Product Info Summary
SKU: | A05319 |
---|---|
Size: | 0.1 mg |
Reactive Species: | Human, Mouse, Rat |
Host: | Rabbit |
Application: | ELISA, IF, IHC-P, WB |
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Product info
Product Name
Anti-TAF9 Antibody
SKU/Catalog Number
A05319
Size
0.1 mg
Form
Liquid
Description
Boster Bio Anti-TAF9 Antibody (Catalog # A05319). Tested in ELISA, WB, IHC-P, IF applications. This antibody reacts with Human, Mouse, Rat.
Storage & Handling
TAF9 antibody can be stored at 4°C for three months and -20°C, stable for up to one year.
Cite This Product
Anti-TAF9 Antibody (Boster Biological Technology, Pleasanton CA, USA, Catalog # A05319)
Host
Rabbit
Contents
TAF9 Antibody is supplied in PBS containing 0.02% sodium azide.
Clonality
Polyclonal
Isotype
IgG
Immunogen
TAF9 antibody was raised against a 17 amino acid peptide near the center of human TAF9. The immunogen is located within amino acids 110 - 160 of TAF9.
*Blocking peptide can be purchased. Costs vary based on immunogen length. Contact us for pricing.
Cross-reactivity
TAF9 antibody is human, mouse and rat reactive. Multiple isoforms of TAF9 are known to exist. TAF9 is predicted to not cross-react with other TAF family members.
Reactive Species
A05319 is reactive to TAF9 in Human, Mouse, Rat
Reconstitution
Observed Molecular Weight
68 kDa
Calculated molecular weight
28974 MW
Background of TAF9
Initiation of transcription by RNA polymerase II is coordinated by the transcription factor IID (TFIID), which binds to the core promoter to position the polymerase properly, serves as the scaffold for assembly of the remainder of the transcription complex, and acts as a channel for regulatory signals. TFIID is composed of the TATA-binding protein (TBP) and the TBP-associated factors (TAFs). TAFs may participate in basal transcription, serve as coactivators, function in promoter recognition or modify general transcription factors (GTFs) to facilitate complex assembly and transcription initiation (1). TAF9 is one of the smaller subunits of TFIID that binds to the basal transcription factor GTF2B as well as to several transcriptional activators such as p53 and VP16 (2).
Antibody Validation
Boster validates all antibodies on WB, IHC, ICC, Immunofluorescence, and ELISA with known positive control and negative samples to ensure specificity and high affinity, including thorough antibody incubations.
Application & Images
Applications
A05319 is guaranteed for ELISA, IF, IHC-P, WB Boster Guarantee
Assay Dilutions Recommendation
The recommendations below provide a starting point for assay optimization. The actual working concentration varies and should be decided by the user.
TAF9 antibody can be used for detection of TAF9 by Western blot at 1 - 2 μg/ml. Antibody can also be used for Immunohistochemistry at 5 μg/mL. For Immunoflorescence start at 20 μg/mL.
Antibody validated: Western Blot in human samples; Immunohistochemistry in mouse samples and Immunofluorescence in mouse samples. All other applications and species not yet tested. Optimal dilutions for each application should be determined by the researcher.
Validation Images & Assay Conditions
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Western blot analysis of TAF9 in HepG2 cell lysate with TAF9 antibody at (A) 1 and (B) 2μg/ml.
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Immunohistochemistry of TAF9 in mouse liver tissue with TAF9 antibody at 5 μg/mL.
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Immunofluorescence of TAF9 in mouse liver tissue with TAF9 antibody at 20 μg/mL.
Protein Target Info & Infographic
Gene/Protein Information For TAF9 (Source: Uniprot.org, NCBI)
Gene Name
TAF9
Full Name
Transcription initiation factor TFIID subunit 9
Weight
28974 MW
Superfamily
TAF9 family
Alternative Names
TAF2G, TAFII31, TAFII32, MGC:5067, TAFII-31, TAFII-32, TAFIID32, STAF31/32, TAF2G, Transcription initiation factor TFIID subunit 9, RNA polymerase II TBP-associated factor subunit G TAF9 MGC:5067, STAF31/32, TAF2G, TAFII-31, TAFII-32, TAFII31, TAFII32, TAFIID32 TATA-box binding protein associated factor 9 transcription initiation factor TFIID subunit 9|RNA polymerase II TBP-associated factor subunit G|TAF9 RNA polymerase II, TATA box binding protein (TBP)-associated factor, 32kDa|transcription initiation factor TFIID 31 kD subunit|transcription initiation factor TFIID 31 kDa subunit|transcription initiation factor TFIID 32 kDa subunit
*If product is indicated to react with multiple species, protein info is based on the gene entry specified above in "Species".For more info on TAF9, check out the TAF9 Infographic
We have 30,000+ of these available, one for each gene! Check them out.
In this infographic, you will see the following information for TAF9: database IDs, superfamily, protein function, synonyms, molecular weight, chromosomal locations, tissues of expression, subcellular locations, post-translational modifications, and related diseases, research areas & pathways. If you want to see more information included, or would like to contribute to it and be acknowledged, please contact [email protected].
Specific Publications For Anti-TAF9 Antibody (A05319)
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