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Product Info Summary
| SKU: | M00949 |
|---|---|
| Size: | 100 μl |
| Reactive Species: | Human, Mouse, Rat |
| Host: | Rabbit |
| Application: | Flow Cytometry, IF, IHC, ICC, WB |
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Product info
Product Name
Anti-Hsp70 HSPA1A Rabbit Monoclonal Antibody
View all HSP70/HSPA1A Antibodies
SKU/Catalog Number
M00949
Size
100 μl
Form
Liquid
Description
Boster Bio Anti-Hsp70 HSPA1A Rabbit Monoclonal Antibody catalog # M00949. Tested in WB, IHC, ICC/IF, Flow Cytometry applications. This antibody reacts with Human, Mouse, Rat.
Storage & Handling
Store at -20°C for one year. For short term storage and frequent use, store at 4°C for up to one month. Avoid repeated freeze-thaw cycles.
Cite This Product
Anti-Hsp70 HSPA1A Rabbit Monoclonal Antibody (Boster Biological Technology, Pleasanton CA, USA, Catalog # M00949)
Host
Rabbit
Contents
Rabbit IgG in phosphate buffered saline, pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol, 0.4-0.5mg/ml BSA.
Clonality
Monoclonal
Clone Number
DGE-8
Isotype
Rabbit IgG
Immunogen
A synthesized peptide derived from human Hsp70
*Blocking peptide can be purchased. Costs vary based on immunogen length. Contact us for pricing.
Reactive Species
M00949 is reactive to HSPA1A in Human, Mouse, Rat
Reconstitution
Restore with deionized water (or equivalent) for reconstitution volume of 1.0 mL
Observed Molecular Weight
70 kDa
Calculated molecular weight
70 kDa
Antibody Validation
Boster validates all antibodies on WB, IHC, ICC, Immunofluorescence, and ELISA with known positive control and negative samples to ensure specificity and high affinity, including thorough antibody incubations.
Application & Images
Applications
M00949 is guaranteed for Flow Cytometry, IF, IHC, ICC, WB Boster Guarantee
Assay Dilutions Recommendation
The recommendations below provide a starting point for assay optimization. The actual working concentration varies and should be decided by the user.
WB 1:500-1:2000
IHC 1:100-1:500
ICC/IF 1:50-1:100
FC 1:100
Positive Control
WB: human K562 whole cell, human 293T whole cell, human MCF-7 whole cell, human HepG2 whole cell, rat heart tissue, rat liver tissue, mouse heart tissue, mouse liver tissue
IHC: Human squamous carcinoma tissue, Human testis cancer tissue, Human prostate cancer tissue, human breast tissue
ICC/IF: Hela cell
Validation Images & Assay Conditions
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Figure 1. Western blot analysis of Hsp70 using anti-Hsp70 antibody (M00949).
Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel) / 90V (Resolving gel) for 2-3 hours. The sample well of each lane was loaded with 30 ug of sample under reducing conditions.
Lane 1: human K562 whole cell lysates,
Lane 2: human 293T whole cell lysates,
Lane 3: human MCF-7 whole cell lysates,
Lane 4: human HepG2 whole cell lysates,
Lane 5: rat heart tissue lysates,
Lane 6: rat liver tissue lysates,
Lane 7: mouse heart tissue lysates,
Lane 8: mouse liver tissue lysates.
After electrophoresis, proteins were transferred to a nitrocellulose membrane at 150 mA for 50-90 minutes. Blocked the membrane with 5% non-fat milk/TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-Hsp70 antigen affinity purified monoclonal antibody (M00949) at 1:500 overnight at 4°C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:500 for 1.5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit (Catalog # EK1002) with Tanon 5200 system. A specific band was detected for Hsp70 at approximately 70 kDa. The expected band size for Hsp70 is at 70 kDa.
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Immunohistochemical analysis of paraffin-embedded Human squamous carcinoma, using the Antibody at 1:200 dilution.
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Immunohistochemical analysis of paraffin-embedded Human testis cancer, using the Antibody at 1:200 dilution.
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Immunohistochemical analysis of paraffin-embedded Human prostate cancer, using the Antibody at 1:200 dilution.
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Immunohistochemical analysis of paraffin-embedded human breast, using Hsp70 Antibody .
