Anti-Synaptotagmin Syt1 Antibody (Monoclonal, ASV30)

Product Name

Anti-Synaptotagmin Syt1 Antibody (Monoclonal, ASV30)

View all Synaptotagmin 1 Antibodies

SKU/Catalog Number

M02314

Size

50μg/vial

Form

Liquid

Description

Boster Bio Anti-Synaptotagmin Syt1 Antibody (Monoclonal, ASV30) catalog # M02314. Tested in WB applications. This antibody reacts with Human, Mouse, Rat.

Storage & Handling

Store at -20°C for one year. For short term storage and frequent use, store at 4°C for up to one month. Avoid repeated freeze-thaw cycles.

Cite This Product

Anti-Synaptotagmin Syt1 Antibody (Monoclonal, ASV30) (Boster Biological Technology, Pleasanton CA, USA, Catalog # M02314)

Host

Mouse

Contents

Each vial contains 50% glycerol and 0.09% sodium azide.

Clonality

Monoclonal

Clone Number

ASV30

Isotype

Mouse IgG

Immunogen

Rat brain synaptic junction protein complexes .

*Blocking peptide can be purchased. Costs vary based on immunogen length. Contact us for pricing.

Cross-reactivity

Detects ~20kDa. Does not cross-react with αB-crystallin, βL-crystallin, ΒH- crystallin, γ-crystallin, HSP25, HSP27 or HSP47 proteins.

Reactive Species

M02314 is reactive to Syt1 in Human, Mouse, Rat

Reconstitution

Observed Molecular Weight

68 kDa

Calculated molecular weight

47399 MW

Background of Synaptotagmin 1

The alpha-crystallins are major water-soluble lens structural proteins of the vertebrate eye that are related to the small heat shock protein family. The alpha-crystallins possess structural and functional similarities with HSP25 and HSP27 (1). Mammalian lens cystallins are divided into alpha, beta and gamma families. Alpha and beta families are further divided into acidic and basic groups (Alpha-A and Alpha-B respectively). In the lens, alpha-crystallin primarily functions to maintain proper refractive index, however it can also function as a molecular chaperone that binds to the denatured proteins, keeping them in solution and thereby maintaining the translucency of the lens. When cellular stress occurs, alpha-crystallin enters its' phosphorylated state and may serve a structural control function and play a role in protein maintenance (2). In addition to their interaction with proteins, alpha-crystallins also interact with native molecules such as membrane proteins, Golgi matrix protein, structural proteins, nuclear proteins and DNA (3, 4, 5, 6, and 7). Two other functions are an autokinase activity and participation in the intracellular architecture, and it has also been proven that both alpha-A and B prevent apoptosis by inhibiting caspases (8).

Antibody Validation

Boster validates all antibodies on WB, IHC, ICC, Immunofluorescence, and ELISA with known positive control and negative samples to ensure specificity and high affinity, including thorough antibody incubations.

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Applications

M02314 is guaranteed for WB Boster Guarantee

Assay Dilutions Recommendation

The recommendations below provide a starting point for assay optimization. The actual working concentration varies and should be decided by the user.

WB, 1:1, 000, colorimetric, Human, Mouse, Rat

Validation Images & Assay Conditions

Gene/Protein Information For Syt1 (Source: Uniprot.org, NCBI)

Alternative Names

Synaptotagmin-1;Synaptotagmin I;SytI;p65;Syt1; SYT1 BAGOS, P65, SVP65, SYT synaptotagmin 1 synaptotagmin-1|synaptotagmin I|sytI

*If product is indicated to react with multiple species, protein info is based on the gene entry specified above in "Species".

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