asked: 2019-08-14
Answer
You can find protocols for WB on the "support/technical resources" section of our navigation menu. If you have any further questions, please send an email to [email protected]
Boster Scientific Support
Answered: 2019-08-14
Question
Is this A07898 anti-CEP57 antibody reactive to the isotypes of CEP57?
Verified Customer
Verified customer
Asked: 2019-05-28
Answer
The immunogen of A07898 anti-CEP57 antibody is Synthesized peptide derived from internal of human CEP57. Could you tell me which isotype you are interested in so I can help see if the immunogen is part of this isotype?
Boster Scientific Support
Answered: 2019-05-28
Question
Would anti-CEP57 antibody A07898 work for WB with kidney?
Verified Customer
Verified customer
Asked: 2019-05-22
Answer
According to the expression profile of kidney, CEP57 is highly expressed in kidney. So, it is likely that anti-CEP57 antibody A07898 will work for WB with kidney.
Boster Scientific Support
Answered: 2019-05-22
Question
Can you help my question with product A07898, anti-CEP57 antibody. I was wondering if it would be possible to conjugate this antibody with biotin. I would need it to be without BSA or sodium azide. I am planning on using a buffer exchange of sodium azide with PBS only. Would there be problems for me to conjugate the antibody and store it in -20 degrees in small aliquots?
Verified Customer
Verified customer
Asked: 2019-02-18
Answer
We suggest not storing this antibody with PBS buffer only in -20 degrees. If you want to store it in -20 degrees it is best to add some cryoprotectant like glycerol. If you want carrier free A07898 anti-CEP57 antibody, we can provide it to you in a special formula with trehalose and/or glycerol. These molecules will not interfere with conjugation chemistry and provide a good level of protection for the antibody from degradation. Please be sure to specify this in your purchase order.
Boster Scientific Support
Answered: 2019-02-18