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Immunofluorescent analysis of Hela cells, using Hsp70 Antibody .
Protein Target Info & Infographic
Gene/Protein Information For HSPA1A (Source: Uniprot.org, NCBI)
Gene Name
HSPA1A
Full Name
Heat shock 70 kDa protein 1A
Weight
70 kDa
Superfamily
heat shock protein 70 family
Alternative Names
Heat shock 70 kDa protein 1A ;Heat shock 70 kDa protein 1;HSP70-1 ;HSP70.1;HSPA1A;HSPA1, HSX70; HSPA1A HEL-S-103, HSP70-1, HSP70-1A, HSP70-2, HSP70.1, HSP70.2, HSP70I, HSP72, HSPA1 heat shock protein family A (Hsp70) member 1A heat shock 70 kDa protein 1A|HSP70-1/HSP70-2|HSP70.1/HSP70.2|Heat shock 70 kDa protein 1B|Heat shock 70 kDa protein 2|dnaK-type molecular chaperone HSP70-1|epididymis secretory protein Li 103|epididymis secretory sperm binding protein|heat shock 70 kDa protein 1|heat shock 70 kDa protein 1/2|heat shock 70 kDa protein 1A/1B|heat shock 70kD protein 1A|heat shock 70kDa protein 1A|heat shock-induced protein
*If product is indicated to react with multiple species, protein info is based on the gene entry specified above in "Species".For more info on HSPA1A, check out the HSPA1A Infographic
We have 30,000+ of these available, one for each gene! Check them out.
In this infographic, you will see the following information for HSPA1A: database IDs, superfamily, protein function, synonyms, molecular weight, chromosomal locations, tissues of expression, subcellular locations, post-translational modifications, and related diseases, research areas & pathways. If you want to see more information included, or would like to contribute to it and be acknowledged, please contact [email protected].
Specific Publications For Anti-Hsp70 HSPA1A Rabbit Monoclonal Antibody (M00949)
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Customer Q&As
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16 Customer Q&As for Anti-Hsp70 HSPA1A Rabbit Monoclonal Antibody
Question
My question regards to test anti-Hsp70 Rabbit Monoclonal antibody M00949 on mouse cervix carcinoma for research purposes, then I may be interested in using anti-Hsp70 Rabbit Monoclonal antibody M00949 for diagnostic purposes as well. Is the antibody suitable for diagnostic purposes?
Verified Customer
Verified customer
Asked: 2020-05-07
Answer
The products we sell, including anti-Hsp70 Rabbit Monoclonal antibody M00949, are only intended for research use. They would not be suitable for use in diagnostic work. If you have the means to develop a product into diagnostic use, and are interested in collaborating with us and develop our product into an IVD product, please contact us for more discussions.
Boster Scientific Support
Answered: 2020-05-07
Question
My question regarding product M00949, anti-Hsp70 Rabbit Monoclonal antibody. I was wondering if it would be possible to conjugate this antibody with biotin. I would need it to be without BSA or sodium azide. I am planning on using a buffer exchange of sodium azide with PBS only. Would there be problems for me to conjugate the antibody and store it in -20 degrees in small aliquots?
Verified Customer
Verified customer
Asked: 2020-03-04
Answer
We suggest not storing this antibody with PBS buffer only in -20 degrees. If you want to store it in -20 degrees it is best to add some cryoprotectant like glycerol. If you want carrier free M00949 anti-Hsp70 Rabbit Monoclonal antibody, we can provide it to you in a special formula with trehalose and/or glycerol. These molecules will not interfere with conjugation chemistry and provide a good level of protection for the antibody from degradation. Please be sure to specify this in your purchase order.
Boster Scientific Support
Answered: 2020-03-04
Question
We are currently using anti-Hsp70 Rabbit Monoclonal antibody M00949 for rat tissue, and we are satisfied with the WB results. The species of reactivity given in the datasheet says human, mouse, rat. Is it true that the antibody can work on canine tissues as well?
Verified Customer
Verified customer
Asked: 2020-01-22
Answer
The anti-Hsp70 Rabbit Monoclonal antibody (M00949) has not been tested for cross reactivity specifically with canine tissues, though there is a good chance of cross reactivity. We have an innovator award program that if you test this antibody and show it works in canine you can get your next antibody for free. Please contact me if I can help you with anything.
