Anti-TAF1/Kat4 Antibody

Product Name

Anti-TAF1/Kat4 Antibody

View all KAT4/TBP Associated Factor 1 Antibodies

SKU/Catalog Number

A02151

Size

100ug

Form

Liquid (sterile filtered)

Description

Boster Bio Anti-TAF1/Kat4 Antibody (Catalog # A02151). Tested in ChIP, ELISA, WB applications. This antibody reacts with Human, Mouse.

Storage & Handling

Store vial at -20°C prior to opening. Aliquot contents and freeze at -20°C or below for extended storage. Avoid cycles of freezing and thawing. Centrifuge product if not completely clear after standing at room temperature. This product is stable for several weeks at 4°C as an undiluted liquid. Dilute only prior to immediate use. Expiration date is one (1) year from date of opening. (Ship on dry ice.)

Cite This Product

Anti-TAF1/Kat4 Antibody (Boster Biological Technology, Pleasanton CA, USA, Catalog # A02151)

Host

Rabbit

Contents

0.02 M Potassium Phosphate, 0.15 M Sodium Chloride, pH 7.2, 0.01% (w/v) Sodium Azide

Clonality

Polyclonal

Isotype

IgG

Immunogen

This affinity purified antibody was prepared from whole rabbit serum produced by repeated immunizations with a synthetic peptide corresponding to amino acids near the carboxyl terminus of human TAF1.

*Blocking peptide can be purchased. Costs vary based on immunogen length. Contact us for pricing.

Reactive Species

A02151 is reactive to TAF1 in Human, Mouse

Applications

A02151 is guaranteed for ChIP, ELISA, WB Boster Guarantee

Observed Molecular Weight

42 kDa

Calculated molecular weight

212677 MW

Background of KAT4/TBP Associated Factor 1

Anti-TAF1 antibody is suitable for Cancer, Immunology and Nuclear Signaling research. Transcription Initiation Factor TFIID Subunit 1 (TAF1) is the largest component and core scaffold of the TFIID basal transcription factor complex, which also includes TATA-binding protein (TBP) and a variety of TBP-associated factors. TFIID nucleates the formation of transcription pre-initiation complexes and plays a key role in the regulation of gene expression by RNA polymerase II. TAF1 possesses DNA-binding activity and also contains novel N- and C-terminal Ser/Thr kinase domains which can auto-phosphorylate or trans-phosphorylate other transcription factors. For example, TAF1 interacts with the C-terminus of TP53 and phosphorylates the T55 residue, leading to MDM2-mediated degradation of TP53.  TAF1 also catalyzes Ser phosphorylation of general transcription factor IIA (GTF2A1) and IIF (GTF2F1). The retinoblastoma tumor suppressor protein, RB1, interacts with the N-terminal domain of TAF1 and inhibits its intrinsic kinase activity. TAF1 is essential for progression through the G1 phase of the cell cycle and has been reported to be indispensable for expression of 18% of mammalian genes.

Antibody Validation

Boster validates all antibodies on WB, IHC, ICC, Immunofluorescence, and ELISA with known positive control and negative samples to ensure specificity and high affinity, including thorough antibody incubations.

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Assay Dilutions Recommendation

The recommendations below provide a starting point for assay optimization. The actual working concentration varies and should be decided by the user.

ELISA: 1:150,000 - 1:300,000
ChIP: User optimized
IHC: 10µg/ml
IP: User optimized
WB: 1:100 - 1:2,000

Validation Images & Assay Conditions

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TAF1 was detected in paraffin-embedded sections of human prostate tissues using rabbit anti-TAF1 Antigen Affinity purified polyclonal antibody (Catalog # A02151) at 10 μg/mL. Secondary antibody: Peroxidase rabbit secondary antibody at 1:10,000 for 45 min at RT. Staining: TAF-1 as precipitated red signal with hematoxylin purple nuclear counterstain.

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Western blot analysis of TAF1 expression in Marker: Opal Pre-stained ladder. Lane 1: HEK293 lysate. Lane 2: HELA Lysate. Lane 3: MCF-7 Lysate. Lane 4: JURKAT Lysate. Lane 5: A431 Lysate. Lane 6: A549 Lysate. Lane 7: LNCap Lysate. Lane 8: MOLT-4 Lysate. Lane 9: Ramos Lysate. Lane 10: RAJI Lsyate. Lane 11: A-172 Lysate. Lane 12: NIH3T3 Lysate. TAF1 at 250KD was detected using rabbit anti-TAF1 Antigen Affinity purified polyclonal antibody (Catalog # A02151) at 0.2 μg/mL. The blot was developed using chemiluminescence (ECL) method (Catalog # EK1002).

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Western blot analysis of TAF1 expression in HeLa nuclear extract (arrowhead). The membrane was probed with the primary antibody at dilutions of 1:100 (lane 1), 1:250 (lane 2), 1: 500 (Lane 3 and 1:1,000 (Lane 4). TAF1 was detected using rabbit anti-TAF1 Antigen Affinity purified polyclonal antibody (Catalog # A02151). Personal Communication, Anne Gegonne, CCR-NCI, Bethesda, MD.

Gene/Protein Information For TAF1 (Source: Uniprot.org, NCBI)

Alternative Names

250kDa; BA2Rdystonia 3 (with Parkinsonism); CCG1TATA box binding protein (TBP)-associated factor, RNA polymerase II, A, 250kD; CCGS; Cell cycle gene 1 protein; cell cycle, G1 phase defect; complementation of cell cycle block, G1-to-S; DYT3/TAF1; EC 2.7.11; EC 2.7.11.1; KAT4; NSCL2; OF; P250; TAF(II)250; TAF1 RNA polymerase II, TATA box binding protein (TBP)-associated factor; TAF2AN-TAF1; TAFII-250; TAFII250DYT3; TBP-associated factor 250 kDa; transcription factor TFIID p250 polypeptide; Transcription initiation factor TFIID 250 kDa subunit; transcription initiation factor TFIID subunit 1; XDP TAF1 BA2R, CCG1, CCGS, DYT3, DYT3/TAF1, KAT4, MRXS33, N-TAF1, NSCL2, OF, P250, TAF(II)250, TAF2A, TAFII-250, TAFII250, XDP TATA-box binding protein associated factor 1 transcription initiation factor TFIID subunit 1|TAF1 RNA polymerase II, TATA box binding protein (TBP)-associated factor, 250kDa|TBP-associated factor 250 kDa|cell cycle gene 1 protein|cell cycle, G1 phase defect|complementation of cell cycle block, G1-to-S|transcription factor TFIID p250 polypeptide

For more info on TAF1, check out the TAF1 Infographic

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In this infographic, you will see the following information for TAF1: database IDs, superfamily, protein function, synonyms, molecular weight, chromosomal locations, tissues of expression, subcellular locations, post-translational modifications, and related diseases, research areas & pathways. If you want to see more information included, or would like to contribute to it and be acknowledged, please contact [email protected].

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