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Product Info Summary
| SKU: | P02314 |
|---|---|
| Size: | 100 µl |
| Reactive Species: | Rat |
| Host: | Rabbit |
| Application: | ICC, WB |
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Product info
Product Name
Anti-Phospho-Synaptotagmin (Thr202) Syt1 Antibody
View all Synaptotagmin 1 Antibodies
SKU/Catalog Number
P02314
Size
100 µl
Form
Liquid
Description
Boster Bio Anti-Phospho-Synaptotagmin (Thr202) Syt1 Antibody (Catalog # P02314). Tested in WB, ICC applications. This antibody reacts with Rat.
Storage & Handling
Storage at -20°C is recommended, as aliquots may be taken without freeze/thawing due to presence of 50% glycerol. Stable for at least 1 year at -20°C. After date of receipt, stable for at least 1 year at -20°C.
Cite This Product
Anti-Phospho-Synaptotagmin (Thr202) Syt1 Antibody (Boster Biological Technology, Pleasanton CA, USA, Catalog # P02314)
Host
Rabbit
Contents
10 mM HEPES (pH 7.5), 150 mM NaCl, 100 µg per ml BSA and 50% glycerol.
Clonality
Polyclonal
Isotype
IgG
Immunogen
Synthetic phospho-peptide corresponding to amino acid residues surrounding Thr202 of rat synaptotagmin, conjugated to keyhole limpet hemocyanin (KLH). Immunogen species is Rat.
*Blocking peptide can be purchased. Costs vary based on immunogen length. Contact us for pricing.
Cross-reactivity
No cross reactivity with other proteins.
Reactive Species
P02314 is reactive to SYT1 in Rat
Reconstitution
Observed Molecular Weight
52 kDa
Calculated molecular weight
47399 MW
Background of Synaptotagmin 1
Synaptotagmin is widely regarded as the primary calcium sensor for synaptic vesicle exocytosis (Fernandez-Chacon et al., 2001; Wang et al., 2003). Moreover, recent studies indicate that the protein also plays a key role in endocytosis (Poskanzer et al., 2003). Synaptotagmin can be phosphorylated by multiple protein kinases and this may play a key role in modulation of synaptotagmin’s ability to influence both the exocytotic and endocytotic components of synaptic transmission (Hilfiker et al., 1999; Lee et al., 2004).
Antibody Validation
Boster validates all antibodies on WB, IHC, ICC, Immunofluorescence, and ELISA with known positive control and negative samples to ensure specificity and high affinity, including thorough antibody incubations.
Application & Images
Applications
P02314 is guaranteed for ICC, WB Boster Guarantee
Assay Dilutions Recommendation
The recommendations below provide a starting point for assay optimization. The actual working concentration varies and should be decided by the user.
WB: 1:1000
ICC: 1:400
Validation Images & Assay Conditions
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Immunostaining of 14 DIV rat cortical neurons showing synaptotagmin when phosphorylated at Thr202 in red and PSD95 in green.
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Western blot of rat cortical lysate showing specific immunolabeling of the ~62 kDa synaptotagmin phosphorylated at Thr202 in the first lane (-). Phosphospecificity is shown in the second lane (+) where the immunolabeling is completely eliminated by blot treatment with lambda phosphatase (gamma-Ptase, 1200 units for 30 minutes).
Protein Target Info & Infographic
Gene/Protein Information For SYT1 (Source: Uniprot.org, NCBI)
Gene Name
SYT1
Full Name
Synaptotagmin-1
Weight
47399 MW
Superfamily
synaptotagmin family
Alternative Names
DKFZp781D2042 antibody, FLJ42519 antibody, P65 antibody, SVP65 antibody, synaptotagmin 1 antibody, Synaptotagmin I antibody, SYT antibody, SYT1 antibody, SytI antibody SYT1 BAGOS, P65, SVP65, SYT synaptotagmin 1 synaptotagmin-1|synaptotagmin I|sytI
*If product is indicated to react with multiple species, protein info is based on the gene entry specified above in "Species".For more info on SYT1, check out the SYT1 Infographic
We have 30,000+ of these available, one for each gene! Check them out.
In this infographic, you will see the following information for SYT1: database IDs, superfamily, protein function, synonyms, molecular weight, chromosomal locations, tissues of expression, subcellular locations, post-translational modifications, and related diseases, research areas & pathways. If you want to see more information included, or would like to contribute to it and be acknowledged, please contact [email protected].
Specific Publications For Anti-Phospho-Synaptotagmin (Thr202) Syt1 Antibody (P02314)
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No publications found for P02314
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Customer Q&As
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4 Customer Q&As for Anti-Phospho-Synaptotagmin (Thr202) Syt1 Antibody
Question
We are currently using anti-Phospho-Synaptotagmin (Thr202) antibody P02314 for rat tissue, and we are satisfied with the IHC results. The species of reactivity given in the datasheet says rat. Is it possible that the antibody can work on primate tissues as well?
Verified Customer
Verified customer
Asked: 2019-08-12
Answer
The anti-Phospho-Synaptotagmin (Thr202) antibody (P02314) has not been tested for cross reactivity specifically with primate tissues, but there is a good chance of cross reactivity. We have an innovator award program that if you test this antibody and show it works in primate you can get your next antibody for free. Please contact me if I can help you with anything.
Boster Scientific Support
Answered: 2019-08-12
Question
We have seen staining in rat amygdala brain. Any tips? Is anti-Phospho-Synaptotagmin (Thr202) antibody supposed to stain amygdala brain positively?
Verified Customer
Verified customer
Asked: 2019-07-16
Answer
From literature amygdala brain does express SYT1. From Uniprot.org, SYT1 is expressed in middle temporal gyrus, amygdala brain, hippocampus, pns, among other tissues. Regarding which tissues have SYT1 expression, here are a few articles citing expression in various tissues:
Amygdala, and Brain, Pubmed ID: 14702039
Hippocampus, Pubmed ID: 17974005
PNS, Pubmed ID: 15489334
Boster Scientific Support
Answered: 2019-07-16
Question
We ordered your anti-Phospho-Synaptotagmin (Thr202) antibody for WB on hippocampus last year. I am using rat, and We are going to use the antibody for IHC next. My question regards examining hippocampus as well as amygdala brain in our next experiment. Do you have any suggestion on which antibody would work the best for IHC?
E. Patel
Verified customer
Asked: 2016-09-20
Answer
I have checked the website and datasheets of our anti-Phospho-Synaptotagmin (Thr202) antibody and it seems that P02314 has been tested on rat in both WB and IHC. Thus P02314 should work for your application. Our Boster satisfaction guarantee will cover this product for IHC in rat even if the specific tissue type has not been validated. We do have a comprehensive range of products for IHC detection and you can check out our website bosterbio.com to find out more information about them.
Boster Scientific Support
Answered: 2016-09-20
Question
Our lab were well pleased with the WB result of your anti-Phospho-Synaptotagmin (Thr202) antibody. However we have been able to see positive staining in amygdala brain cytoplasmic vesicle using this antibody. Is that expected? Could you tell me where is SYT1 supposed to be expressed?
J. Miller
Verified customer
Asked: 2013-12-30
Answer
According to literature, amygdala brain does express SYT1. Generally SYT1 expresses in cytoplasmic vesicle, secretory vesicle. Regarding which tissues have SYT1 expression, here are a few articles citing expression in various tissues:
Amygdala, and Brain, Pubmed ID: 14702039
Hippocampus, Pubmed ID: 17974005
PNS, Pubmed ID: 15489334
Boster Scientific Support
Answered: 2013-12-30