Boster Scientific Support
Answered: 2020-01-22
Question
Will anti-Hsp70 Rabbit Monoclonal antibody M00949 work for IF with cervix carcinoma?
Verified Customer
Verified customer
Asked: 2020-01-10
Answer
According to the expression profile of cervix carcinoma, HSPA1A is highly expressed in cervix carcinoma. So, it is likely that anti-Hsp70 Rabbit Monoclonal antibody M00949 will work for IF with cervix carcinoma.
Boster Scientific Support
Answered: 2020-01-10
Question
We need using your anti-Hsp70 Rabbit Monoclonal antibody for negative regulation of inclusion body assembly studies. Has this antibody been tested with western blotting on k562 cell lysate? We would like to see some validation images before ordering.
Verified Customer
Verified customer
Asked: 2019-12-05
Answer
Thank you for your inquiry. This M00949 anti-Hsp70 Rabbit Monoclonal antibody is validated on k562 cell lysate, 293t cell lysate. It is guaranteed to work for IF, IHC, WB in human, mouse, rat. Our Boster guarantee will cover your intended experiment even if the sample type has not been be directly tested.
Boster Scientific Support
Answered: 2019-12-05
Question
Our lab used your anti-Hsp70 Rabbit Monoclonal antibody for IF on embryonic kidney last year. I am using rat, and I plan to use the antibody for WB next. My lab would like examining embryonic kidney as well as muscle in our next experiment. Do you have any suggestion on which antibody would work the best for WB?
Verified Customer
Verified customer
Asked: 2019-11-28
Answer
I have checked the website and datasheets of our anti-Hsp70 Rabbit Monoclonal antibody and I see that M00949 has been tested on rat in both IF and WB. Thus M00949 should work for your application. Our Boster satisfaction guarantee will cover this product for WB in rat even if the specific tissue type has not been validated. We do have a comprehensive range of products for WB detection and you can check out our website bosterbio.com to find out more information about them.
Boster Scientific Support
Answered: 2019-11-28
Question
We were happy with the WB result of your anti-Hsp70 Rabbit Monoclonal antibody. However we have observed positive staining in endothelial cell cytoplasm. using this antibody. Is that expected? Could you tell me where is HSPA1A supposed to be expressed?
Verified Customer
Verified customer
Asked: 2019-09-23
Answer
Based on literature, endothelial cell does express HSPA1A. Generally HSPA1A expresses in cytoplasm. Regarding which tissues have HSPA1A expression, here are a few articles citing expression in various tissues:
Brain, Cajal-Retzius cell, and Fetal brain cortex, Pubmed ID: 23349634
Brain, Muscle, Pancreas, PNS, and Skin, Pubmed ID: 15489334
Cervix carcinoma, Pubmed ID: 17081983, 18691976, 20068231
Cervix carcinoma, and Erythroleukemia, Pubmed ID: 23186163
Colon carcinoma, Pubmed ID: 24129315
Liver, Pubmed ID: 24275569
Uterus, Pubmed ID: 14702039
Boster Scientific Support
Answered: 2019-09-23
Question
I was wanting to use your anti-Hsp70 Rabbit Monoclonal antibody for IF for mouse cervix carcinoma on frozen tissues, but I want to know if it has been tested for this particular application. Has this antibody been tested and is this antibody a good choice for mouse cervix carcinoma identification?
Verified Customer
Verified customer
Asked: 2019-05-13
Answer
As indicated on the product datasheet, M00949 anti-Hsp70 Rabbit Monoclonal antibody has been tested for IF, IHC, WB on human, mouse, rat tissues. We have an innovator award program that if you test this antibody and show it works in mouse cervix carcinoma in IHC-frozen, you can get your next antibody for free.
Boster Scientific Support
Answered: 2019-05-13
Question
Would M00949 anti-Hsp70 Rabbit Monoclonal antibody work on parafin embedded sections? If so, which fixation method do you recommend we use (PFA, paraformaldehyde, other)?
S. Bhatt
Verified customer
Asked: 2019-03-13
Answer
You can see on the product datasheet, M00949 anti-Hsp70 Rabbit Monoclonal antibody as been validated on IF. It is best to use PFA for fixation because it has better tissue penetration ability. PFA needs to be prepared fresh before use. Long term stored PFA turns into formalin, as the PFA molecules congregate and become formalin.
Boster Scientific Support
Answered: 2019-03-13
Question
We appreciate helping with my inquiry over the phone. Here are the WB image, lot number and protocol we used for cervix carcinoma using anti-Hsp70 Rabbit Monoclonal antibody M00949. Let me know if you need anything else.
Verified Customer
Verified customer
Asked: 2018-12-04
Answer
Thank you for the data. You have provided everything we needed. Our lab team are working to resolve your inquiry as quickly as possible, and we appreciate your patience and understanding! Please let me know if there is anything you need in the meantime.
Boster Scientific Support
Answered: 2018-12-04
Question
Here is the WB image, lot number and protocol we used for cervix carcinoma using anti-Hsp70 Rabbit Monoclonal antibody M00949. Please let me know if you require anything else.
Verified Customer
Verified customer
Asked: 2017-11-14
Answer
Thank you very much for the data. Our lab team are working to resolve this as quickly as possible, and we appreciate your patience and understanding! You have provided everything we needed. Please let me know if there is anything you need in the meantime.
Boster Scientific Support
Answered: 2017-11-14
Question
Is there a BSA free version of anti-Hsp70 Rabbit Monoclonal antibody M00949 available?
Verified Customer
Verified customer
Asked: 2017-07-25
Answer
We appreciate your recent telephone inquiry. I can confirm that some lots of this anti-Hsp70 Rabbit Monoclonal antibody M00949 are BSA free. For now, these lots are available and we can make a BSA free formula for you free of charge. It will take 3 extra days to prepare. If you require this antibody BSA free again in future, please do not hesitate to contact me and I will be pleased to check which lots we have in stock that are BSA free.
Boster Scientific Support
Answered: 2017-07-25
Question
Is a blocking peptide available for product anti-Hsp70 Rabbit Monoclonal antibody (M00949)?
T. Evans
Verified customer
Asked: 2015-03-09
Answer
We do provide the blocking peptide for product anti-Hsp70 Rabbit Monoclonal antibody (M00949). If you would like to place an order for it please contact [email protected] and make a special request.
Boster Scientific Support
Answered: 2015-03-09
Question
Is this M00949 anti-Hsp70 Rabbit Monoclonal antibody reactive to the isotypes of HSPA1A?
R. Jackson
Verified customer
Asked: 2015-02-27
Answer
The immunogen of M00949 anti-Hsp70 Rabbit Monoclonal antibody is A synthesized peptide derived from human Hsp70 . Could you tell me which isotype you are interested in so I can help see if the immunogen is part of this isotype?
Boster Scientific Support
Answered: 2015-02-27
Question
I see that the anti-Hsp70 Rabbit Monoclonal antibody M00949 works with IF, what is the protocol used to produce the result images on the product page?
A. Moore
Verified customer
Asked: 2014-10-30
Answer
You can find protocols for IF on the "support/technical resources" section of our navigation menu. If you have any further questions, please send an email to [email protected]
Boster Scientific Support
Answered: 2014-10-30
Question
We have been able to see staining in mouse liver. Are there any suggestions? Is anti-Hsp70 Rabbit Monoclonal antibody supposed to stain liver positively?
M. Anderson
Verified customer
Asked: 2014-07-24
Answer
According to literature liver does express HSPA1A. According to Uniprot.org, HSPA1A is expressed in endothelial cell, uterus, brain, muscle, pancreas, pns skin, embryonic kidney, brain, cajal-retzius cell fetal brain cortex, cervix carcinoma, cervix carcinoma erythroleukemia, liver, colon carcinoma, among other tissues. Regarding which tissues have HSPA1A expression, here are a few articles citing expression in various tissues:
Brain, Cajal-Retzius cell, and Fetal brain cortex, Pubmed ID: 23349634
Brain, Muscle, Pancreas, PNS, and Skin, Pubmed ID: 15489334
Cervix carcinoma, Pubmed ID: 17081983, 18691976, 20068231
Cervix carcinoma, and Erythroleukemia, Pubmed ID: 23186163
Colon carcinoma, Pubmed ID: 24129315
Liver, Pubmed ID: 24275569
Uterus, Pubmed ID: 14702039
Boster Scientific Support
Answered: 2014-07-24
